Loss of infectivity of HIV-1 particles produced by mobile lymphocytes.

HIV-1 spreads by cell-free particles and through direct cell contacts. To discriminate between these two modes of dissemination, an assay in which the cells are cultured under shaking conditions impairing cell-to-cell transmission has been described. We addressed the impact of shaking on HIV-1 parti...

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Autores principales:	Maxime Chazal, Patrycja Nzounza, Claudine Pique, Bertha Cecilia Ramirez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9c80d93be13e4199a8fb091c916c86a4
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spelling	oai:doaj.org-article:9c80d93be13e4199a8fb091c916c86a42021-11-25T05:57:31ZLoss of infectivity of HIV-1 particles produced by mobile lymphocytes.1932-620310.1371/journal.pone.0109601https://doaj.org/article/9c80d93be13e4199a8fb091c916c86a42014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0109601https://doaj.org/toc/1932-6203HIV-1 spreads by cell-free particles and through direct cell contacts. To discriminate between these two modes of dissemination, an assay in which the cells are cultured under shaking conditions impairing cell-to-cell transmission has been described. We addressed the impact of shaking on HIV-1 particle infectivity. Kinetics of HIV-1 infection in static or shaking conditions confirmed that HIV-1 replication is reduced in mobile lymphocyte T cells. Strikingly, the infectivity of viruses produced by mobile lymphocytes was dramatically reduced. In parallel, the amount of envelope protein present on these particles showed a continuous decrease over time. We conclude that inefficient HIV-1 replication in mobile lymphocytes in this experimental system is not only due to avoidance of viral cell-to-cell transfer but also to the loss of infectivity of the viral particles due to the alteration of the composition and functionality of the particles produced by these lymphocytes. It is important to take these observations into account when studying viral transmission under shaking conditions.Maxime ChazalPatrycja NzounzaClaudine PiqueBertha Cecilia RamirezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e109601 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maxime Chazal Patrycja Nzounza Claudine Pique Bertha Cecilia Ramirez Loss of infectivity of HIV-1 particles produced by mobile lymphocytes.
description	HIV-1 spreads by cell-free particles and through direct cell contacts. To discriminate between these two modes of dissemination, an assay in which the cells are cultured under shaking conditions impairing cell-to-cell transmission has been described. We addressed the impact of shaking on HIV-1 particle infectivity. Kinetics of HIV-1 infection in static or shaking conditions confirmed that HIV-1 replication is reduced in mobile lymphocyte T cells. Strikingly, the infectivity of viruses produced by mobile lymphocytes was dramatically reduced. In parallel, the amount of envelope protein present on these particles showed a continuous decrease over time. We conclude that inefficient HIV-1 replication in mobile lymphocytes in this experimental system is not only due to avoidance of viral cell-to-cell transfer but also to the loss of infectivity of the viral particles due to the alteration of the composition and functionality of the particles produced by these lymphocytes. It is important to take these observations into account when studying viral transmission under shaking conditions.
format	article
author	Maxime Chazal Patrycja Nzounza Claudine Pique Bertha Cecilia Ramirez
author_facet	Maxime Chazal Patrycja Nzounza Claudine Pique Bertha Cecilia Ramirez
author_sort	Maxime Chazal
title	Loss of infectivity of HIV-1 particles produced by mobile lymphocytes.
title_short	Loss of infectivity of HIV-1 particles produced by mobile lymphocytes.
title_full	Loss of infectivity of HIV-1 particles produced by mobile lymphocytes.
title_fullStr	Loss of infectivity of HIV-1 particles produced by mobile lymphocytes.
title_full_unstemmed	Loss of infectivity of HIV-1 particles produced by mobile lymphocytes.
title_sort	loss of infectivity of hiv-1 particles produced by mobile lymphocytes.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/9c80d93be13e4199a8fb091c916c86a4
work_keys_str_mv	AT maximechazal lossofinfectivityofhiv1particlesproducedbymobilelymphocytes AT patrycjanzounza lossofinfectivityofhiv1particlesproducedbymobilelymphocytes AT claudinepique lossofinfectivityofhiv1particlesproducedbymobilelymphocytes AT berthaceciliaramirez lossofinfectivityofhiv1particlesproducedbymobilelymphocytes
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Loss of infectivity of HIV-1 particles produced by mobile lymphocytes.

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