Comparative Metabolomics Analysis Reveals Sterols and Sphingolipids Play a Role in Cotton Fiber Cell Initiation

Cotton fiber is a seed trichome that protrudes from the outer epidermis of cotton ovule on the day of anthesis (0 day past anthesis, 0 DPA). The initial number and timing of fiber cells are closely related to fiber yield and quality. However, the mechanism underlying fiber initiation is still unclea...

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Autores principales:	Qiaoling Wang, Qian Meng, Fan Xu, Qian Chen, Caixia Ma, Li Huang, Guiming Li, Ming Luo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	sphingolipids sphingolipid metabolism sterol cotton fiber cell initiation Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/9cdfec3e7808468e98ca66fe06ae2a03
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spelling	oai:doaj.org-article:9cdfec3e7808468e98ca66fe06ae2a032021-11-11T16:54:17ZComparative Metabolomics Analysis Reveals Sterols and Sphingolipids Play a Role in Cotton Fiber Cell Initiation10.3390/ijms2221114381422-00671661-6596https://doaj.org/article/9cdfec3e7808468e98ca66fe06ae2a032021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11438https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Cotton fiber is a seed trichome that protrudes from the outer epidermis of cotton ovule on the day of anthesis (0 day past anthesis, 0 DPA). The initial number and timing of fiber cells are closely related to fiber yield and quality. However, the mechanism underlying fiber initiation is still unclear. Here, we detected and compared the contents and compositions of sphingolipids and sterols in 0 DPA ovules of <i>Xuzhou142 lintless-fuzzless mutants</i> (<i>Xufl</i>) and <i>Xinxiangxiaoji lintless-fuzzless mutants</i> (<i>Xinfl</i>) and upland cotton wild-type Xuzhou142 (XuFL). Nine classes of sphingolipids and sixty-six sphingolipid molecular species were detected in wild-type and mutants. Compared with the wild type, the contents of Sphingosine-1-phosphate (S1P), Sphingosine (Sph), Glucosylceramide (GluCer), and Glycosyl-inositol-phospho-ceramides (GIPC) were decreased in the mutants, while the contents of Ceramide (Cer) were increased. Detail, the contents of two Cer molecular species, d18:1/22:0 and d18:1/24:0, and two Phyto-Cer molecular species, t18:0/22:0 and t18:0/h22:1 were significantly increased, while the contents of all GluCer and GIPC molecular species were decreased. Consistent with this result, the expression levels of seven genes involved in GluCer and GIPC synthesis were decreased in the mutants. Furthermore, exogenous application of a specific inhibitor of GluCer synthase, PDMP (1-phenyl-2-decanoylamino-3-morpholino-1-propanol), in ovule culture system, significantly inhibited the initiation of cotton fiber cells. In addition, five sterols and four sterol esters were detected in wild-type and mutant ovules. Compared with the wild type, the contents of total sterol were not significantly changed. While the contents of stigmasterol and campesterol were significantly increased, the contents of cholesterol were significantly decreased, and the contents of total sterol esters were significantly increased. In particular, the contents of campesterol esters and stigmasterol esters increased significantly in the two mutants. Consistently, the expression levels of some sterol synthase genes and sterol ester synthase genes were also changed in the two mutants. These results suggested that sphingolipids and sterols might have some roles in the initiation of fiber cells. Our results provided a novel insight into the regulatory mechanism of fiber cell initiation.Qiaoling WangQian MengFan XuQian ChenCaixia MaLi HuangGuiming LiMing LuoMDPI AGarticlesphingolipidssphingolipid metabolismsterolcottonfiber cellinitiationBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11438, p 11438 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	sphingolipids sphingolipid metabolism sterol cotton fiber cell initiation Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	sphingolipids sphingolipid metabolism sterol cotton fiber cell initiation Biology (General) QH301-705.5 Chemistry QD1-999 Qiaoling Wang Qian Meng Fan Xu Qian Chen Caixia Ma Li Huang Guiming Li Ming Luo Comparative Metabolomics Analysis Reveals Sterols and Sphingolipids Play a Role in Cotton Fiber Cell Initiation
description	Cotton fiber is a seed trichome that protrudes from the outer epidermis of cotton ovule on the day of anthesis (0 day past anthesis, 0 DPA). The initial number and timing of fiber cells are closely related to fiber yield and quality. However, the mechanism underlying fiber initiation is still unclear. Here, we detected and compared the contents and compositions of sphingolipids and sterols in 0 DPA ovules of <i>Xuzhou142 lintless-fuzzless mutants</i> (<i>Xufl</i>) and <i>Xinxiangxiaoji lintless-fuzzless mutants</i> (<i>Xinfl</i>) and upland cotton wild-type Xuzhou142 (XuFL). Nine classes of sphingolipids and sixty-six sphingolipid molecular species were detected in wild-type and mutants. Compared with the wild type, the contents of Sphingosine-1-phosphate (S1P), Sphingosine (Sph), Glucosylceramide (GluCer), and Glycosyl-inositol-phospho-ceramides (GIPC) were decreased in the mutants, while the contents of Ceramide (Cer) were increased. Detail, the contents of two Cer molecular species, d18:1/22:0 and d18:1/24:0, and two Phyto-Cer molecular species, t18:0/22:0 and t18:0/h22:1 were significantly increased, while the contents of all GluCer and GIPC molecular species were decreased. Consistent with this result, the expression levels of seven genes involved in GluCer and GIPC synthesis were decreased in the mutants. Furthermore, exogenous application of a specific inhibitor of GluCer synthase, PDMP (1-phenyl-2-decanoylamino-3-morpholino-1-propanol), in ovule culture system, significantly inhibited the initiation of cotton fiber cells. In addition, five sterols and four sterol esters were detected in wild-type and mutant ovules. Compared with the wild type, the contents of total sterol were not significantly changed. While the contents of stigmasterol and campesterol were significantly increased, the contents of cholesterol were significantly decreased, and the contents of total sterol esters were significantly increased. In particular, the contents of campesterol esters and stigmasterol esters increased significantly in the two mutants. Consistently, the expression levels of some sterol synthase genes and sterol ester synthase genes were also changed in the two mutants. These results suggested that sphingolipids and sterols might have some roles in the initiation of fiber cells. Our results provided a novel insight into the regulatory mechanism of fiber cell initiation.
format	article
author	Qiaoling Wang Qian Meng Fan Xu Qian Chen Caixia Ma Li Huang Guiming Li Ming Luo
author_facet	Qiaoling Wang Qian Meng Fan Xu Qian Chen Caixia Ma Li Huang Guiming Li Ming Luo
author_sort	Qiaoling Wang
title	Comparative Metabolomics Analysis Reveals Sterols and Sphingolipids Play a Role in Cotton Fiber Cell Initiation
title_short	Comparative Metabolomics Analysis Reveals Sterols and Sphingolipids Play a Role in Cotton Fiber Cell Initiation
title_full	Comparative Metabolomics Analysis Reveals Sterols and Sphingolipids Play a Role in Cotton Fiber Cell Initiation
title_fullStr	Comparative Metabolomics Analysis Reveals Sterols and Sphingolipids Play a Role in Cotton Fiber Cell Initiation
title_full_unstemmed	Comparative Metabolomics Analysis Reveals Sterols and Sphingolipids Play a Role in Cotton Fiber Cell Initiation
title_sort	comparative metabolomics analysis reveals sterols and sphingolipids play a role in cotton fiber cell initiation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9cdfec3e7808468e98ca66fe06ae2a03
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Comparative Metabolomics Analysis Reveals Sterols and Sphingolipids Play a Role in Cotton Fiber Cell Initiation

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