Evaluation of oxidative response and tissular damage in rat lungs exposed to silica-coated gold nanoparticles under static magnetic fields

Soumaya Ferchichi,1 Hamdi Trabelsi,1 Inès Azzouz,1 Amel Hanini,2 Ahmed Rejeb,3 Olfa Tebourbi,1 Mohsen Sakly,1 Hafedh Abdelmelek1 1Laboratory of Integrative Physiology, Faculty Of Sciences of Bizerte, 2Laboratory of Vascular Pathology, Carthage University, Carthage 3Laboratory of Patholog...

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Autores principales:	Ferchichi S, Trabelsi H, Azzouz I, Hanini A, Rejeb A, Tebourbi O, Sakly M, Abdelmelek H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	gold nanoparticles static magnetic fields lungs nanotoxicity oxidative stress. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9cf75093589941e49bf84dbaf426221f
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spelling	oai:doaj.org-article:9cf75093589941e49bf84dbaf426221f2021-12-02T05:08:59ZEvaluation of oxidative response and tissular damage in rat lungs exposed to silica-coated gold nanoparticles under static magnetic fields1178-2013https://doaj.org/article/9cf75093589941e49bf84dbaf426221f2016-06-01T00:00:00Zhttps://www.dovepress.com/evaluation-of-oxidative-response-and-tissular-damage-in-rat-lungs-expo-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Soumaya Ferchichi,1 Hamdi Trabelsi,1 Inès Azzouz,1 Amel Hanini,2 Ahmed Rejeb,3 Olfa Tebourbi,1 Mohsen Sakly,1 Hafedh Abdelmelek1 1Laboratory of Integrative Physiology, Faculty Of Sciences of Bizerte, 2Laboratory of Vascular Pathology, Carthage University, Carthage 3Laboratory of Pathological Anatomy, National School of Veterinary Medicine of Sidi Thabet, Manouba Univeristy, Manouba, Tunisia Abstract: The purpose of our study was the evaluation of toxicological effects of silica-coated gold nanoparticles (GNPs) and static magnetic fields (SMFs; 128 mT) exposure in rat lungs. Animals received a single injection of GNPs (1,100 µg/kg, 100 nm, intraperitoneally) and were exposed to SMFs, over 14 days (1 h/day). Results showed that GNPs treatment induced a hyperplasia of bronchus-associated lymphoid tissue. Fluorescence microscopy images showed that red fluorescence signal was detected in rat lungs after 2 weeks from the single injection of GNPs. Oxidative response study showed that GNPs exposure increased malondialdehyde level and decreased CuZn-superoxide dismutase, catalase, and glutathione peroxidase activities in rat lungs. Furthermore, the histopathological study showed that combined effects of GNPs and SMFs led to more tissular damages in rat lungs in comparison with GNPs-treated rats. Interestingly, intensity of red fluorescence signal was enhanced after exposure to SMFs indicating a higher accumulation of GNPs in rat lungs under magnetic environment. Moreover, rats coexposed to GNPs and SMFs showed an increased malondialdehyde level, a fall of CuZn-superoxide dismutase, catalase, and glutathione peroxidase activities in comparison with GNPs-treated group. Hence, SMFs exposure increased the accumulation of GNPs in rat lungs and led to more toxic effects of these nanocomplexes. Keywords: malondialdehyde, catalase, superoxide dismutase, glutathione peroxidase, bronchus-associated lymphoid tissue, nanotoxicity, histopathological studyFerchichi STrabelsi HAzzouz IHanini ARejeb ATebourbi OSakly MAbdelmelek HDove Medical Pressarticlegold nanoparticlesstatic magnetic fieldslungsnanotoxicityoxidative stress.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 2711-2719 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	gold nanoparticles static magnetic fields lungs nanotoxicity oxidative stress. Medicine (General) R5-920
spellingShingle	gold nanoparticles static magnetic fields lungs nanotoxicity oxidative stress. Medicine (General) R5-920 Ferchichi S Trabelsi H Azzouz I Hanini A Rejeb A Tebourbi O Sakly M Abdelmelek H Evaluation of oxidative response and tissular damage in rat lungs exposed to silica-coated gold nanoparticles under static magnetic fields
description	Soumaya Ferchichi,1 Hamdi Trabelsi,1 Inès Azzouz,1 Amel Hanini,2 Ahmed Rejeb,3 Olfa Tebourbi,1 Mohsen Sakly,1 Hafedh Abdelmelek1 1Laboratory of Integrative Physiology, Faculty Of Sciences of Bizerte, 2Laboratory of Vascular Pathology, Carthage University, Carthage 3Laboratory of Pathological Anatomy, National School of Veterinary Medicine of Sidi Thabet, Manouba Univeristy, Manouba, Tunisia Abstract: The purpose of our study was the evaluation of toxicological effects of silica-coated gold nanoparticles (GNPs) and static magnetic fields (SMFs; 128 mT) exposure in rat lungs. Animals received a single injection of GNPs (1,100 µg/kg, 100 nm, intraperitoneally) and were exposed to SMFs, over 14 days (1 h/day). Results showed that GNPs treatment induced a hyperplasia of bronchus-associated lymphoid tissue. Fluorescence microscopy images showed that red fluorescence signal was detected in rat lungs after 2 weeks from the single injection of GNPs. Oxidative response study showed that GNPs exposure increased malondialdehyde level and decreased CuZn-superoxide dismutase, catalase, and glutathione peroxidase activities in rat lungs. Furthermore, the histopathological study showed that combined effects of GNPs and SMFs led to more tissular damages in rat lungs in comparison with GNPs-treated rats. Interestingly, intensity of red fluorescence signal was enhanced after exposure to SMFs indicating a higher accumulation of GNPs in rat lungs under magnetic environment. Moreover, rats coexposed to GNPs and SMFs showed an increased malondialdehyde level, a fall of CuZn-superoxide dismutase, catalase, and glutathione peroxidase activities in comparison with GNPs-treated group. Hence, SMFs exposure increased the accumulation of GNPs in rat lungs and led to more toxic effects of these nanocomplexes. Keywords: malondialdehyde, catalase, superoxide dismutase, glutathione peroxidase, bronchus-associated lymphoid tissue, nanotoxicity, histopathological study
format	article
author	Ferchichi S Trabelsi H Azzouz I Hanini A Rejeb A Tebourbi O Sakly M Abdelmelek H
author_facet	Ferchichi S Trabelsi H Azzouz I Hanini A Rejeb A Tebourbi O Sakly M Abdelmelek H
author_sort	Ferchichi S
title	Evaluation of oxidative response and tissular damage in rat lungs exposed to silica-coated gold nanoparticles under static magnetic fields
title_short	Evaluation of oxidative response and tissular damage in rat lungs exposed to silica-coated gold nanoparticles under static magnetic fields
title_full	Evaluation of oxidative response and tissular damage in rat lungs exposed to silica-coated gold nanoparticles under static magnetic fields
title_fullStr	Evaluation of oxidative response and tissular damage in rat lungs exposed to silica-coated gold nanoparticles under static magnetic fields
title_full_unstemmed	Evaluation of oxidative response and tissular damage in rat lungs exposed to silica-coated gold nanoparticles under static magnetic fields
title_sort	evaluation of oxidative response and tissular damage in rat lungs exposed to silica-coated gold nanoparticles under static magnetic fields
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/9cf75093589941e49bf84dbaf426221f
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Evaluation of oxidative response and tissular damage in rat lungs exposed to silica-coated gold nanoparticles under static magnetic fields

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