iTRAQ-Based Proteomics Analysis of Response to <i>Solanum tuberosum</i> Leaves Treated with the Plant Phytotoxin Thaxtomin A

Thaxtomin A (TA) is a phytotoxin secreted by <i>Streptomyces scabies</i> that causes common scab in potatoes. However, the mechanism of potato proteomic changes in response to TA is barely known. In this study, the proteomic changes in potato leaves treated with TA were determined using...

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Autores principales:	Lu Liu, Liaoyang Hao, Ning Liu, Yonglong Zhao, Naiqin Zhong, Pan Zhao
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Solanum tuberosum</i> common scab thaxtomin A proteomic analysis iTRAQ Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/9cf6f3cba9ae428393774bebaabd7555
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spelling	oai:doaj.org-article:9cf6f3cba9ae428393774bebaabd75552021-11-11T17:26:08ZiTRAQ-Based Proteomics Analysis of Response to <i>Solanum tuberosum</i> Leaves Treated with the Plant Phytotoxin Thaxtomin A10.3390/ijms2221120361422-00671661-6596https://doaj.org/article/9cf6f3cba9ae428393774bebaabd75552021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/12036https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Thaxtomin A (TA) is a phytotoxin secreted by <i>Streptomyces scabies</i> that causes common scab in potatoes. However, the mechanism of potato proteomic changes in response to TA is barely known. In this study, the proteomic changes in potato leaves treated with TA were determined using the Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) technique. A total of 693 proteins were considered as differentially expressed proteins (DEPs) following a comparison of leaves treated with TA and sterile water (as a control). Among the identified DEPs, 460 and 233 were upregulated and downregulated, respectively. Based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, many DEPs were found to be involved in defense and stress responses. Most DEPs were grouped in carbohydrate metabolism, amino acid metabolism, energy metabolism, and secondary metabolism including oxidation–reduction process, response to stress, plant–pathogen interaction, and plant hormone signal transduction. In this study, we analyzed the changes in proteins to elucidate the mechanism of potato response to TA, and we provided a molecular basis to further study the interaction between plant and TA. These results also offer the option for potato breeding through analysis of the resistant common scab.Lu LiuLiaoyang HaoNing LiuYonglong ZhaoNaiqin ZhongPan ZhaoMDPI AGarticle<i>Solanum tuberosum</i>common scabthaxtomin Aproteomic analysisiTRAQBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12036, p 12036 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Solanum tuberosum</i> common scab thaxtomin A proteomic analysis iTRAQ Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Solanum tuberosum</i> common scab thaxtomin A proteomic analysis iTRAQ Biology (General) QH301-705.5 Chemistry QD1-999 Lu Liu Liaoyang Hao Ning Liu Yonglong Zhao Naiqin Zhong Pan Zhao iTRAQ-Based Proteomics Analysis of Response to <i>Solanum tuberosum</i> Leaves Treated with the Plant Phytotoxin Thaxtomin A
description	Thaxtomin A (TA) is a phytotoxin secreted by <i>Streptomyces scabies</i> that causes common scab in potatoes. However, the mechanism of potato proteomic changes in response to TA is barely known. In this study, the proteomic changes in potato leaves treated with TA were determined using the Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) technique. A total of 693 proteins were considered as differentially expressed proteins (DEPs) following a comparison of leaves treated with TA and sterile water (as a control). Among the identified DEPs, 460 and 233 were upregulated and downregulated, respectively. Based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, many DEPs were found to be involved in defense and stress responses. Most DEPs were grouped in carbohydrate metabolism, amino acid metabolism, energy metabolism, and secondary metabolism including oxidation–reduction process, response to stress, plant–pathogen interaction, and plant hormone signal transduction. In this study, we analyzed the changes in proteins to elucidate the mechanism of potato response to TA, and we provided a molecular basis to further study the interaction between plant and TA. These results also offer the option for potato breeding through analysis of the resistant common scab.
format	article
author	Lu Liu Liaoyang Hao Ning Liu Yonglong Zhao Naiqin Zhong Pan Zhao
author_facet	Lu Liu Liaoyang Hao Ning Liu Yonglong Zhao Naiqin Zhong Pan Zhao
author_sort	Lu Liu
title	iTRAQ-Based Proteomics Analysis of Response to <i>Solanum tuberosum</i> Leaves Treated with the Plant Phytotoxin Thaxtomin A
title_short	iTRAQ-Based Proteomics Analysis of Response to <i>Solanum tuberosum</i> Leaves Treated with the Plant Phytotoxin Thaxtomin A
title_full	iTRAQ-Based Proteomics Analysis of Response to <i>Solanum tuberosum</i> Leaves Treated with the Plant Phytotoxin Thaxtomin A
title_fullStr	iTRAQ-Based Proteomics Analysis of Response to <i>Solanum tuberosum</i> Leaves Treated with the Plant Phytotoxin Thaxtomin A
title_full_unstemmed	iTRAQ-Based Proteomics Analysis of Response to <i>Solanum tuberosum</i> Leaves Treated with the Plant Phytotoxin Thaxtomin A
title_sort	itraq-based proteomics analysis of response to <i>solanum tuberosum</i> leaves treated with the plant phytotoxin thaxtomin a
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9cf6f3cba9ae428393774bebaabd7555
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iTRAQ-Based Proteomics Analysis of Response to <i>Solanum tuberosum</i> Leaves Treated with the Plant Phytotoxin Thaxtomin A

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