Activity-dependent modulation of synapse-regulating genes in astrocytes

Astrocytes regulate the formation and function of neuronal synapses via multiple signals; however, what controls regional and temporal expression of these signals during development is unknown. We determined the expression profile of astrocyte synapse-regulating genes in the developing mouse visual...

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Autores principales:	Isabella Farhy-Tselnicker, Matthew M Boisvert, Hanqing Liu, Cari Dowling, Galina A Erikson, Elena Blanco-Suarez, Chen Farhy, Maxim N Shokhirev, Joseph R Ecker, Nicola J Allen
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	astrocytes synaptic terminals visual development gene expression neuronal activity Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9cd09bb480914decb017a9521b470863
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id	oai:doaj.org-article:9cd09bb480914decb017a9521b470863
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spelling	oai:doaj.org-article:9cd09bb480914decb017a9521b4708632021-12-01T11:01:29ZActivity-dependent modulation of synapse-regulating genes in astrocytes10.7554/eLife.705142050-084Xe70514https://doaj.org/article/9cd09bb480914decb017a9521b4708632021-09-01T00:00:00Zhttps://elifesciences.org/articles/70514https://doaj.org/toc/2050-084XAstrocytes regulate the formation and function of neuronal synapses via multiple signals; however, what controls regional and temporal expression of these signals during development is unknown. We determined the expression profile of astrocyte synapse-regulating genes in the developing mouse visual cortex, identifying astrocyte signals that show differential temporal and layer-enriched expression. These patterns are not intrinsic to astrocytes, but regulated by visually evoked neuronal activity, as they are absent in mice lacking glutamate release from thalamocortical terminals. Consequently, synapses remain immature. Expression of synapse-regulating genes and synaptic development is also altered when astrocyte signaling is blunted by diminishing calcium release from astrocyte stores. Single-nucleus RNA sequencing identified groups of astrocytic genes regulated by neuronal and astrocyte activity, and a cassette of genes that show layer-specific enrichment. Thus, the development of cortical circuits requires coordinated signaling between astrocytes and neurons, highlighting astrocytes as a target to manipulate in neurodevelopmental disorders.Isabella Farhy-TselnickerMatthew M BoisvertHanqing LiuCari DowlingGalina A EriksonElena Blanco-SuarezChen FarhyMaxim N ShokhirevJoseph R EckerNicola J AlleneLife Sciences Publications Ltdarticleastrocytessynaptic terminalsvisual developmentgene expressionneuronal activityMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	astrocytes synaptic terminals visual development gene expression neuronal activity Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	astrocytes synaptic terminals visual development gene expression neuronal activity Medicine R Science Q Biology (General) QH301-705.5 Isabella Farhy-Tselnicker Matthew M Boisvert Hanqing Liu Cari Dowling Galina A Erikson Elena Blanco-Suarez Chen Farhy Maxim N Shokhirev Joseph R Ecker Nicola J Allen Activity-dependent modulation of synapse-regulating genes in astrocytes
description	Astrocytes regulate the formation and function of neuronal synapses via multiple signals; however, what controls regional and temporal expression of these signals during development is unknown. We determined the expression profile of astrocyte synapse-regulating genes in the developing mouse visual cortex, identifying astrocyte signals that show differential temporal and layer-enriched expression. These patterns are not intrinsic to astrocytes, but regulated by visually evoked neuronal activity, as they are absent in mice lacking glutamate release from thalamocortical terminals. Consequently, synapses remain immature. Expression of synapse-regulating genes and synaptic development is also altered when astrocyte signaling is blunted by diminishing calcium release from astrocyte stores. Single-nucleus RNA sequencing identified groups of astrocytic genes regulated by neuronal and astrocyte activity, and a cassette of genes that show layer-specific enrichment. Thus, the development of cortical circuits requires coordinated signaling between astrocytes and neurons, highlighting astrocytes as a target to manipulate in neurodevelopmental disorders.
format	article
author	Isabella Farhy-Tselnicker Matthew M Boisvert Hanqing Liu Cari Dowling Galina A Erikson Elena Blanco-Suarez Chen Farhy Maxim N Shokhirev Joseph R Ecker Nicola J Allen
author_facet	Isabella Farhy-Tselnicker Matthew M Boisvert Hanqing Liu Cari Dowling Galina A Erikson Elena Blanco-Suarez Chen Farhy Maxim N Shokhirev Joseph R Ecker Nicola J Allen
author_sort	Isabella Farhy-Tselnicker
title	Activity-dependent modulation of synapse-regulating genes in astrocytes
title_short	Activity-dependent modulation of synapse-regulating genes in astrocytes
title_full	Activity-dependent modulation of synapse-regulating genes in astrocytes
title_fullStr	Activity-dependent modulation of synapse-regulating genes in astrocytes
title_full_unstemmed	Activity-dependent modulation of synapse-regulating genes in astrocytes
title_sort	activity-dependent modulation of synapse-regulating genes in astrocytes
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/9cd09bb480914decb017a9521b470863
work_keys_str_mv	AT isabellafarhytselnicker activitydependentmodulationofsynapseregulatinggenesinastrocytes AT matthewmboisvert activitydependentmodulationofsynapseregulatinggenesinastrocytes AT hanqingliu activitydependentmodulationofsynapseregulatinggenesinastrocytes AT caridowling activitydependentmodulationofsynapseregulatinggenesinastrocytes AT galinaaerikson activitydependentmodulationofsynapseregulatinggenesinastrocytes AT elenablancosuarez activitydependentmodulationofsynapseregulatinggenesinastrocytes AT chenfarhy activitydependentmodulationofsynapseregulatinggenesinastrocytes AT maximnshokhirev activitydependentmodulationofsynapseregulatinggenesinastrocytes AT josephrecker activitydependentmodulationofsynapseregulatinggenesinastrocytes AT nicolajallen activitydependentmodulationofsynapseregulatinggenesinastrocytes
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Activity-dependent modulation of synapse-regulating genes in astrocytes

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