RGDS-functionalized polyethylene glycol hydrogel-coated magnetic iron oxide nanoparticles enhance specific intracellular uptake by HeLa cells

Caner Nazli1, Tugba Ipek Ergenc2, Yasemin Yar1, Havva Yagci Acar1,3, Seda Kizilel1,21Graduate School of Sciences and Engineering, Ko&ccedil; University, 2Department of Chemical and Biological Engineering, College of Engineering, Ko&ccedil; University, 3Department of Chemistry, Facult...

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Autores principales:	Nazli C, Ergenc TI, Yar Y, Acar HY, Kizilel S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9cf41a96019f487aa4c88a30a814bebc
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spelling	oai:doaj.org-article:9cf41a96019f487aa4c88a30a814bebc2021-12-02T02:06:53ZRGDS-functionalized polyethylene glycol hydrogel-coated magnetic iron oxide nanoparticles enhance specific intracellular uptake by HeLa cells1176-91141178-2013https://doaj.org/article/9cf41a96019f487aa4c88a30a814bebc2012-04-01T00:00:00Zhttp://www.dovepress.com/rgds-functionalized-polyethylene-glycol-hydrogel-coated-magnetic-iron--a9730https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Caner Nazli1, Tugba Ipek Ergenc2, Yasemin Yar1, Havva Yagci Acar1,3, Seda Kizilel1,21Graduate School of Sciences and Engineering, Ko&ccedil; University, 2Department of Chemical and Biological Engineering, College of Engineering, Ko&ccedil; University, 3Department of Chemistry, Faculty of Arts and Sciences, Ko&ccedil; University, Istanbul, TurkeyAbstract: The objective of this study was to develop thin, biocompatible, and biofunctional hydrogel-coated small-sized nanoparticles that exhibit favorable stability, viability, and specific cellular uptake. This article reports the coating of magnetic iron oxide nanoparticles (MIONPs) with covalently cross-linked biofunctional polyethylene glycol (PEG) hydrogel. Silanized MIONPs were derivatized with eosin Y, and the covalently cross-linked biofunctional PEG hydrogel coating was achieved via surface-initiated photopolymerization of PEG diacrylate in aqueous solution. The thickness of the PEG hydrogel coating, between 23 and 126 nm, was tuned with laser exposure time. PEG hydrogel-coated MIONPs were further functionalized with the fibronectin-derived arginine-glycine-aspartic acid-serine (RGDS) sequence, in order to achieve a biofunctional PEG hydrogel layer around the nanoparticles. RGDS-bound PEG hydrogel-coated MIONPs showed a 17-fold higher uptake by the human cervical cancer HeLa cell line than that of amine-coated MIONPs. This novel method allows for the coating of MIONPs with nano-thin biofunctional hydrogel layers that may prevent undesirable cell and protein adhesion and may allow for cellular uptake in target tissues in a specific manner. These findings indicate that the further biofunctional PEG hydrogel coating of MIONPs is a promising platform for enhanced specific cell targeting in biomedical imaging and cancer therapy.Keywords: PEG hydrogel, surface-initiated photopolymerization, nanoparticle encapsulation, agglomerationNazli CErgenc TIYar YAcar HYKizilel SDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 1903-1920 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Nazli C Ergenc TI Yar Y Acar HY Kizilel S RGDS-functionalized polyethylene glycol hydrogel-coated magnetic iron oxide nanoparticles enhance specific intracellular uptake by HeLa cells
description	Caner Nazli1, Tugba Ipek Ergenc2, Yasemin Yar1, Havva Yagci Acar1,3, Seda Kizilel1,21Graduate School of Sciences and Engineering, Ko&ccedil; University, 2Department of Chemical and Biological Engineering, College of Engineering, Ko&ccedil; University, 3Department of Chemistry, Faculty of Arts and Sciences, Ko&ccedil; University, Istanbul, TurkeyAbstract: The objective of this study was to develop thin, biocompatible, and biofunctional hydrogel-coated small-sized nanoparticles that exhibit favorable stability, viability, and specific cellular uptake. This article reports the coating of magnetic iron oxide nanoparticles (MIONPs) with covalently cross-linked biofunctional polyethylene glycol (PEG) hydrogel. Silanized MIONPs were derivatized with eosin Y, and the covalently cross-linked biofunctional PEG hydrogel coating was achieved via surface-initiated photopolymerization of PEG diacrylate in aqueous solution. The thickness of the PEG hydrogel coating, between 23 and 126 nm, was tuned with laser exposure time. PEG hydrogel-coated MIONPs were further functionalized with the fibronectin-derived arginine-glycine-aspartic acid-serine (RGDS) sequence, in order to achieve a biofunctional PEG hydrogel layer around the nanoparticles. RGDS-bound PEG hydrogel-coated MIONPs showed a 17-fold higher uptake by the human cervical cancer HeLa cell line than that of amine-coated MIONPs. This novel method allows for the coating of MIONPs with nano-thin biofunctional hydrogel layers that may prevent undesirable cell and protein adhesion and may allow for cellular uptake in target tissues in a specific manner. These findings indicate that the further biofunctional PEG hydrogel coating of MIONPs is a promising platform for enhanced specific cell targeting in biomedical imaging and cancer therapy.Keywords: PEG hydrogel, surface-initiated photopolymerization, nanoparticle encapsulation, agglomeration
format	article
author	Nazli C Ergenc TI Yar Y Acar HY Kizilel S
author_facet	Nazli C Ergenc TI Yar Y Acar HY Kizilel S
author_sort	Nazli C
title	RGDS-functionalized polyethylene glycol hydrogel-coated magnetic iron oxide nanoparticles enhance specific intracellular uptake by HeLa cells
title_short	RGDS-functionalized polyethylene glycol hydrogel-coated magnetic iron oxide nanoparticles enhance specific intracellular uptake by HeLa cells
title_full	RGDS-functionalized polyethylene glycol hydrogel-coated magnetic iron oxide nanoparticles enhance specific intracellular uptake by HeLa cells
title_fullStr	RGDS-functionalized polyethylene glycol hydrogel-coated magnetic iron oxide nanoparticles enhance specific intracellular uptake by HeLa cells
title_full_unstemmed	RGDS-functionalized polyethylene glycol hydrogel-coated magnetic iron oxide nanoparticles enhance specific intracellular uptake by HeLa cells
title_sort	rgds-functionalized polyethylene glycol hydrogel-coated magnetic iron oxide nanoparticles enhance specific intracellular uptake by hela cells
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/9cf41a96019f487aa4c88a30a814bebc
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RGDS-functionalized polyethylene glycol hydrogel-coated magnetic iron oxide nanoparticles enhance specific intracellular uptake by HeLa cells

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