N6-Methyladenosine-Related Long Noncoding RNAs as Potential Prognosis Biomarkers for Endometrial Cancer

Rui Shi, Ziwei Wang, Jun Zhang, Zhicheng Yu, Lanfen An, Sitian Wei, Dilu Feng, Hongbo Wang Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430022, People’s Republic of ChinaCorrespondence: Dilu Feng; Hongbo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shi R, Wang Z, Zhang J, Yu Z, An L, Wei S, Feng D, Wang H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	n6-methylandenosine long noncoding rnas endometrial cancer tumour microenvironment Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9c8aa7dfe9c748359b029735fde8e2e4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9c8aa7dfe9c748359b029735fde8e2e4
record_format	dspace
spelling	oai:doaj.org-article:9c8aa7dfe9c748359b029735fde8e2e42021-11-16T18:47:50ZN6-Methyladenosine-Related Long Noncoding RNAs as Potential Prognosis Biomarkers for Endometrial Cancer1178-7074https://doaj.org/article/9c8aa7dfe9c748359b029735fde8e2e42021-11-01T00:00:00Zhttps://www.dovepress.com/n6-methyladenosine-related-long-noncoding-rnas-as-potential-prognosis--peer-reviewed-fulltext-article-IJGMhttps://doaj.org/toc/1178-7074Rui Shi, Ziwei Wang, Jun Zhang, Zhicheng Yu, Lanfen An, Sitian Wei, Dilu Feng, Hongbo Wang Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430022, People’s Republic of ChinaCorrespondence: Dilu Feng; Hongbo WangDepartment of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430022, People’s Republic of ChinaTel +86 027 85350583Fax +86 278 572 6362Email fengdilu@126.com; hb_wang1969@sina.comPurpose: Endometrial cancer (EC) is a common gynaecologic malignancy with an increasing incidence rate and mortality in recent years. N6-methylandenosine (m6A)-related long noncoding RNA (lncRNA) plays a vital role in EC, emerging as one of the most abundant RNA modifications.Materials and Methods: The Cancer Genome Atlas (TCGA) database and UCSC Xena were used to download data related to EC. Survival and univariate and multifactorial prognostic analyses were performed for m6A-related lncRNAs. The expression levels of the three lncRNAs were verified using q-PCR. A nomogram was used to create a clinical tool to assess overall survival. To investigate the relationship between m6A-related lncRNA and EC, we downloaded differential genes related to EC from the TCGA database and mined three m6A-related lncRNAs, namely SCARNA9, TRAF3IP2-AS1, and AL133243.2. The data were categorized into high- and low-risk groups based on m6A-associated lncRNA.Results: Survival analysis revealed that the high-risk group had a lower survival rate. Survival analysis of three m6A-associated lncRNAs revealed that cases with high expression of SCARNA9 tended to have a poorer prognosis, whereas the opposite was true for TRAF3IP2-AS1, AL133243.2. Univariate and multifactorial prognostic analyses suggested statistical differences in patients’ age, FIGO stage, pathological grade, risk score, and prognosis of EC, which was confirmed by results of the separate prognostic factor analysis for the three lncRNAs. Risk status was validated as an independent prognostic indicator, and the prognostic nomogram combined patient age, pathological stage, and FIGO classification to assess 3– 5-year survival. Cases from high- and low-risk groups were analysed for the tumour microenvironment and immune cell scores, and stromal cell scores were found to be lower in the high-risk group. Correlations were analysed using different databases for immune cell classification.Conclusion: m6A-related lncRNAs may play a key role in the diagnosis and treatment of EC as targets of prognosis and the immune microenvironment.Keywords: N6-methylandenosine, long noncoding RNAs, endometrial cancer, tumour microenvironmentShi RWang ZZhang JYu ZAn LWei SFeng DWang HDove Medical Pressarticlen6-methylandenosinelong noncoding rnasendometrial cancertumour microenvironmentMedicine (General)R5-920ENInternational Journal of General Medicine, Vol Volume 14, Pp 8249-8262 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	n6-methylandenosine long noncoding rnas endometrial cancer tumour microenvironment Medicine (General) R5-920
spellingShingle	n6-methylandenosine long noncoding rnas endometrial cancer tumour microenvironment Medicine (General) R5-920 Shi R Wang Z Zhang J Yu Z An L Wei S Feng D Wang H N6-Methyladenosine-Related Long Noncoding RNAs as Potential Prognosis Biomarkers for Endometrial Cancer
