Pyrocystis noctiluca represents an excellent bioassay for shear forces induced in ground-based microgravity simulators (clinostat and random positioning machine)

Microgravity simulators: Bio-reporters illuminate false signals Earth-based laboratories can now assess the accuracy of tools used to simulate living organism growth and behaviour in space with bioluminescent assays. Researchers often use rotating machines to minimize gravity effects during the desi...

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Autores principales:	Jens Hauslage, Volkan Cevik, Ruth Hemmersbach
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Biotechnology TP248.13-248.65 Physiology QP1-981
Acceso en línea:	https://doaj.org/article/9cf3c01b44894b15823b9e1aaccfc06e
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spelling	oai:doaj.org-article:9cf3c01b44894b15823b9e1aaccfc06e2021-12-02T12:30:49ZPyrocystis noctiluca represents an excellent bioassay for shear forces induced in ground-based microgravity simulators (clinostat and random positioning machine)10.1038/s41526-017-0016-x2373-8065https://doaj.org/article/9cf3c01b44894b15823b9e1aaccfc06e2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41526-017-0016-xhttps://doaj.org/toc/2373-8065Microgravity simulators: Bio-reporters illuminate false signals Earth-based laboratories can now assess the accuracy of tools used to simulate living organism growth and behaviour in space with bioluminescent assays. Researchers often use rotating machines to minimize gravity effects during the design of extra-terrestrial experiments with plants, cells, and small animals. Jens Hauslage from the DLR German Aerospace Center and colleagues report that device-specific shear forces produced during mechanical movements may cause misinterpretations of initial test data. They developed a biosensor based on marine plankton, known as dinoflagellates, which have cell membranes that naturally emit light when touched by predators. Calibrating this bioluminescence against mechanical stress helped determine the top-like, 2D rotations of ‘‘clinostat’’ devices provided microgravity-like conditions. However, the unexpected 3D movements of Random Positioning Machines generated enough shear force to impact studies of cell signaling pathways or metabolic reactions.Jens HauslageVolkan CevikRuth HemmersbachNature PortfolioarticleBiotechnologyTP248.13-248.65PhysiologyQP1-981ENnpj Microgravity, Vol 3, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65 Physiology QP1-981
spellingShingle	Biotechnology TP248.13-248.65 Physiology QP1-981 Jens Hauslage Volkan Cevik Ruth Hemmersbach Pyrocystis noctiluca represents an excellent bioassay for shear forces induced in ground-based microgravity simulators (clinostat and random positioning machine)
description	Microgravity simulators: Bio-reporters illuminate false signals Earth-based laboratories can now assess the accuracy of tools used to simulate living organism growth and behaviour in space with bioluminescent assays. Researchers often use rotating machines to minimize gravity effects during the design of extra-terrestrial experiments with plants, cells, and small animals. Jens Hauslage from the DLR German Aerospace Center and colleagues report that device-specific shear forces produced during mechanical movements may cause misinterpretations of initial test data. They developed a biosensor based on marine plankton, known as dinoflagellates, which have cell membranes that naturally emit light when touched by predators. Calibrating this bioluminescence against mechanical stress helped determine the top-like, 2D rotations of ‘‘clinostat’’ devices provided microgravity-like conditions. However, the unexpected 3D movements of Random Positioning Machines generated enough shear force to impact studies of cell signaling pathways or metabolic reactions.
format	article
author	Jens Hauslage Volkan Cevik Ruth Hemmersbach
author_facet	Jens Hauslage Volkan Cevik Ruth Hemmersbach
author_sort	Jens Hauslage
title	Pyrocystis noctiluca represents an excellent bioassay for shear forces induced in ground-based microgravity simulators (clinostat and random positioning machine)
title_short	Pyrocystis noctiluca represents an excellent bioassay for shear forces induced in ground-based microgravity simulators (clinostat and random positioning machine)
title_full	Pyrocystis noctiluca represents an excellent bioassay for shear forces induced in ground-based microgravity simulators (clinostat and random positioning machine)
title_fullStr	Pyrocystis noctiluca represents an excellent bioassay for shear forces induced in ground-based microgravity simulators (clinostat and random positioning machine)
title_full_unstemmed	Pyrocystis noctiluca represents an excellent bioassay for shear forces induced in ground-based microgravity simulators (clinostat and random positioning machine)
title_sort	pyrocystis noctiluca represents an excellent bioassay for shear forces induced in ground-based microgravity simulators (clinostat and random positioning machine)
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9cf3c01b44894b15823b9e1aaccfc06e
work_keys_str_mv	AT jenshauslage pyrocystisnoctilucarepresentsanexcellentbioassayforshearforcesinducedingroundbasedmicrogravitysimulatorsclinostatandrandompositioningmachine AT volkancevik pyrocystisnoctilucarepresentsanexcellentbioassayforshearforcesinducedingroundbasedmicrogravitysimulatorsclinostatandrandompositioningmachine AT ruthhemmersbach pyrocystisnoctilucarepresentsanexcellentbioassayforshearforcesinducedingroundbasedmicrogravitysimulatorsclinostatandrandompositioningmachine
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Pyrocystis noctiluca represents an excellent bioassay for shear forces induced in ground-based microgravity simulators (clinostat and random positioning machine)

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