Pyrocystis noctiluca represents an excellent bioassay for shear forces induced in ground-based microgravity simulators (clinostat and random positioning machine)
Microgravity simulators: Bio-reporters illuminate false signals Earth-based laboratories can now assess the accuracy of tools used to simulate living organism growth and behaviour in space with bioluminescent assays. Researchers often use rotating machines to minimize gravity effects during the desi...
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| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | EN |
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Nature Portfolio
2017
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/9cf3c01b44894b15823b9e1aaccfc06e |
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| Sumario: | Microgravity simulators: Bio-reporters illuminate false signals Earth-based laboratories can now assess the accuracy of tools used to simulate living organism growth and behaviour in space with bioluminescent assays. Researchers often use rotating machines to minimize gravity effects during the design of extra-terrestrial experiments with plants, cells, and small animals. Jens Hauslage from the DLR German Aerospace Center and colleagues report that device-specific shear forces produced during mechanical movements may cause misinterpretations of initial test data. They developed a biosensor based on marine plankton, known as dinoflagellates, which have cell membranes that naturally emit light when touched by predators. Calibrating this bioluminescence against mechanical stress helped determine the top-like, 2D rotations of ‘‘clinostat’’ devices provided microgravity-like conditions. However, the unexpected 3D movements of Random Positioning Machines generated enough shear force to impact studies of cell signaling pathways or metabolic reactions. |
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