L-Menthol-Loadable Electrospun Fibers of PMVEMA Anhydride for Topical Administration

Poly(methyl vinyl ether-<i>alt</i>-maleic anhydride) (PMVEMA) of 119 and 139 molecular weights (P119 and P139, respectively) were electrospun to evaluate the resulting fibers as a topical delivery vehicle for (L-)menthol. Thus, electrospinning parameters were optimized for the production...

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Autores principales:	Amalia Mira, Marta Rubio-Camacho, David Alarcón, Enrique Rodríguez-Cañas, Asia Fernández-Carvajal, Alberto Falco, Ricardo Mallavia
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	PMVEMA electrospinning nanofibers microfibers menthol viability Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/9cf2610eea5542eba130c70be947cd3b
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spelling	oai:doaj.org-article:9cf2610eea5542eba130c70be947cd3b2021-11-25T18:41:07ZL-Menthol-Loadable Electrospun Fibers of PMVEMA Anhydride for Topical Administration10.3390/pharmaceutics131118451999-4923https://doaj.org/article/9cf2610eea5542eba130c70be947cd3b2021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1845https://doaj.org/toc/1999-4923Poly(methyl vinyl ether-<i>alt</i>-maleic anhydride) (PMVEMA) of 119 and 139 molecular weights (P119 and P139, respectively) were electrospun to evaluate the resulting fibers as a topical delivery vehicle for (L-)menthol. Thus, electrospinning parameters were optimized for the production of uniform bead-free fibers from 12% <i>w</i>/<i>w</i> PMVEMA (±2.3% <i>w</i>/<i>w</i> menthol) solutions, and their morphology and size were characterized by field emission scanning electron microscopy (FESEM). The fibers of P119 (F<sub>119</sub>s) and P139 (F<sub>139</sub>s) showed average diameter sizes of approximately 534 and 664 nm, respectively, when unloaded, and 837 and 1369 nm when loaded with menthol. The morphology of all types of fibers was cylindrical except for F<sub>139</sub>s, which mostly displayed a double-ribbon-like shape. Gas chromatography-mass spectrometry (GC-MS) analysis determined that not only was the menthol encapsulation efficiency higher in F<sub>139</sub>s (92% versus 68% in F<sub>119</sub>s) but also that its stability over time was higher, given that in contrast with F<sub>119</sub>s, no significant losses in encapsulated menthol were detected in the F<sub>139</sub>s after 10 days post-production. Finally, in vitro biological assays showed no significant induction of cytotoxicity for any of the experimental fibers or in the full functionality of the encapsulated menthol, as it achieved equivalent free-menthol levels of activation of its specific receptor, the (human) transient receptor potential cation channel subfamily M (melastatin) member 8 (TRPM8).Amalia MiraMarta Rubio-CamachoDavid AlarcónEnrique Rodríguez-CañasAsia Fernández-CarvajalAlberto FalcoRicardo MallaviaMDPI AGarticlePMVEMAelectrospinningnanofibersmicrofibersmentholviabilityPharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1845, p 1845 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	PMVEMA electrospinning nanofibers microfibers menthol viability Pharmacy and materia medica RS1-441
spellingShingle	PMVEMA electrospinning nanofibers microfibers menthol viability Pharmacy and materia medica RS1-441 Amalia Mira Marta Rubio-Camacho David Alarcón Enrique Rodríguez-Cañas Asia Fernández-Carvajal Alberto Falco Ricardo Mallavia L-Menthol-Loadable Electrospun Fibers of PMVEMA Anhydride for Topical Administration
description	Poly(methyl vinyl ether-<i>alt</i>-maleic anhydride) (PMVEMA) of 119 and 139 molecular weights (P119 and P139, respectively) were electrospun to evaluate the resulting fibers as a topical delivery vehicle for (L-)menthol. Thus, electrospinning parameters were optimized for the production of uniform bead-free fibers from 12% <i>w</i>/<i>w</i> PMVEMA (±2.3% <i>w</i>/<i>w</i> menthol) solutions, and their morphology and size were characterized by field emission scanning electron microscopy (FESEM). The fibers of P119 (F<sub>119</sub>s) and P139 (F<sub>139</sub>s) showed average diameter sizes of approximately 534 and 664 nm, respectively, when unloaded, and 837 and 1369 nm when loaded with menthol. The morphology of all types of fibers was cylindrical except for F<sub>139</sub>s, which mostly displayed a double-ribbon-like shape. Gas chromatography-mass spectrometry (GC-MS) analysis determined that not only was the menthol encapsulation efficiency higher in F<sub>139</sub>s (92% versus 68% in F<sub>119</sub>s) but also that its stability over time was higher, given that in contrast with F<sub>119</sub>s, no significant losses in encapsulated menthol were detected in the F<sub>139</sub>s after 10 days post-production. Finally, in vitro biological assays showed no significant induction of cytotoxicity for any of the experimental fibers or in the full functionality of the encapsulated menthol, as it achieved equivalent free-menthol levels of activation of its specific receptor, the (human) transient receptor potential cation channel subfamily M (melastatin) member 8 (TRPM8).
format	article
author	Amalia Mira Marta Rubio-Camacho David Alarcón Enrique Rodríguez-Cañas Asia Fernández-Carvajal Alberto Falco Ricardo Mallavia
author_facet	Amalia Mira Marta Rubio-Camacho David Alarcón Enrique Rodríguez-Cañas Asia Fernández-Carvajal Alberto Falco Ricardo Mallavia
author_sort	Amalia Mira
title	L-Menthol-Loadable Electrospun Fibers of PMVEMA Anhydride for Topical Administration
title_short	L-Menthol-Loadable Electrospun Fibers of PMVEMA Anhydride for Topical Administration
title_full	L-Menthol-Loadable Electrospun Fibers of PMVEMA Anhydride for Topical Administration
title_fullStr	L-Menthol-Loadable Electrospun Fibers of PMVEMA Anhydride for Topical Administration
title_full_unstemmed	L-Menthol-Loadable Electrospun Fibers of PMVEMA Anhydride for Topical Administration
title_sort	l-menthol-loadable electrospun fibers of pmvema anhydride for topical administration
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9cf2610eea5542eba130c70be947cd3b
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L-Menthol-Loadable Electrospun Fibers of PMVEMA Anhydride for Topical Administration

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