Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie

Abstract Pigs are susceptible to infection with the classical bovine spongiform encephalopathy (C-BSE) agent following experimental inoculation, and PrPSc accumulation was detected in porcine tissues after the inoculation of certain scrapie and chronic wasting disease isolates. However, a robust tra...

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Autores principales:	Belén Marín, Alicia Otero, Séverine Lugan, Juan Carlos Espinosa, Alba Marín-Moreno, Enric Vidal, Carlos Hedman, Antonio Romero, Martí Pumarola, Juan J. Badiola, Juan María Torres, Olivier Andréoletti, Rosa Bolea
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9ccd201f696e4cacb95cf160a870d459
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spelling	oai:doaj.org-article:9ccd201f696e4cacb95cf160a870d4592021-12-02T19:04:01ZClassical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie10.1038/s41598-021-96818-22045-2322https://doaj.org/article/9ccd201f696e4cacb95cf160a870d4592021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96818-2https://doaj.org/toc/2045-2322Abstract Pigs are susceptible to infection with the classical bovine spongiform encephalopathy (C-BSE) agent following experimental inoculation, and PrPSc accumulation was detected in porcine tissues after the inoculation of certain scrapie and chronic wasting disease isolates. However, a robust transmission barrier has been described in this species and, although they were exposed to C-BSE agent in many European countries, no cases of natural transmissible spongiform encephalopathies (TSE) infections have been reported in pigs. Transmission of atypical scrapie to bovinized mice resulted in the emergence of C-BSE prions. Here, we conducted a study to determine if pigs are susceptible to atypical scrapie. To this end, 12, 8–9-month-old minipigs were intracerebrally inoculated with two atypical scrapie sources. Animals were euthanized between 22- and 72-months post inoculation without clinical signs of TSE. All pigs tested negative for PrPSc accumulation by enzyme immunoassay, immunohistochemistry, western blotting and bioassay in porcine PrP mice. Surprisingly, in vitro protein misfolding cyclic amplification demonstrated the presence of C-BSE prions in different brain areas from seven pigs inoculated with both atypical scrapie isolates. Our results suggest that pigs exposed to atypical scrapie prions could become a reservoir for C-BSE and corroborate that C-BSE prions emerge during interspecies passage of atypical scrapie.Belén MarínAlicia OteroSéverine LuganJuan Carlos EspinosaAlba Marín-MorenoEnric VidalCarlos HedmanAntonio RomeroMartí PumarolaJuan J. BadiolaJuan María TorresOlivier AndréolettiRosa BoleaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Belén Marín Alicia Otero Séverine Lugan Juan Carlos Espinosa Alba Marín-Moreno Enric Vidal Carlos Hedman Antonio Romero Martí Pumarola Juan J. Badiola Juan María Torres Olivier Andréoletti Rosa Bolea Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie
description	Abstract Pigs are susceptible to infection with the classical bovine spongiform encephalopathy (C-BSE) agent following experimental inoculation, and PrPSc accumulation was detected in porcine tissues after the inoculation of certain scrapie and chronic wasting disease isolates. However, a robust transmission barrier has been described in this species and, although they were exposed to C-BSE agent in many European countries, no cases of natural transmissible spongiform encephalopathies (TSE) infections have been reported in pigs. Transmission of atypical scrapie to bovinized mice resulted in the emergence of C-BSE prions. Here, we conducted a study to determine if pigs are susceptible to atypical scrapie. To this end, 12, 8–9-month-old minipigs were intracerebrally inoculated with two atypical scrapie sources. Animals were euthanized between 22- and 72-months post inoculation without clinical signs of TSE. All pigs tested negative for PrPSc accumulation by enzyme immunoassay, immunohistochemistry, western blotting and bioassay in porcine PrP mice. Surprisingly, in vitro protein misfolding cyclic amplification demonstrated the presence of C-BSE prions in different brain areas from seven pigs inoculated with both atypical scrapie isolates. Our results suggest that pigs exposed to atypical scrapie prions could become a reservoir for C-BSE and corroborate that C-BSE prions emerge during interspecies passage of atypical scrapie.
format	article
author	Belén Marín Alicia Otero Séverine Lugan Juan Carlos Espinosa Alba Marín-Moreno Enric Vidal Carlos Hedman Antonio Romero Martí Pumarola Juan J. Badiola Juan María Torres Olivier Andréoletti Rosa Bolea
author_facet	Belén Marín Alicia Otero Séverine Lugan Juan Carlos Espinosa Alba Marín-Moreno Enric Vidal Carlos Hedman Antonio Romero Martí Pumarola Juan J. Badiola Juan María Torres Olivier Andréoletti Rosa Bolea
author_sort	Belén Marín
title	Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie
title_short	Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie
title_full	Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie
title_fullStr	Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie
title_full_unstemmed	Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie
title_sort	classical bse prions emerge from asymptomatic pigs challenged with atypical/nor98 scrapie
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9ccd201f696e4cacb95cf160a870d459
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Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie

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