Intraoperative imaging of hepatic cancers using γ-glutamyltranspeptidase-specific fluorophore enabling real-time identification and estimation of recurrence

Abstract γ-Glutamyltranspeptidase (GGT) is upregulated in a variety of human cancers including primary and secondary hepatic tumors. This motivated us to use γ-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG), a novel fluorophore emitting light at around 520 nm following enzymatic reaction with GG...

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Autores principales:	Yoichi Miyata, Takeaki Ishizawa, Mako Kamiya, Suguru Yamashita, Kiyoshi Hasegawa, Aya Ushiku, Junji Shibahara, Masashi Fukayama, Yasuteru Urano, Norihiro Kokudo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9cb4d92055d14ee191258d9fb94d6e7a
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spelling	oai:doaj.org-article:9cb4d92055d14ee191258d9fb94d6e7a2021-12-02T11:52:30ZIntraoperative imaging of hepatic cancers using γ-glutamyltranspeptidase-specific fluorophore enabling real-time identification and estimation of recurrence10.1038/s41598-017-03760-32045-2322https://doaj.org/article/9cb4d92055d14ee191258d9fb94d6e7a2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03760-3https://doaj.org/toc/2045-2322Abstract γ-Glutamyltranspeptidase (GGT) is upregulated in a variety of human cancers including primary and secondary hepatic tumors. This motivated us to use γ-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG), a novel fluorophore emitting light at around 520 nm following enzymatic reaction with GGT, as a tool for the intraoperative identification of hepatic tumors. gGlu-HMRG was topically applied to 103 freshly resected hepatic specimens. Fluorescence imaging using gGlu-HMRG identified hepatic tumors with the sensitivity/specificity of 48%/96% for hepatocellular carcinoma, 100%/100% for intrahepatic cholangiocarcinoma, and 87%/100% for colorectal liver metastasis. High gGlu-HMRG fluorescence intensity was positively associated with the incidence of microscopic vascular invasion in HCC and the risk of early postoperative recurrence in CRLM. These results suggest that gGlu-HMRG imaging could not only be a useful intraoperative navigation tool but also provide information related to postoperative disease recurrence.Yoichi MiyataTakeaki IshizawaMako KamiyaSuguru YamashitaKiyoshi HasegawaAya UshikuJunji ShibaharaMasashi FukayamaYasuteru UranoNorihiro KokudoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yoichi Miyata Takeaki Ishizawa Mako Kamiya Suguru Yamashita Kiyoshi Hasegawa Aya Ushiku Junji Shibahara Masashi Fukayama Yasuteru Urano Norihiro Kokudo Intraoperative imaging of hepatic cancers using γ-glutamyltranspeptidase-specific fluorophore enabling real-time identification and estimation of recurrence
description	Abstract γ-Glutamyltranspeptidase (GGT) is upregulated in a variety of human cancers including primary and secondary hepatic tumors. This motivated us to use γ-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG), a novel fluorophore emitting light at around 520 nm following enzymatic reaction with GGT, as a tool for the intraoperative identification of hepatic tumors. gGlu-HMRG was topically applied to 103 freshly resected hepatic specimens. Fluorescence imaging using gGlu-HMRG identified hepatic tumors with the sensitivity/specificity of 48%/96% for hepatocellular carcinoma, 100%/100% for intrahepatic cholangiocarcinoma, and 87%/100% for colorectal liver metastasis. High gGlu-HMRG fluorescence intensity was positively associated with the incidence of microscopic vascular invasion in HCC and the risk of early postoperative recurrence in CRLM. These results suggest that gGlu-HMRG imaging could not only be a useful intraoperative navigation tool but also provide information related to postoperative disease recurrence.
format	article
author	Yoichi Miyata Takeaki Ishizawa Mako Kamiya Suguru Yamashita Kiyoshi Hasegawa Aya Ushiku Junji Shibahara Masashi Fukayama Yasuteru Urano Norihiro Kokudo
author_facet	Yoichi Miyata Takeaki Ishizawa Mako Kamiya Suguru Yamashita Kiyoshi Hasegawa Aya Ushiku Junji Shibahara Masashi Fukayama Yasuteru Urano Norihiro Kokudo
author_sort	Yoichi Miyata
title	Intraoperative imaging of hepatic cancers using γ-glutamyltranspeptidase-specific fluorophore enabling real-time identification and estimation of recurrence
title_short	Intraoperative imaging of hepatic cancers using γ-glutamyltranspeptidase-specific fluorophore enabling real-time identification and estimation of recurrence
title_full	Intraoperative imaging of hepatic cancers using γ-glutamyltranspeptidase-specific fluorophore enabling real-time identification and estimation of recurrence
title_fullStr	Intraoperative imaging of hepatic cancers using γ-glutamyltranspeptidase-specific fluorophore enabling real-time identification and estimation of recurrence
title_full_unstemmed	Intraoperative imaging of hepatic cancers using γ-glutamyltranspeptidase-specific fluorophore enabling real-time identification and estimation of recurrence
title_sort	intraoperative imaging of hepatic cancers using γ-glutamyltranspeptidase-specific fluorophore enabling real-time identification and estimation of recurrence
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9cb4d92055d14ee191258d9fb94d6e7a
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Intraoperative imaging of hepatic cancers using γ-glutamyltranspeptidase-specific fluorophore enabling real-time identification and estimation of recurrence

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