Tyrosol, at the Concentration Found in Maltese Extra Virgin Olive Oil, Induces HL-60 Differentiation towards the Monocyte lineage

Tyrosol is a phenolic found in extra virgin olive oil (EVOO). In a Maltese monocultivar EVOO, it was present at a concentration of 9.23 ppm. The HL-60 acute myeloid leukaemia cell line, which can be differentiated to both monocytes and neutrophils, was exposed to tyrosol at this concentration and an...

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Autores principales:	Lucienne Gatt, David G. Saliba, Pierre Schembri-Wismayer, Marion Zammit-Mangion
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	HL-60 phenols differentiation apoptosis transcriptome Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/9c9c5eaf81964ebf960bcd33eabe9a42
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spelling	oai:doaj.org-article:9c9c5eaf81964ebf960bcd33eabe9a422021-11-11T15:15:22ZTyrosol, at the Concentration Found in Maltese Extra Virgin Olive Oil, Induces HL-60 Differentiation towards the Monocyte lineage10.3390/app1121101992076-3417https://doaj.org/article/9c9c5eaf81964ebf960bcd33eabe9a422021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/21/10199https://doaj.org/toc/2076-3417Tyrosol is a phenolic found in extra virgin olive oil (EVOO). In a Maltese monocultivar EVOO, it was present at a concentration of 9.23 ppm. The HL-60 acute myeloid leukaemia cell line, which can be differentiated to both monocytes and neutrophils, was exposed to tyrosol at this concentration and analysed for evidence of differentiation and effects of cytotoxicity. The polyphenol induced a 1.93-fold increase in cellular oxidative activity (<i>p</i>-value 0.044) and enhanced surface expression of CD11b and CD14. This indicates that tyrosol induces monocytic-like differentiation. An RNA-seq analysis confirmed the upregulation of monocyte genes and the loss of neutrophil genes concomitant with the bi-potential promyelocyte precursor moving down the monocytic pathway. A cell cycle analysis showed an accumulation of cells in the Sub G<sub>0</sub>/G<sub>1</sub> phase following tyrosol exposure for 5 days, which coincided with an increase in apoptotic and necrotic markers. This indicates differentiation followed by cell death, unlike the positive monocyte differentiation control PMA. This selective cytotoxic effect following differentiation indicates therapeutic potential against leukaemia.Lucienne GattDavid G. SalibaPierre Schembri-WismayerMarion Zammit-MangionMDPI AGarticleHL-60phenolsdifferentiationapoptosistranscriptomeTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 10199, p 10199 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HL-60 phenols differentiation apoptosis transcriptome Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	HL-60 phenols differentiation apoptosis transcriptome Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Lucienne Gatt David G. Saliba Pierre Schembri-Wismayer Marion Zammit-Mangion Tyrosol, at the Concentration Found in Maltese Extra Virgin Olive Oil, Induces HL-60 Differentiation towards the Monocyte lineage
description	Tyrosol is a phenolic found in extra virgin olive oil (EVOO). In a Maltese monocultivar EVOO, it was present at a concentration of 9.23 ppm. The HL-60 acute myeloid leukaemia cell line, which can be differentiated to both monocytes and neutrophils, was exposed to tyrosol at this concentration and analysed for evidence of differentiation and effects of cytotoxicity. The polyphenol induced a 1.93-fold increase in cellular oxidative activity (<i>p</i>-value 0.044) and enhanced surface expression of CD11b and CD14. This indicates that tyrosol induces monocytic-like differentiation. An RNA-seq analysis confirmed the upregulation of monocyte genes and the loss of neutrophil genes concomitant with the bi-potential promyelocyte precursor moving down the monocytic pathway. A cell cycle analysis showed an accumulation of cells in the Sub G<sub>0</sub>/G<sub>1</sub> phase following tyrosol exposure for 5 days, which coincided with an increase in apoptotic and necrotic markers. This indicates differentiation followed by cell death, unlike the positive monocyte differentiation control PMA. This selective cytotoxic effect following differentiation indicates therapeutic potential against leukaemia.
format	article
author	Lucienne Gatt David G. Saliba Pierre Schembri-Wismayer Marion Zammit-Mangion
author_facet	Lucienne Gatt David G. Saliba Pierre Schembri-Wismayer Marion Zammit-Mangion
author_sort	Lucienne Gatt
title	Tyrosol, at the Concentration Found in Maltese Extra Virgin Olive Oil, Induces HL-60 Differentiation towards the Monocyte lineage
title_short	Tyrosol, at the Concentration Found in Maltese Extra Virgin Olive Oil, Induces HL-60 Differentiation towards the Monocyte lineage
title_full	Tyrosol, at the Concentration Found in Maltese Extra Virgin Olive Oil, Induces HL-60 Differentiation towards the Monocyte lineage
title_fullStr	Tyrosol, at the Concentration Found in Maltese Extra Virgin Olive Oil, Induces HL-60 Differentiation towards the Monocyte lineage
title_full_unstemmed	Tyrosol, at the Concentration Found in Maltese Extra Virgin Olive Oil, Induces HL-60 Differentiation towards the Monocyte lineage
title_sort	tyrosol, at the concentration found in maltese extra virgin olive oil, induces hl-60 differentiation towards the monocyte lineage
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9c9c5eaf81964ebf960bcd33eabe9a42
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Tyrosol, at the Concentration Found in Maltese Extra Virgin Olive Oil, Induces HL-60 Differentiation towards the Monocyte lineage

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