Response of Lactobacillus plantarum VAL6 to challenges of pH and sodium chloride stresses

Abstract To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride...

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Autores principales:	Phu-Tho Nguyen, Thi-Tho Nguyen, Thi-Ngoc-Tuyen Vo, Thi-Thanh-Xuan Nguyen, Quoc-Khanh Hoang, Huu-Thanh Nguyen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9cb84b86e9b34765a219be3821a74a8e
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spelling	oai:doaj.org-article:9cb84b86e9b34765a219be3821a74a8e2021-12-02T15:23:00ZResponse of Lactobacillus plantarum VAL6 to challenges of pH and sodium chloride stresses10.1038/s41598-020-80634-12045-2322https://doaj.org/article/9cb84b86e9b34765a219be3821a74a8e2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80634-1https://doaj.org/toc/2045-2322Abstract To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments. The results found that Lactobacillus plantarum VAL6 exposed to stress at pH 3 for 3 h gives the highest exopolysaccharide yield (50.44 g/L) which is 6.4 fold higher than non-stress. Under pH and sodium chloride stresses, the mannose content in exopolysaccharides decreased while the glucose increased in comparison with non-stress condition. The galactose content was highest under stress condition of pH 8 meantime rhamnose content increased sharply when Lactobacillus plantarum VAL6 was stressed at pH 3. The arabinose content in exopolysaccharides was not detected under non-stress condition but it was recorded in great amounts after 3 h of stress at pH 3. In addition, stress of pH 8 triggered the mRNA expression of epsF gene resulting in galactose-rich EPS synthesis. According to our results, the stresses of pH and sodium chloride enhance the production and change the mRNA expression of epsF gene, leading to differences in the monosaccharide composition of exopolysaccharides.Phu-Tho NguyenThi-Tho NguyenThi-Ngoc-Tuyen VoThi-Thanh-Xuan NguyenQuoc-Khanh HoangHuu-Thanh NguyenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Phu-Tho Nguyen Thi-Tho Nguyen Thi-Ngoc-Tuyen Vo Thi-Thanh-Xuan Nguyen Quoc-Khanh Hoang Huu-Thanh Nguyen Response of Lactobacillus plantarum VAL6 to challenges of pH and sodium chloride stresses
description	Abstract To investigate the effect of environmental stresses on the exopolysaccharide biosynthesis, after 24 h of culture at 37 °C with pH 6.8 and without sodium chloride, Lactobacillus plantarum VAL6 was exposed to different stress conditions, including pH (pHs of 3 and 8) and high sodium chloride concentration treatments. The results found that Lactobacillus plantarum VAL6 exposed to stress at pH 3 for 3 h gives the highest exopolysaccharide yield (50.44 g/L) which is 6.4 fold higher than non-stress. Under pH and sodium chloride stresses, the mannose content in exopolysaccharides decreased while the glucose increased in comparison with non-stress condition. The galactose content was highest under stress condition of pH 8 meantime rhamnose content increased sharply when Lactobacillus plantarum VAL6 was stressed at pH 3. The arabinose content in exopolysaccharides was not detected under non-stress condition but it was recorded in great amounts after 3 h of stress at pH 3. In addition, stress of pH 8 triggered the mRNA expression of epsF gene resulting in galactose-rich EPS synthesis. According to our results, the stresses of pH and sodium chloride enhance the production and change the mRNA expression of epsF gene, leading to differences in the monosaccharide composition of exopolysaccharides.
format	article
author	Phu-Tho Nguyen Thi-Tho Nguyen Thi-Ngoc-Tuyen Vo Thi-Thanh-Xuan Nguyen Quoc-Khanh Hoang Huu-Thanh Nguyen
author_facet	Phu-Tho Nguyen Thi-Tho Nguyen Thi-Ngoc-Tuyen Vo Thi-Thanh-Xuan Nguyen Quoc-Khanh Hoang Huu-Thanh Nguyen
author_sort	Phu-Tho Nguyen
title	Response of Lactobacillus plantarum VAL6 to challenges of pH and sodium chloride stresses
title_short	Response of Lactobacillus plantarum VAL6 to challenges of pH and sodium chloride stresses
title_full	Response of Lactobacillus plantarum VAL6 to challenges of pH and sodium chloride stresses
title_fullStr	Response of Lactobacillus plantarum VAL6 to challenges of pH and sodium chloride stresses
title_full_unstemmed	Response of Lactobacillus plantarum VAL6 to challenges of pH and sodium chloride stresses
title_sort	response of lactobacillus plantarum val6 to challenges of ph and sodium chloride stresses
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9cb84b86e9b34765a219be3821a74a8e
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Response of Lactobacillus plantarum VAL6 to challenges of pH and sodium chloride stresses

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