Juvenile Hormone Epoxide Hydrolase: a Promising Target for Hemipteran Pest Management

Abstract Juvenile hormone epoxide hydrolase (JHEH) has attracted great interest because of its critical role in the regulation of juvenile hormone (JH) in insects. In this study, one JHEH gene from Apolygus lucorum (AlucJHEH) was characterized in terms of deduced amino acid sequence, phylogeny, homo...

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Autores principales:	Abudourusuli Tusun, Ming Li, Xiangzhi Liang, Ting Yang, Bin Yang, Guirong Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9ce59b914e914e28b5308606bb4d0677
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spelling	oai:doaj.org-article:9ce59b914e914e28b5308606bb4d06772021-12-02T15:06:19ZJuvenile Hormone Epoxide Hydrolase: a Promising Target for Hemipteran Pest Management10.1038/s41598-017-00907-02045-2322https://doaj.org/article/9ce59b914e914e28b5308606bb4d06772017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00907-0https://doaj.org/toc/2045-2322Abstract Juvenile hormone epoxide hydrolase (JHEH) has attracted great interest because of its critical role in the regulation of juvenile hormone (JH) in insects. In this study, one JHEH gene from Apolygus lucorum (AlucJHEH) was characterized in terms of deduced amino acid sequence, phylogeny, homology modeling and docking simulation. The results reveals a conserved catalytic mechanism of AlucJHEH toward JH. Our study also demonstrates that the mRNA of AlucJHEH gene was detectable in head, thorax and abdomen from all life stages. To functionally characterize the AlucJHEH gene, three fragments of double-stranded RNAs (dsRNAs) were designed to target different regions of the sequence. Injection of 3rd nymphs with dsRNA fragments successfully knocked down the target gene expression, and a significantly decreased survival rate was observed, together with a molting block, These findings confirm the important regulatory roles of AlucJHEH in A. lucorum and indicate this gene as a promising target for future hemipterans pest control.Abudourusuli TusunMing LiXiangzhi LiangTing YangBin YangGuirong WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Abudourusuli Tusun Ming Li Xiangzhi Liang Ting Yang Bin Yang Guirong Wang Juvenile Hormone Epoxide Hydrolase: a Promising Target for Hemipteran Pest Management
description	Abstract Juvenile hormone epoxide hydrolase (JHEH) has attracted great interest because of its critical role in the regulation of juvenile hormone (JH) in insects. In this study, one JHEH gene from Apolygus lucorum (AlucJHEH) was characterized in terms of deduced amino acid sequence, phylogeny, homology modeling and docking simulation. The results reveals a conserved catalytic mechanism of AlucJHEH toward JH. Our study also demonstrates that the mRNA of AlucJHEH gene was detectable in head, thorax and abdomen from all life stages. To functionally characterize the AlucJHEH gene, three fragments of double-stranded RNAs (dsRNAs) were designed to target different regions of the sequence. Injection of 3rd nymphs with dsRNA fragments successfully knocked down the target gene expression, and a significantly decreased survival rate was observed, together with a molting block, These findings confirm the important regulatory roles of AlucJHEH in A. lucorum and indicate this gene as a promising target for future hemipterans pest control.
format	article
author	Abudourusuli Tusun Ming Li Xiangzhi Liang Ting Yang Bin Yang Guirong Wang
author_facet	Abudourusuli Tusun Ming Li Xiangzhi Liang Ting Yang Bin Yang Guirong Wang
author_sort	Abudourusuli Tusun
title	Juvenile Hormone Epoxide Hydrolase: a Promising Target for Hemipteran Pest Management
title_short	Juvenile Hormone Epoxide Hydrolase: a Promising Target for Hemipteran Pest Management
title_full	Juvenile Hormone Epoxide Hydrolase: a Promising Target for Hemipteran Pest Management
title_fullStr	Juvenile Hormone Epoxide Hydrolase: a Promising Target for Hemipteran Pest Management
title_full_unstemmed	Juvenile Hormone Epoxide Hydrolase: a Promising Target for Hemipteran Pest Management
title_sort	juvenile hormone epoxide hydrolase: a promising target for hemipteran pest management
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9ce59b914e914e28b5308606bb4d0677
work_keys_str_mv	AT abudourusulitusun juvenilehormoneepoxidehydrolaseapromisingtargetforhemipteranpestmanagement AT mingli juvenilehormoneepoxidehydrolaseapromisingtargetforhemipteranpestmanagement AT xiangzhiliang juvenilehormoneepoxidehydrolaseapromisingtargetforhemipteranpestmanagement AT tingyang juvenilehormoneepoxidehydrolaseapromisingtargetforhemipteranpestmanagement AT binyang juvenilehormoneepoxidehydrolaseapromisingtargetforhemipteranpestmanagement AT guirongwang juvenilehormoneepoxidehydrolaseapromisingtargetforhemipteranpestmanagement
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Juvenile Hormone Epoxide Hydrolase: a Promising Target for Hemipteran Pest Management

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