HIG1 domain family member 1A disrupts proliferation, migration and invasion of colon adenocarcinoma cells

HIG1 domain family member 1A (Higd-1a) interacts with dynamin-like 120 kDa protein to maintain the morphological and functional integrity of the mitochondria and thus plays an important role in the progression of malignant tumors. Higd-1a promotes the proliferation of pancreatic cancer cells and the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhenyu Xu, Junjie Sun, Yang Chen, Ting Zhang, Yan Qin, Dong Hua
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	hig1 domain family member 1a colorectal cancer proliferation Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/9cd6e79feedd483f957b4573ab889015
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9cd6e79feedd483f957b4573ab889015
record_format	dspace
spelling	oai:doaj.org-article:9cd6e79feedd483f957b4573ab8890152021-11-26T11:19:49ZHIG1 domain family member 1A disrupts proliferation, migration and invasion of colon adenocarcinoma cells2165-59792165-598710.1080/21655979.2021.1999368https://doaj.org/article/9cd6e79feedd483f957b4573ab8890152021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1999368https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987HIG1 domain family member 1A (Higd-1a) interacts with dynamin-like 120 kDa protein to maintain the morphological and functional integrity of the mitochondria and thus plays an important role in the progression of malignant tumors. Higd-1a promotes the proliferation of pancreatic cancer cells and the growth of pancreatic cancer; however, no similar observations have been reported for colorectal cancer (CRC). This study, therefore, aimed to verify the role of Higd-1a in CRC. We downloaded data from the Genotype-Tissue Expression (GTEX) and The Cancer Genome Atlas (TCGA) databases and identified an association between Higd-1a levels in colon adenocarcinoma (COAD) tissues and poor survival using Kaplan–Meier curves. Subsequently, we overexpressed Higd-1a in the human COAD cell line HCT-8, knocked down Higd-1a expression in SW480 cells, and evaluated the effects via quantitative PCR (qPCR) and western blotting. MTT assays, colony formation assay, cell cycle analysis, annexin V-FITC/PI, wound-healing analysis, and transwell assay were used to test cell proliferation, formation of cell colonies, cell cycle progression, migration, invasiveness, and apoptosis. Higd-1a has low transcription levels in COAD tissue and suggests a poor prognosis. Higd-1a overexpression in HCT-8 cells weakened cell proliferation, formation of cell colonies, cell cycle progression, migration ability, and invasiveness, and increased apoptosis. Moreover, the decrease of Higd-1a in SW480 cells induced cell proliferation, formation of cell colonies, cell cycle progression, migration, and invasion, and inhibited apoptosis. Higd-1a is underexpressed in COAD cells and its overexpression impaired the proliferation, migration, and invasiveness of COAD cells.Zhenyu XuJunjie SunYang ChenTing ZhangYan QinDong HuaTaylor & Francis Grouparticlehig1 domain family member 1acolorectal cancerproliferationBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hig1 domain family member 1a colorectal cancer proliferation Biotechnology TP248.13-248.65
spellingShingle	hig1 domain family member 1a colorectal cancer proliferation Biotechnology TP248.13-248.65 Zhenyu Xu Junjie Sun Yang Chen Ting Zhang Yan Qin Dong Hua HIG1 domain family member 1A disrupts proliferation, migration and invasion of colon adenocarcinoma cells
description	HIG1 domain family member 1A (Higd-1a) interacts with dynamin-like 120 kDa protein to maintain the morphological and functional integrity of the mitochondria and thus plays an important role in the progression of malignant tumors. Higd-1a promotes the proliferation of pancreatic cancer cells and the growth of pancreatic cancer; however, no similar observations have been reported for colorectal cancer (CRC). This study, therefore, aimed to verify the role of Higd-1a in CRC. We downloaded data from the Genotype-Tissue Expression (GTEX) and The Cancer Genome Atlas (TCGA) databases and identified an association between Higd-1a levels in colon adenocarcinoma (COAD) tissues and poor survival using Kaplan–Meier curves. Subsequently, we overexpressed Higd-1a in the human COAD cell line HCT-8, knocked down Higd-1a expression in SW480 cells, and evaluated the effects via quantitative PCR (qPCR) and western blotting. MTT assays, colony formation assay, cell cycle analysis, annexin V-FITC/PI, wound-healing analysis, and transwell assay were used to test cell proliferation, formation of cell colonies, cell cycle progression, migration, invasiveness, and apoptosis. Higd-1a has low transcription levels in COAD tissue and suggests a poor prognosis. Higd-1a overexpression in HCT-8 cells weakened cell proliferation, formation of cell colonies, cell cycle progression, migration ability, and invasiveness, and increased apoptosis. Moreover, the decrease of Higd-1a in SW480 cells induced cell proliferation, formation of cell colonies, cell cycle progression, migration, and invasion, and inhibited apoptosis. Higd-1a is underexpressed in COAD cells and its overexpression impaired the proliferation, migration, and invasiveness of COAD cells.
format	article
author	Zhenyu Xu Junjie Sun Yang Chen Ting Zhang Yan Qin Dong Hua
author_facet	Zhenyu Xu Junjie Sun Yang Chen Ting Zhang Yan Qin Dong Hua
author_sort	Zhenyu Xu
title	HIG1 domain family member 1A disrupts proliferation, migration and invasion of colon adenocarcinoma cells
title_short	HIG1 domain family member 1A disrupts proliferation, migration and invasion of colon adenocarcinoma cells
title_full	HIG1 domain family member 1A disrupts proliferation, migration and invasion of colon adenocarcinoma cells
title_fullStr	HIG1 domain family member 1A disrupts proliferation, migration and invasion of colon adenocarcinoma cells
title_full_unstemmed	HIG1 domain family member 1A disrupts proliferation, migration and invasion of colon adenocarcinoma cells
title_sort	hig1 domain family member 1a disrupts proliferation, migration and invasion of colon adenocarcinoma cells
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/9cd6e79feedd483f957b4573ab889015
work_keys_str_mv	AT zhenyuxu hig1domainfamilymember1adisruptsproliferationmigrationandinvasionofcolonadenocarcinomacells AT junjiesun hig1domainfamilymember1adisruptsproliferationmigrationandinvasionofcolonadenocarcinomacells AT yangchen hig1domainfamilymember1adisruptsproliferationmigrationandinvasionofcolonadenocarcinomacells AT tingzhang hig1domainfamilymember1adisruptsproliferationmigrationandinvasionofcolonadenocarcinomacells AT yanqin hig1domainfamilymember1adisruptsproliferationmigrationandinvasionofcolonadenocarcinomacells AT donghua hig1domainfamilymember1adisruptsproliferationmigrationandinvasionofcolonadenocarcinomacells
_version_	1718409489183408128

HIG1 domain family member 1A disrupts proliferation, migration and invasion of colon adenocarcinoma cells

Ejemplares similares