Identitas genetik Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) dari daerah endemik penyakit kuning cabai di Indonesia bagian barat berdasarkan fragmen mitokondria sitokrom oksidase I (mtCOI)
<p><em>Bemisia tabaci </em>(Gennadius) is an important horticulture pest and is know to be a vector of <em>Geminivirus</em>. Pepper yellow leaf curl Indonesia virus (PYLCIV) is caused by <em>Geminivirus</em>, know to be transmitted by <em>B. tabaci<...
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| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | EN ID |
| Publicado: |
The Entomological Society of Indonesia
2017
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/9cd2e4af16784133a570a246682d5726 |
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| Sumario: | <p><em>Bemisia tabaci </em>(Gennadius) is an important horticulture pest and is know to be a vector of <em>Geminivirus</em>. Pepper yellow leaf curl Indonesia virus (PYLCIV) is caused by <em>Geminivirus</em>, know to be transmitted by <em>B. tabaci</em>. It became an important disease in pepper and could cause 100% yield loss. It was limited association informations between vector genetic diversity and its symptom severity. Therefore it is important to investigate <em>B. tabaci </em>genetic variation. Research aim was to observb genetic identity of <em>B. tabaci </em>explored from pepper yellow leaf curl endemic area in west part of Indonesia. Research activities include 1) sample collection of <em>B. tabaci </em>from pepper yellow leaf curl endemic area in west Indonesia; 2) single extraction of <em>B. tabaci </em>total DNA; 3) amplification of mitochondrial Cytochrome Oxidase I gene (<em>mtCOI</em>) fragmen with polymerase chain reaction (PCR); 4) <em>mtCOI</em> sekuencing 5) phylogenetic analysis of <em>mtCOI</em> fragmens from west Indonesian PYLCIV compared with some <em>B. tabaci</em> <em>mtCOI</em> deposited in National Center for Biotechnology Information (NCBI). Based on <em>mtCOI</em> fragment analysis, <em>B. tabaci </em>from PYLCIV endemic area were in one group belong to Asia I clusters. Genetic identity of <em>B. tabaci</em> from endemic area in Western Part of Indonesia were similar with polyatomic cladogram.</p> |
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