Extraction and Encapsulation of Phenolic Compounds of Tunisian Rosemary (<i>Rosmarinus officinalis</i> L.) Extracts in Silk Fibroin Nanoparticles

Rosemary (<i>Rosmarinus officinalis</i> L.) is known to be an effective potential source of natural antioxidants which confer benefits to human health. Their bioactive properties are mainly due to phenolic compounds but these molecules are highly vulnerable to oxidants, light, heat, pH,...

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Autores principales:	Kheiria Hcini, Antonio A. Lozano-Pérez, José Luis Cenis, María Quílez, Maria José Jordán
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Rosmarinus officinalis</i> L. extracts post-distilled residues HPLC radical scavenging activity DPPH silk fibroin nanoparticles (SFNs) Botany QK1-989
Acceso en línea:	https://doaj.org/article/9c928217300b476ab0841d6a81336285
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spelling	oai:doaj.org-article:9c928217300b476ab0841d6a813362852021-11-25T18:45:12ZExtraction and Encapsulation of Phenolic Compounds of Tunisian Rosemary (<i>Rosmarinus officinalis</i> L.) Extracts in Silk Fibroin Nanoparticles10.3390/plants101123122223-7747https://doaj.org/article/9c928217300b476ab0841d6a813362852021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2312https://doaj.org/toc/2223-7747Rosemary (<i>Rosmarinus officinalis</i> L.) is known to be an effective potential source of natural antioxidants which confer benefits to human health. Their bioactive properties are mainly due to phenolic compounds but these molecules are highly vulnerable to oxidants, light, heat, pH, water and enzymatic activities. Therefore, the stability and shelf life of phenolic compounds should be increased by being protected from chemical and physical damage by means of encapsulation prior to application. Encapsulation is becoming increasingly important in the pharmaceutical, food, cosmetics, textile, personal care, chemical, biotechnology, and medicinal industries due to its potential for stabilization and delivery of delicate and precious bioactive compounds. The aim of the present work was to describe the polyphenolic profile of Tunisian Rosemary, collected from two different bioclimatic areas, and further loading in silk fibroin nanoparticles. The loaded nanoparticles were characterized in terms of morphology, size, polydispersity, Z-potential, secondary structure of the protein, encapsulation efficiency, loading content, and antioxidant activity. On one hand, HPLC analysis revealed the presence of 18 polyphenolic compounds of whichcarnosic acid and carnosol were found to be the most abundant compounds (46.3 to 76.4 and 22.4 to 43.5 mg of compound per gram of dry plant weight (mg/g DPW) respectively), Total phenolic content (TPC) ranged from 85.8 to 137.3 mg of gallic acid equivalent (GAE)/g DPW in post-distilled rosemary extracts andantioxidant activity reached the values of 5.9 to 8.3 µmol of ascorbic acid equivalent (AAE)/g DPW). On the other hand loaded nanoparticles were almost spherical and presented nanometric size and negative Z-potential. Although the encapsulation efficiency in silk fibroin nanoparticles and the drug loading content were low in the conditions of the assay, the encapsulated polyphenols retained near 85% of the radical scavenging activity against DPPH· after 24 h. of incubation at 37 °C. The results showed that post-distilled rosemary residues had an effective potential as natural antioxidants due to their significant antioxidant activity and seemed to be useful in both pharmaceutical and food industries with beneficial properties that might confer benefits to human health and these silk fibroin nanoparticles loaded with rosemary extracts are thus a promising combination for several applications in food technology or nanomedicine.Kheiria HciniAntonio A. Lozano-PérezJosé Luis CenisMaría QuílezMaria José JordánMDPI AGarticle<i>Rosmarinus officinalis</i> L. extractspost-distilled residuesHPLCradical scavenging activityDPPHsilk fibroin nanoparticles (SFNs)BotanyQK1-989ENPlants, Vol 10, Iss 2312, p 2312 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Rosmarinus officinalis</i> L. extracts post-distilled residues HPLC radical scavenging activity DPPH silk fibroin nanoparticles (SFNs) Botany QK1-989
