Changes in the Eye Microbiota Associated with Contact Lens Wearing

ABSTRACT Wearing contact lenses has been identified as a risk factor for the development of eye conditions such as giant papillary conjunctivitis and keratitis. We hypothesized that wearing contact lenses is associated with changes in the ocular microbiota. We compared the bacterial communities of t...

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Autores principales:	Hakdong Shin, Kenneth Price, Luong Albert, Jack Dodick, Lisa Park, Maria Gloria Dominguez-Bello
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/9cb94d59115c4b50a3fd75783ca5d879
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spelling	oai:doaj.org-article:9cb94d59115c4b50a3fd75783ca5d8792021-11-15T15:41:41ZChanges in the Eye Microbiota Associated with Contact Lens Wearing10.1128/mBio.00198-162150-7511https://doaj.org/article/9cb94d59115c4b50a3fd75783ca5d8792016-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00198-16https://doaj.org/toc/2150-7511ABSTRACT Wearing contact lenses has been identified as a risk factor for the development of eye conditions such as giant papillary conjunctivitis and keratitis. We hypothesized that wearing contact lenses is associated with changes in the ocular microbiota. We compared the bacterial communities of the conjunctiva and skin under the eye from 58 subjects and analyzed samples from 20 subjects (9 lens wearers and 11 non-lens wearers) taken at 3 time points using a 16S rRNA gene-based sequencing technique (V4 region; Illumina MiSeq). We found that using anesthetic eye drops before sampling decreases the detected ocular microbiota diversity. Compared to those from non-lens wearers, dry conjunctival swabs from lens wearers had more variable and skin-like bacterial community structures (UniFrac; P value = <0.001), with higher abundances of Methylobacterium, Lactobacillus, Acinetobacter, and Pseudomonas and lower abundances of Haemophilus, Streptococcus, Staphylococcus, and Corynebacterium (linear discriminant analysis [LDA] score = >3.0). The results indicate that wearing contact lenses alters the microbial structure of the ocular conjunctiva, making it more similar to that of the skin microbiota. Further research is needed to determine whether the microbiome structure provides less protection from ocular infections. IMPORTANCE As in other body sites (i.e., the gut, skin, and mouth), the eye has a normal community of bacteria which are expected to confer resistance that provides protection from invaders. However, the eye microbiome has been largely neglected and is relevant to eye health and understanding eye diseases and to discovery of its functions. This report of a baseline study shows differences in the eye microbiome of contact lens wearers in relation to those of non-lens wearers and has the potential to help future studies explore novel insights into a possible role of the microbiome in the increased risk for eye infections in contact lens wearers.Hakdong ShinKenneth PriceLuong AlbertJack DodickLisa ParkMaria Gloria Dominguez-BelloAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 7, Iss 2 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Hakdong Shin Kenneth Price Luong Albert Jack Dodick Lisa Park Maria Gloria Dominguez-Bello Changes in the Eye Microbiota Associated with Contact Lens Wearing
description	ABSTRACT Wearing contact lenses has been identified as a risk factor for the development of eye conditions such as giant papillary conjunctivitis and keratitis. We hypothesized that wearing contact lenses is associated with changes in the ocular microbiota. We compared the bacterial communities of the conjunctiva and skin under the eye from 58 subjects and analyzed samples from 20 subjects (9 lens wearers and 11 non-lens wearers) taken at 3 time points using a 16S rRNA gene-based sequencing technique (V4 region; Illumina MiSeq). We found that using anesthetic eye drops before sampling decreases the detected ocular microbiota diversity. Compared to those from non-lens wearers, dry conjunctival swabs from lens wearers had more variable and skin-like bacterial community structures (UniFrac; P value = <0.001), with higher abundances of Methylobacterium, Lactobacillus, Acinetobacter, and Pseudomonas and lower abundances of Haemophilus, Streptococcus, Staphylococcus, and Corynebacterium (linear discriminant analysis [LDA] score = >3.0). The results indicate that wearing contact lenses alters the microbial structure of the ocular conjunctiva, making it more similar to that of the skin microbiota. Further research is needed to determine whether the microbiome structure provides less protection from ocular infections. IMPORTANCE As in other body sites (i.e., the gut, skin, and mouth), the eye has a normal community of bacteria which are expected to confer resistance that provides protection from invaders. However, the eye microbiome has been largely neglected and is relevant to eye health and understanding eye diseases and to discovery of its functions. This report of a baseline study shows differences in the eye microbiome of contact lens wearers in relation to those of non-lens wearers and has the potential to help future studies explore novel insights into a possible role of the microbiome in the increased risk for eye infections in contact lens wearers.
format	article
author	Hakdong Shin Kenneth Price Luong Albert Jack Dodick Lisa Park Maria Gloria Dominguez-Bello
author_facet	Hakdong Shin Kenneth Price Luong Albert Jack Dodick Lisa Park Maria Gloria Dominguez-Bello
author_sort	Hakdong Shin
title	Changes in the Eye Microbiota Associated with Contact Lens Wearing
title_short	Changes in the Eye Microbiota Associated with Contact Lens Wearing
title_full	Changes in the Eye Microbiota Associated with Contact Lens Wearing
title_fullStr	Changes in the Eye Microbiota Associated with Contact Lens Wearing
title_full_unstemmed	Changes in the Eye Microbiota Associated with Contact Lens Wearing
title_sort	changes in the eye microbiota associated with contact lens wearing
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/9cb94d59115c4b50a3fd75783ca5d879
work_keys_str_mv	AT hakdongshin changesintheeyemicrobiotaassociatedwithcontactlenswearing AT kennethprice changesintheeyemicrobiotaassociatedwithcontactlenswearing AT luongalbert changesintheeyemicrobiotaassociatedwithcontactlenswearing AT jackdodick changesintheeyemicrobiotaassociatedwithcontactlenswearing AT lisapark changesintheeyemicrobiotaassociatedwithcontactlenswearing AT mariagloriadominguezbello changesintheeyemicrobiotaassociatedwithcontactlenswearing
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Changes in the Eye Microbiota Associated with Contact Lens Wearing

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