COMPARISON EFFECT BETWEEN PEGAGAN (Centella asiatica) EXTRACT AND PRAMIPEXOLE TOWARD LOCOMOTOR ACTIVITIES, α - SYNUCLEIN, AND NRF2 EXPRESSION IN ZEBRAFISH PARKINSON MODEL

Background: Parkinson disease is characterized with deposition of Lewy Bodies containing α–synuclein happened due to the effect of chronic neuroinflammation that causes the death of dopaminergic neurons through oxidative stress processes, so it involves the response of Nuclear factor erythroid 2-lik...

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Autores principales:	Ayu Trisnawati, Anasrulloh Anasrulloh, Sri Budhi Rianawati, Husnul Khotimah, Mulyohadi Ali, Budi Susetya
Formato:	article
Lenguaje:	EN ID
Publicado:	University of Brawijaya 2019
Materias:	centella asiatica locomotor activity α–synuclein nrf2 parkinson disease zebrafish Medicine R Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/9c84885d509f4c5b80334d89f5e4012f
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spelling	oai:doaj.org-article:9c84885d509f4c5b80334d89f5e4012f2021-11-12T05:56:38Z COMPARISON EFFECT BETWEEN PEGAGAN (Centella asiatica) EXTRACT AND PRAMIPEXOLE TOWARD LOCOMOTOR ACTIVITIES, α - SYNUCLEIN, AND NRF2 EXPRESSION IN ZEBRAFISH PARKINSON MODEL10.21776/ub.mnj.2019.005.01.22407-67242442-5001https://doaj.org/article/9c84885d509f4c5b80334d89f5e4012f2019-01-01T00:00:00Zhttps://mnj.ub.ac.id/index.php/mnj/article/view/291https://doaj.org/toc/2407-6724https://doaj.org/toc/2442-5001Background: Parkinson disease is characterized with deposition of Lewy Bodies containing α–synuclein happened due to the effect of chronic neuroinflammation that causes the death of dopaminergic neurons through oxidative stress processes, so it involves the response of Nuclear factor erythroid 2-like 2 (Nrf2). Centella asiatica (C.asiatica) contains antioxidant effect, inhibits the aggregation of α–synuclein and improves the locomotor on Parkinson-model animals so it needs to compare to the standard medication. Objective: To compare the C. asiatica extract and Pramipexole to the zebrafish Parkinson model by determining the locomotor activity, α–synuclein expression, and Nrf2. Methods: This study used six groups of zebrafish: negative control, rotenone rotenone [5 μg/L], pramipexole1, 2, 3 (rotenone + pramipexole [3,5] ng/mL, [7] ng/mL, [14] ng/mL), and C. asiatica (rotenone + C. asiatica [10] μg/mL). The observations of locomotor activity of day 0, 14, and 28 were continued to the α–synuclein immunohistochemical examination, and Nrf2 on the midbrain area. Results: There are significant differences in locomotor activity on day 28 among the C. asiatica group with rotenone (p<0,05), while there are no significant differences among the C. asiatica group with pramipexole [7] ng/mL and [14] ng/mL (p>0,05). α–synuclein expression of the C. asiatica group is the lowest and significantly different from all groups (p<0,05), while Nr2 had no significant differences (p>0,05). Conclusion: C. asiatica extract [10] μg/mL is equal to pramipexole [7] ng/mL and [14] ng/mL in improving locomotor activity, but C. asiatica extract holds excellence as it decreases α–synuclein expression better than pramipexole, while Nrf2 expression shows no differences.Ayu TrisnawatiAnasrulloh Anasrulloh Sri Budhi RianawatiHusnul KhotimahMulyohadi AliBudi SusetyaUniversity of Brawijayaarticlecentella asiaticalocomotor activityα–synucleinnrf2parkinson diseasezebrafishMedicineRNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENIDMalang Neurology Journal, Vol 5, Iss 1, Pp 5-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN ID