spellingShingle	<i>Rosmarinus officinalis</i> L. extracts post-distilled residues HPLC radical scavenging activity DPPH silk fibroin nanoparticles (SFNs) Botany QK1-989 Kheiria Hcini Antonio A. Lozano-Pérez José Luis Cenis María Quílez Maria José Jordán Extraction and Encapsulation of Phenolic Compounds of Tunisian Rosemary (<i>Rosmarinus officinalis</i> L.) Extracts in Silk Fibroin Nanoparticles
description	Rosemary (<i>Rosmarinus officinalis</i> L.) is known to be an effective potential source of natural antioxidants which confer benefits to human health. Their bioactive properties are mainly due to phenolic compounds but these molecules are highly vulnerable to oxidants, light, heat, pH, water and enzymatic activities. Therefore, the stability and shelf life of phenolic compounds should be increased by being protected from chemical and physical damage by means of encapsulation prior to application. Encapsulation is becoming increasingly important in the pharmaceutical, food, cosmetics, textile, personal care, chemical, biotechnology, and medicinal industries due to its potential for stabilization and delivery of delicate and precious bioactive compounds. The aim of the present work was to describe the polyphenolic profile of Tunisian Rosemary, collected from two different bioclimatic areas, and further loading in silk fibroin nanoparticles. The loaded nanoparticles were characterized in terms of morphology, size, polydispersity, Z-potential, secondary structure of the protein, encapsulation efficiency, loading content, and antioxidant activity. On one hand, HPLC analysis revealed the presence of 18 polyphenolic compounds of whichcarnosic acid and carnosol were found to be the most abundant compounds (46.3 to 76.4 and 22.4 to 43.5 mg of compound per gram of dry plant weight (mg/g DPW) respectively), Total phenolic content (TPC) ranged from 85.8 to 137.3 mg of gallic acid equivalent (GAE)/g DPW in post-distilled rosemary extracts andantioxidant activity reached the values of 5.9 to 8.3 µmol of ascorbic acid equivalent (AAE)/g DPW). On the other hand loaded nanoparticles were almost spherical and presented nanometric size and negative Z-potential. Although the encapsulation efficiency in silk fibroin nanoparticles and the drug loading content were low in the conditions of the assay, the encapsulated polyphenols retained near 85% of the radical scavenging activity against DPPH· after 24 h. of incubation at 37 °C. The results showed that post-distilled rosemary residues had an effective potential as natural antioxidants due to their significant antioxidant activity and seemed to be useful in both pharmaceutical and food industries with beneficial properties that might confer benefits to human health and these silk fibroin nanoparticles loaded with rosemary extracts are thus a promising combination for several applications in food technology or nanomedicine.
format	article
author	Kheiria Hcini Antonio A. Lozano-Pérez José Luis Cenis María Quílez Maria José Jordán
author_facet	Kheiria Hcini Antonio A. Lozano-Pérez José Luis Cenis María Quílez Maria José Jordán
author_sort	Kheiria Hcini
title	Extraction and Encapsulation of Phenolic Compounds of Tunisian Rosemary (<i>Rosmarinus officinalis</i> L.) Extracts in Silk Fibroin Nanoparticles
title_short	Extraction and Encapsulation of Phenolic Compounds of Tunisian Rosemary (<i>Rosmarinus officinalis</i> L.) Extracts in Silk Fibroin Nanoparticles
title_full	Extraction and Encapsulation of Phenolic Compounds of Tunisian Rosemary (<i>Rosmarinus officinalis</i> L.) Extracts in Silk Fibroin Nanoparticles
title_fullStr	Extraction and Encapsulation of Phenolic Compounds of Tunisian Rosemary (<i>Rosmarinus officinalis</i> L.) Extracts in Silk Fibroin Nanoparticles
title_full_unstemmed	Extraction and Encapsulation of Phenolic Compounds of Tunisian Rosemary (<i>Rosmarinus officinalis</i> L.) Extracts in Silk Fibroin Nanoparticles
title_sort	extraction and encapsulation of phenolic compounds of tunisian rosemary (<i>rosmarinus officinalis</i> l.) extracts in silk fibroin nanoparticles
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9c928217300b476ab0841d6a81336285
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Extraction and Encapsulation of Phenolic Compounds of Tunisian Rosemary (<i>Rosmarinus officinalis</i> L.) Extracts in Silk Fibroin Nanoparticles

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