description	Rui Shi, Ziwei Wang, Jun Zhang, Zhicheng Yu, Lanfen An, Sitian Wei, Dilu Feng, Hongbo Wang Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430022, People’s Republic of ChinaCorrespondence: Dilu Feng; Hongbo WangDepartment of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430022, People’s Republic of ChinaTel +86 027 85350583Fax +86 278 572 6362Email fengdilu@126.com; hb_wang1969@sina.comPurpose: Endometrial cancer (EC) is a common gynaecologic malignancy with an increasing incidence rate and mortality in recent years. N6-methylandenosine (m6A)-related long noncoding RNA (lncRNA) plays a vital role in EC, emerging as one of the most abundant RNA modifications.Materials and Methods: The Cancer Genome Atlas (TCGA) database and UCSC Xena were used to download data related to EC. Survival and univariate and multifactorial prognostic analyses were performed for m6A-related lncRNAs. The expression levels of the three lncRNAs were verified using q-PCR. A nomogram was used to create a clinical tool to assess overall survival. To investigate the relationship between m6A-related lncRNA and EC, we downloaded differential genes related to EC from the TCGA database and mined three m6A-related lncRNAs, namely SCARNA9, TRAF3IP2-AS1, and AL133243.2. The data were categorized into high- and low-risk groups based on m6A-associated lncRNA.Results: Survival analysis revealed that the high-risk group had a lower survival rate. Survival analysis of three m6A-associated lncRNAs revealed that cases with high expression of SCARNA9 tended to have a poorer prognosis, whereas the opposite was true for TRAF3IP2-AS1, AL133243.2. Univariate and multifactorial prognostic analyses suggested statistical differences in patients’ age, FIGO stage, pathological grade, risk score, and prognosis of EC, which was confirmed by results of the separate prognostic factor analysis for the three lncRNAs. Risk status was validated as an independent prognostic indicator, and the prognostic nomogram combined patient age, pathological stage, and FIGO classification to assess 3– 5-year survival. Cases from high- and low-risk groups were analysed for the tumour microenvironment and immune cell scores, and stromal cell scores were found to be lower in the high-risk group. Correlations were analysed using different databases for immune cell classification.Conclusion: m6A-related lncRNAs may play a key role in the diagnosis and treatment of EC as targets of prognosis and the immune microenvironment.Keywords: N6-methylandenosine, long noncoding RNAs, endometrial cancer, tumour microenvironment
format	article
author	Shi R Wang Z Zhang J Yu Z An L Wei S Feng D Wang H
author_facet	Shi R Wang Z Zhang J Yu Z An L Wei S Feng D Wang H
author_sort	Shi R
title	N6-Methyladenosine-Related Long Noncoding RNAs as Potential Prognosis Biomarkers for Endometrial Cancer
title_short	N6-Methyladenosine-Related Long Noncoding RNAs as Potential Prognosis Biomarkers for Endometrial Cancer
title_full	N6-Methyladenosine-Related Long Noncoding RNAs as Potential Prognosis Biomarkers for Endometrial Cancer
title_fullStr	N6-Methyladenosine-Related Long Noncoding RNAs as Potential Prognosis Biomarkers for Endometrial Cancer
title_full_unstemmed	N6-Methyladenosine-Related Long Noncoding RNAs as Potential Prognosis Biomarkers for Endometrial Cancer
title_sort	n6-methyladenosine-related long noncoding rnas as potential prognosis biomarkers for endometrial cancer
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/9c8aa7dfe9c748359b029735fde8e2e4
work_keys_str_mv	AT shir n6methyladenosinerelatedlongnoncodingrnasaspotentialprognosisbiomarkersforendometrialcancer AT wangz n6methyladenosinerelatedlongnoncodingrnasaspotentialprognosisbiomarkersforendometrialcancer AT zhangj n6methyladenosinerelatedlongnoncodingrnasaspotentialprognosisbiomarkersforendometrialcancer AT yuz n6methyladenosinerelatedlongnoncodingrnasaspotentialprognosisbiomarkersforendometrialcancer AT anl n6methyladenosinerelatedlongnoncodingrnasaspotentialprognosisbiomarkersforendometrialcancer AT weis n6methyladenosinerelatedlongnoncodingrnasaspotentialprognosisbiomarkersforendometrialcancer AT fengd n6methyladenosinerelatedlongnoncodingrnasaspotentialprognosisbiomarkersforendometrialcancer AT wangh n6methyladenosinerelatedlongnoncodingrnasaspotentialprognosisbiomarkersforendometrialcancer
_version_	1718426257550475264

N6-Methyladenosine-Related Long Noncoding RNAs as Potential Prognosis Biomarkers for Endometrial Cancer

Ejemplares similares