Identitas genetik Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) dari daerah endemik penyakit kuning cabai di Indonesia bagian barat berdasarkan fragmen mitokondria sitokrom oksidase I (mtCOI)

Bemisia tabaci (Gennadius) is an important horticulture pest and is know to be a vector of Geminivirus. Pepper yellow leaf curl Indonesia virus (PYLCIV) is caused by Geminivirus, know to be transmitted by B. tabaci<...

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Autores principales:	Sat Rahayuwati, Sri Hendrastuti Hidayat, Purnama Hidayat
Formato:	article
Lenguaje:	EN ID
Publicado:	The Entomological Society of Indonesia 2017
Materias:	Asia I cluster, Bemisia tabaci, Geminivirus vectors, mtCOI, non B biotype Zoology QL1-991
Acceso en línea:	https://doaj.org/article/9cd2e4af16784133a570a246682d5726
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spelling	oai:doaj.org-article:9cd2e4af16784133a570a246682d57262021-12-02T00:22:28ZIdentitas genetik Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) dari daerah endemik penyakit kuning cabai di Indonesia bagian barat berdasarkan fragmen mitokondria sitokrom oksidase I (mtCOI)1829-77222089-025710.5994/jei.13.3.156https://doaj.org/article/9cd2e4af16784133a570a246682d57262017-06-01T00:00:00Zhttp://jurnal.pei-pusat.org/index.php/jei/article/view/175https://doaj.org/toc/1829-7722https://doaj.org/toc/2089-0257<p><em>Bemisia tabaci </em>(Gennadius) is an important horticulture pest and is know to be a vector of <em>Geminivirus</em>. Pepper yellow leaf curl Indonesia virus (PYLCIV) is caused by <em>Geminivirus</em>, know to be transmitted by <em>B. tabaci</em>. It became an important disease in pepper and could cause 100% yield loss. It was limited association informations between vector genetic diversity and its symptom severity. Therefore it is important to investigate <em>B. tabaci </em>genetic variation. Research aim was to observb genetic identity of <em>B. tabaci </em>explored from pepper yellow leaf curl endemic area in west part of Indonesia. Research activities include 1) sample collection of <em>B. tabaci </em>from pepper yellow leaf curl endemic area in west Indonesia; 2) single extraction of <em>B. tabaci </em>total DNA; 3) amplification of mitochondrial Cytochrome Oxidase I gene (<em>mtCOI</em>) fragmen with polymerase chain reaction (PCR); 4) <em>mtCOI</em> sekuencing 5) phylogenetic analysis of <em>mtCOI</em> fragmens from west Indonesian PYLCIV compared with some <em>B. tabaci</em> <em>mtCOI</em> deposited in National Center for Biotechnology Information (NCBI). Based on <em>mtCOI</em> fragment analysis, <em>B. tabaci </em>from PYLCIV endemic area were in one group belong to Asia I clusters. Genetic identity of <em>B. tabaci</em> from endemic area in Western Part of Indonesia were similar with polyatomic cladogram.</p>Sat RahayuwatiSri Hendrastuti HidayatPurnama HidayatThe Entomological Society of IndonesiaarticleAsia I cluster, Bemisia tabaci, Geminivirus vectors, mtCOI, non B biotypeZoologyQL1-991ENIDJurnal Entomologi Indonesia, Vol 13, Iss 3 (2017)
institution	DOAJ
collection	DOAJ
language	EN ID
topic	Asia I cluster, Bemisia tabaci, Geminivirus vectors, mtCOI, non B biotype Zoology QL1-991
spellingShingle	Asia I cluster, Bemisia tabaci, Geminivirus vectors, mtCOI, non B biotype Zoology QL1-991 Sat Rahayuwati Sri Hendrastuti Hidayat Purnama Hidayat Identitas genetik Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) dari daerah endemik penyakit kuning cabai di Indonesia bagian barat berdasarkan fragmen mitokondria sitokrom oksidase I (mtCOI)
description	<p><em>Bemisia tabaci </em>(Gennadius) is an important horticulture pest and is know to be a vector of <em>Geminivirus</em>. Pepper yellow leaf curl Indonesia virus (PYLCIV) is caused by <em>Geminivirus</em>, know to be transmitted by <em>B. tabaci</em>. It became an important disease in pepper and could cause 100% yield loss. It was limited association informations between vector genetic diversity and its symptom severity. Therefore it is important to investigate <em>B. tabaci </em>genetic variation. Research aim was to observb genetic identity of <em>B. tabaci </em>explored from pepper yellow leaf curl endemic area in west part of Indonesia. Research activities include 1) sample collection of <em>B. tabaci </em>from pepper yellow leaf curl endemic area in west Indonesia; 2) single extraction of <em>B. tabaci </em>total DNA; 3) amplification of mitochondrial Cytochrome Oxidase I gene (<em>mtCOI</em>) fragmen with polymerase chain reaction (PCR); 4) <em>mtCOI</em> sekuencing 5) phylogenetic analysis of <em>mtCOI</em> fragmens from west Indonesian PYLCIV compared with some <em>B. tabaci</em> <em>mtCOI</em> deposited in National Center for Biotechnology Information (NCBI). Based on <em>mtCOI</em> fragment analysis, <em>B. tabaci </em>from PYLCIV endemic area were in one group belong to Asia I clusters. Genetic identity of <em>B. tabaci</em> from endemic area in Western Part of Indonesia were similar with polyatomic cladogram.</p>
format	article
author	Sat Rahayuwati Sri Hendrastuti Hidayat Purnama Hidayat
author_facet	Sat Rahayuwati Sri Hendrastuti Hidayat Purnama Hidayat
author_sort	Sat Rahayuwati
title	Identitas genetik Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) dari daerah endemik penyakit kuning cabai di Indonesia bagian barat berdasarkan fragmen mitokondria sitokrom oksidase I (mtCOI)
title_short	Identitas genetik Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) dari daerah endemik penyakit kuning cabai di Indonesia bagian barat berdasarkan fragmen mitokondria sitokrom oksidase I (mtCOI)
title_full	Identitas genetik Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) dari daerah endemik penyakit kuning cabai di Indonesia bagian barat berdasarkan fragmen mitokondria sitokrom oksidase I (mtCOI)
title_fullStr	Identitas genetik Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) dari daerah endemik penyakit kuning cabai di Indonesia bagian barat berdasarkan fragmen mitokondria sitokrom oksidase I (mtCOI)
title_full_unstemmed	Identitas genetik Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) dari daerah endemik penyakit kuning cabai di Indonesia bagian barat berdasarkan fragmen mitokondria sitokrom oksidase I (mtCOI)
title_sort	identitas genetik bemisia tabaci (gennadius) (hemiptera: aleyrodidae) dari daerah endemik penyakit kuning cabai di indonesia bagian barat berdasarkan fragmen mitokondria sitokrom oksidase i (mtcoi)
publisher	The Entomological Society of Indonesia
publishDate	2017
url	https://doaj.org/article/9cd2e4af16784133a570a246682d5726
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Identitas genetik Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) dari daerah endemik penyakit kuning cabai di Indonesia bagian barat berdasarkan fragmen mitokondria sitokrom oksidase I (mtCOI)

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