topic	centella asiatica locomotor activity α–synuclein nrf2 parkinson disease zebrafish Medicine R Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	centella asiatica locomotor activity α–synuclein nrf2 parkinson disease zebrafish Medicine R Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Ayu Trisnawati Anasrulloh Anasrulloh Sri Budhi Rianawati Husnul Khotimah Mulyohadi Ali Budi Susetya COMPARISON EFFECT BETWEEN PEGAGAN (Centella asiatica) EXTRACT AND PRAMIPEXOLE TOWARD LOCOMOTOR ACTIVITIES, α - SYNUCLEIN, AND NRF2 EXPRESSION IN ZEBRAFISH PARKINSON MODEL
description	Background: Parkinson disease is characterized with deposition of Lewy Bodies containing α–synuclein happened due to the effect of chronic neuroinflammation that causes the death of dopaminergic neurons through oxidative stress processes, so it involves the response of Nuclear factor erythroid 2-like 2 (Nrf2). Centella asiatica (C.asiatica) contains antioxidant effect, inhibits the aggregation of α–synuclein and improves the locomotor on Parkinson-model animals so it needs to compare to the standard medication. Objective: To compare the C. asiatica extract and Pramipexole to the zebrafish Parkinson model by determining the locomotor activity, α–synuclein expression, and Nrf2. Methods: This study used six groups of zebrafish: negative control, rotenone rotenone [5 μg/L], pramipexole1, 2, 3 (rotenone + pramipexole [3,5] ng/mL, [7] ng/mL, [14] ng/mL), and C. asiatica (rotenone + C. asiatica [10] μg/mL). The observations of locomotor activity of day 0, 14, and 28 were continued to the α–synuclein immunohistochemical examination, and Nrf2 on the midbrain area. Results: There are significant differences in locomotor activity on day 28 among the C. asiatica group with rotenone (p<0,05), while there are no significant differences among the C. asiatica group with pramipexole [7] ng/mL and [14] ng/mL (p>0,05). α–synuclein expression of the C. asiatica group is the lowest and significantly different from all groups (p<0,05), while Nr2 had no significant differences (p>0,05). Conclusion: C. asiatica extract [10] μg/mL is equal to pramipexole [7] ng/mL and [14] ng/mL in improving locomotor activity, but C. asiatica extract holds excellence as it decreases α–synuclein expression better than pramipexole, while Nrf2 expression shows no differences.
format	article
author	Ayu Trisnawati Anasrulloh Anasrulloh Sri Budhi Rianawati Husnul Khotimah Mulyohadi Ali Budi Susetya
author_facet	Ayu Trisnawati Anasrulloh Anasrulloh Sri Budhi Rianawati Husnul Khotimah Mulyohadi Ali Budi Susetya
author_sort	Ayu Trisnawati
title	COMPARISON EFFECT BETWEEN PEGAGAN (Centella asiatica) EXTRACT AND PRAMIPEXOLE TOWARD LOCOMOTOR ACTIVITIES, α - SYNUCLEIN, AND NRF2 EXPRESSION IN ZEBRAFISH PARKINSON MODEL
title_short	COMPARISON EFFECT BETWEEN PEGAGAN (Centella asiatica) EXTRACT AND PRAMIPEXOLE TOWARD LOCOMOTOR ACTIVITIES, α - SYNUCLEIN, AND NRF2 EXPRESSION IN ZEBRAFISH PARKINSON MODEL
title_full	COMPARISON EFFECT BETWEEN PEGAGAN (Centella asiatica) EXTRACT AND PRAMIPEXOLE TOWARD LOCOMOTOR ACTIVITIES, α - SYNUCLEIN, AND NRF2 EXPRESSION IN ZEBRAFISH PARKINSON MODEL
title_fullStr	COMPARISON EFFECT BETWEEN PEGAGAN (Centella asiatica) EXTRACT AND PRAMIPEXOLE TOWARD LOCOMOTOR ACTIVITIES, α - SYNUCLEIN, AND NRF2 EXPRESSION IN ZEBRAFISH PARKINSON MODEL
title_full_unstemmed	COMPARISON EFFECT BETWEEN PEGAGAN (Centella asiatica) EXTRACT AND PRAMIPEXOLE TOWARD LOCOMOTOR ACTIVITIES, α - SYNUCLEIN, AND NRF2 EXPRESSION IN ZEBRAFISH PARKINSON MODEL
title_sort	comparison effect between pegagan (centella asiatica) extract and pramipexole toward locomotor activities, α - synuclein, and nrf2 expression in zebrafish parkinson model
publisher	University of Brawijaya
publishDate	2019
url	https://doaj.org/article/9c84885d509f4c5b80334d89f5e4012f
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COMPARISON EFFECT BETWEEN PEGAGAN (Centella asiatica) EXTRACT AND PRAMIPEXOLE TOWARD LOCOMOTOR ACTIVITIES, α - SYNUCLEIN, AND NRF2 EXPRESSION IN ZEBRAFISH PARKINSON MODEL

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