OSM-9 and an amiloride-sensitive channel, but not PKD-2, are involved in mechanosensation in C. elegans male ray neurons

Abstract Mechanotransduction is crucial for touch sensation, hearing, proprioception, and pain sensing. In C. elegans, male ray neurons have been implicated to be involved in the mechanosensation required for mating behavior. However, whether ray neurons directly sense mechanical stimulation is not...

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Autores principales:	Hu Zhang, Xiaomin Yue, Hankui Cheng, Xiaoyan Zhang, Yang Cai, Wenjuan Zou, Guifang Huang, Lufeng Cheng, Fang Ye, Lijun Kang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9ccab6db3aa84b20ad6ed371370e5080
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spelling	oai:doaj.org-article:9ccab6db3aa84b20ad6ed371370e50802021-12-02T16:08:27ZOSM-9 and an amiloride-sensitive channel, but not PKD-2, are involved in mechanosensation in C. elegans male ray neurons10.1038/s41598-018-25542-12045-2322https://doaj.org/article/9ccab6db3aa84b20ad6ed371370e50802018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25542-1https://doaj.org/toc/2045-2322Abstract Mechanotransduction is crucial for touch sensation, hearing, proprioception, and pain sensing. In C. elegans, male ray neurons have been implicated to be involved in the mechanosensation required for mating behavior. However, whether ray neurons directly sense mechanical stimulation is not yet known, and the underlying molecular mechanisms have not been identified. Using in vivo calcium imaging, we recorded the touch-induced calcium responses in male ray neurons. Our data demonstrated that ray neurons are sensitive to mechanical stimulation in a neurotransmitter-independent manner. PKD-2, a putative sensor component for both mechanosensation and chemosensation in male-specific neurons, was not required for the touch-induced calcium responses in RnB neurons, whereas the TRPV channel OSM-9 shaped the kinetics of the responses. We further showed that RnB-neuron mechanosensation is likely mediated by an amiloride-sensitive DEG/ENaC channel. These observations lay a foundation for better understanding the molecular mechanisms of mechanosensation.Hu ZhangXiaomin YueHankui ChengXiaoyan ZhangYang CaiWenjuan ZouGuifang HuangLufeng ChengFang YeLijun KangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hu Zhang Xiaomin Yue Hankui Cheng Xiaoyan Zhang Yang Cai Wenjuan Zou Guifang Huang Lufeng Cheng Fang Ye Lijun Kang OSM-9 and an amiloride-sensitive channel, but not PKD-2, are involved in mechanosensation in C. elegans male ray neurons
description	Abstract Mechanotransduction is crucial for touch sensation, hearing, proprioception, and pain sensing. In C. elegans, male ray neurons have been implicated to be involved in the mechanosensation required for mating behavior. However, whether ray neurons directly sense mechanical stimulation is not yet known, and the underlying molecular mechanisms have not been identified. Using in vivo calcium imaging, we recorded the touch-induced calcium responses in male ray neurons. Our data demonstrated that ray neurons are sensitive to mechanical stimulation in a neurotransmitter-independent manner. PKD-2, a putative sensor component for both mechanosensation and chemosensation in male-specific neurons, was not required for the touch-induced calcium responses in RnB neurons, whereas the TRPV channel OSM-9 shaped the kinetics of the responses. We further showed that RnB-neuron mechanosensation is likely mediated by an amiloride-sensitive DEG/ENaC channel. These observations lay a foundation for better understanding the molecular mechanisms of mechanosensation.
format	article
author	Hu Zhang Xiaomin Yue Hankui Cheng Xiaoyan Zhang Yang Cai Wenjuan Zou Guifang Huang Lufeng Cheng Fang Ye Lijun Kang
author_facet	Hu Zhang Xiaomin Yue Hankui Cheng Xiaoyan Zhang Yang Cai Wenjuan Zou Guifang Huang Lufeng Cheng Fang Ye Lijun Kang
author_sort	Hu Zhang
title	OSM-9 and an amiloride-sensitive channel, but not PKD-2, are involved in mechanosensation in C. elegans male ray neurons
title_short	OSM-9 and an amiloride-sensitive channel, but not PKD-2, are involved in mechanosensation in C. elegans male ray neurons
title_full	OSM-9 and an amiloride-sensitive channel, but not PKD-2, are involved in mechanosensation in C. elegans male ray neurons
title_fullStr	OSM-9 and an amiloride-sensitive channel, but not PKD-2, are involved in mechanosensation in C. elegans male ray neurons
title_full_unstemmed	OSM-9 and an amiloride-sensitive channel, but not PKD-2, are involved in mechanosensation in C. elegans male ray neurons
title_sort	osm-9 and an amiloride-sensitive channel, but not pkd-2, are involved in mechanosensation in c. elegans male ray neurons
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/9ccab6db3aa84b20ad6ed371370e5080
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OSM-9 and an amiloride-sensitive channel, but not PKD-2, are involved in mechanosensation in C. elegans male ray neurons

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