Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9

CRISPR-Cas9 has been used to image repetitive loci in live cells to explore genome organization. Here the authors demonstrate the imaging of low-repeat regions with a single-guide RNA and the imaging of a non-repetitive region, allowing the tracking of transcriptionally active and inactive regions.

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Autores principales:	Peiwu Qin, Mahmut Parlak, Cem Kuscu, Jigar Bandaria, Mustafa Mir, Karol Szlachta, Ritambhara Singh, Xavier Darzacq, Ahmet Yildiz, Mazhar Adli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/9c9f9c3c40e54de893c731a5ffb04a1a
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spelling	oai:doaj.org-article:9c9f9c3c40e54de893c731a5ffb04a1a2021-12-02T14:41:21ZLive cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas910.1038/ncomms147252041-1723https://doaj.org/article/9c9f9c3c40e54de893c731a5ffb04a1a2017-03-01T00:00:00Zhttps://doi.org/10.1038/ncomms14725https://doaj.org/toc/2041-1723CRISPR-Cas9 has been used to image repetitive loci in live cells to explore genome organization. Here the authors demonstrate the imaging of low-repeat regions with a single-guide RNA and the imaging of a non-repetitive region, allowing the tracking of transcriptionally active and inactive regions.Peiwu QinMahmut ParlakCem KuscuJigar BandariaMustafa MirKarol SzlachtaRitambhara SinghXavier DarzacqAhmet YildizMazhar AdliNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Peiwu Qin Mahmut Parlak Cem Kuscu Jigar Bandaria Mustafa Mir Karol Szlachta Ritambhara Singh Xavier Darzacq Ahmet Yildiz Mazhar Adli Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9
description	CRISPR-Cas9 has been used to image repetitive loci in live cells to explore genome organization. Here the authors demonstrate the imaging of low-repeat regions with a single-guide RNA and the imaging of a non-repetitive region, allowing the tracking of transcriptionally active and inactive regions.
format	article
author	Peiwu Qin Mahmut Parlak Cem Kuscu Jigar Bandaria Mustafa Mir Karol Szlachta Ritambhara Singh Xavier Darzacq Ahmet Yildiz Mazhar Adli
author_facet	Peiwu Qin Mahmut Parlak Cem Kuscu Jigar Bandaria Mustafa Mir Karol Szlachta Ritambhara Singh Xavier Darzacq Ahmet Yildiz Mazhar Adli
author_sort	Peiwu Qin
title	Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9
title_short	Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9
title_full	Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9
title_fullStr	Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9
title_full_unstemmed	Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9
title_sort	live cell imaging of low- and non-repetitive chromosome loci using crispr-cas9
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9c9f9c3c40e54de893c731a5ffb04a1a
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Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9

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