Inferring spikes from calcium imaging in dopamine neurons.

Calcium imaging has led to discoveries about neural correlates of behavior in subcortical neurons, including dopamine (DA) neurons. However, spike inference methods have not been tested in most populations of subcortical neurons. To address this gap, we simultaneously performed calcium imaging and e...

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Autores principales:	Weston Fleming, Sean Jewell, Ben Engelhard, Daniela M Witten, Ilana B Witten
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9cbcfe83b19f424abf6223e9f9530f2c
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spelling	oai:doaj.org-article:9cbcfe83b19f424abf6223e9f9530f2c2021-11-25T06:23:36ZInferring spikes from calcium imaging in dopamine neurons.1932-620310.1371/journal.pone.0252345https://doaj.org/article/9cbcfe83b19f424abf6223e9f9530f2c2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252345https://doaj.org/toc/1932-6203Calcium imaging has led to discoveries about neural correlates of behavior in subcortical neurons, including dopamine (DA) neurons. However, spike inference methods have not been tested in most populations of subcortical neurons. To address this gap, we simultaneously performed calcium imaging and electrophysiology in DA neurons in brain slices and applied a recently developed spike inference algorithm to the GCaMP fluorescence. This revealed that individual spikes can be inferred accurately in this population. Next, we inferred spikes in vivo from calcium imaging from these neurons during Pavlovian conditioning, as well as during navigation in virtual reality. In both cases, we quantitatively recapitulated previous in vivo electrophysiological observations. Our work provides a validated approach to infer spikes from calcium imaging in DA neurons and implies that aspects of both tonic and phasic spike patterns can be recovered.Weston FlemingSean JewellBen EngelhardDaniela M WittenIlana B WittenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0252345 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Weston Fleming Sean Jewell Ben Engelhard Daniela M Witten Ilana B Witten Inferring spikes from calcium imaging in dopamine neurons.
description	Calcium imaging has led to discoveries about neural correlates of behavior in subcortical neurons, including dopamine (DA) neurons. However, spike inference methods have not been tested in most populations of subcortical neurons. To address this gap, we simultaneously performed calcium imaging and electrophysiology in DA neurons in brain slices and applied a recently developed spike inference algorithm to the GCaMP fluorescence. This revealed that individual spikes can be inferred accurately in this population. Next, we inferred spikes in vivo from calcium imaging from these neurons during Pavlovian conditioning, as well as during navigation in virtual reality. In both cases, we quantitatively recapitulated previous in vivo electrophysiological observations. Our work provides a validated approach to infer spikes from calcium imaging in DA neurons and implies that aspects of both tonic and phasic spike patterns can be recovered.
format	article
author	Weston Fleming Sean Jewell Ben Engelhard Daniela M Witten Ilana B Witten
author_facet	Weston Fleming Sean Jewell Ben Engelhard Daniela M Witten Ilana B Witten
author_sort	Weston Fleming
title	Inferring spikes from calcium imaging in dopamine neurons.
title_short	Inferring spikes from calcium imaging in dopamine neurons.
title_full	Inferring spikes from calcium imaging in dopamine neurons.
title_fullStr	Inferring spikes from calcium imaging in dopamine neurons.
title_full_unstemmed	Inferring spikes from calcium imaging in dopamine neurons.
title_sort	inferring spikes from calcium imaging in dopamine neurons.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/9cbcfe83b19f424abf6223e9f9530f2c
work_keys_str_mv	AT westonfleming inferringspikesfromcalciumimagingindopamineneurons AT seanjewell inferringspikesfromcalciumimagingindopamineneurons AT benengelhard inferringspikesfromcalciumimagingindopamineneurons AT danielamwitten inferringspikesfromcalciumimagingindopamineneurons AT ilanabwitten inferringspikesfromcalciumimagingindopamineneurons
_version_	1718413816513953792

Inferring spikes from calcium imaging in dopamine neurons.

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