Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System

Lipoic acid (LA, 6,8-dithiooctanoic acid) is a sulfur containing coenzyme essential for the activity of several key enzymes involved in oxidative and single carbon metabolism in most bacteria and eukaryotes. LA is synthetized by the concerted activity of the octanoyltransferase (LIP2, EC 2.3.1.181)...

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Autores principales:	Raquel Martins-Noguerol, Sébastien Acket, M. Adrián Troncoso-Ponce, Rafael Garcés, Brigitte Thomasset, Mónica Venegas-Calerón, Joaquín J. Salas, Enrique Martínez-Force, Antonio J. Moreno-Pérez
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	sunflower octanoyltransferase lipoyl synthase lipoic acid mitochondrial lipoylation Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/9cb6d1819eda42d1b3ce87e66c47bd76
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spelling	oai:doaj.org-article:9cb6d1819eda42d1b3ce87e66c47bd762021-11-18T09:05:41ZCharacterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System1664-462X10.3389/fpls.2021.781917https://doaj.org/article/9cb6d1819eda42d1b3ce87e66c47bd762021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.781917/fullhttps://doaj.org/toc/1664-462XLipoic acid (LA, 6,8-dithiooctanoic acid) is a sulfur containing coenzyme essential for the activity of several key enzymes involved in oxidative and single carbon metabolism in most bacteria and eukaryotes. LA is synthetized by the concerted activity of the octanoyltransferase (LIP2, EC 2.3.1.181) and lipoyl synthase (LIP1, EC 2.8.1.8) enzymes. In plants, pyruvate dehydrogenase (PDH), 2-oxoglutarate dehydrogenase or glycine decarboxylase are essential complexes that need to be lipoylated. These lipoylated enzymes and complexes are located in the mitochondria, while PDH is also present in plastids where it provides acetyl-CoA for de novo fatty acid biosynthesis. As such, lipoylation of PDH could regulate fatty acid synthesis in both these organelles. In the present work, the sunflower LIP1 and LIP2 genes (HaLIP1m and HaLIP2m) were isolated sequenced, cloned, and characterized, evaluating their putative mitochondrial location. The expression of these genes was studied in different tissues and protein docking was modeled. The genes were also expressed in Escherichia coli and Arabidopsis thaliana, where their impact on fatty acid and glycerolipid composition was assessed. Lipidomic studies in Arabidopsis revealed lipid remodeling in lines overexpressing these enzymes and the involvement of both sunflower proteins in the phenotypes observed is discussed in the light of the results obtained.Raquel Martins-NoguerolSébastien AcketM. Adrián Troncoso-PonceRafael GarcésBrigitte ThomassetMónica Venegas-CalerónJoaquín J. SalasEnrique Martínez-ForceAntonio J. Moreno-PérezFrontiers Media S.A.articlesunfloweroctanoyltransferaselipoyl synthaselipoic acidmitochondrial lipoylationPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	sunflower octanoyltransferase lipoyl synthase lipoic acid mitochondrial lipoylation Plant culture SB1-1110
spellingShingle	sunflower octanoyltransferase lipoyl synthase lipoic acid mitochondrial lipoylation Plant culture SB1-1110 Raquel Martins-Noguerol Sébastien Acket M. Adrián Troncoso-Ponce Rafael Garcés Brigitte Thomasset Mónica Venegas-Calerón Joaquín J. Salas Enrique Martínez-Force Antonio J. Moreno-Pérez Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System
description	Lipoic acid (LA, 6,8-dithiooctanoic acid) is a sulfur containing coenzyme essential for the activity of several key enzymes involved in oxidative and single carbon metabolism in most bacteria and eukaryotes. LA is synthetized by the concerted activity of the octanoyltransferase (LIP2, EC 2.3.1.181) and lipoyl synthase (LIP1, EC 2.8.1.8) enzymes. In plants, pyruvate dehydrogenase (PDH), 2-oxoglutarate dehydrogenase or glycine decarboxylase are essential complexes that need to be lipoylated. These lipoylated enzymes and complexes are located in the mitochondria, while PDH is also present in plastids where it provides acetyl-CoA for de novo fatty acid biosynthesis. As such, lipoylation of PDH could regulate fatty acid synthesis in both these organelles. In the present work, the sunflower LIP1 and LIP2 genes (HaLIP1m and HaLIP2m) were isolated sequenced, cloned, and characterized, evaluating their putative mitochondrial location. The expression of these genes was studied in different tissues and protein docking was modeled. The genes were also expressed in Escherichia coli and Arabidopsis thaliana, where their impact on fatty acid and glycerolipid composition was assessed. Lipidomic studies in Arabidopsis revealed lipid remodeling in lines overexpressing these enzymes and the involvement of both sunflower proteins in the phenotypes observed is discussed in the light of the results obtained.
format	article
author	Raquel Martins-Noguerol Sébastien Acket M. Adrián Troncoso-Ponce Rafael Garcés Brigitte Thomasset Mónica Venegas-Calerón Joaquín J. Salas Enrique Martínez-Force Antonio J. Moreno-Pérez
author_facet	Raquel Martins-Noguerol Sébastien Acket M. Adrián Troncoso-Ponce Rafael Garcés Brigitte Thomasset Mónica Venegas-Calerón Joaquín J. Salas Enrique Martínez-Force Antonio J. Moreno-Pérez
author_sort	Raquel Martins-Noguerol
title	Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System
title_short	Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System
title_full	Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System
title_fullStr	Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System
title_full_unstemmed	Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System
title_sort	characterization of helianthus annuus lipoic acid biosynthesis: the mitochondrial octanoyltransferase and lipoyl synthase enzyme system
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/9cb6d1819eda42d1b3ce87e66c47bd76
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Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System

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