Novel Cytoskeleton-Associated Proteins in <i>Trypanosoma brucei</i> Are Essential for Cell Morphogenesis and Cytokinesis

<i>Trypanosome brucei</i>, the causative agent of African sleeping sickness, harbours a highly ordered, subpellicular microtubule cytoskeleton that defines many aspects of morphology, motility and virulence. This array of microtubules is associated with a large number of proteins involve...

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Autores principales:	Marina Schock, Steffen Schmidt, Klaus Ersfeld
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Trypanosoma brucei</i> cytoskeleton microtubules BioID mass spectrometry Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9cf8f642db6742d796672ed76d8725e8
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spelling	oai:doaj.org-article:9cf8f642db6742d796672ed76d8725e82021-11-25T18:24:26ZNovel Cytoskeleton-Associated Proteins in <i>Trypanosoma brucei</i> Are Essential for Cell Morphogenesis and Cytokinesis10.3390/microorganisms91122342076-2607https://doaj.org/article/9cf8f642db6742d796672ed76d8725e82021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2234https://doaj.org/toc/2076-2607<i>Trypanosome brucei</i>, the causative agent of African sleeping sickness, harbours a highly ordered, subpellicular microtubule cytoskeleton that defines many aspects of morphology, motility and virulence. This array of microtubules is associated with a large number of proteins involved in its regulation. Employing proximity-dependent biotinylation assay (BioID) using the well characterised cytoskeleton-associated protein CAP5.5 as a probe, we identified CAP50 (Tb927.11.2610). This protein colocalises with the subpellicular cytoskeleton microtubules but not with the flagellum. Depletion by RNAi results in defects in cytokinesis, morphology and partial disorganisation of microtubule arrays. Published proteomics data indicate a possible association of CAP50 with two other, yet uncharacterised, cytoskeletal proteins, CAP52 (Tb927.6.5070) and CAP42 (Tb927.4.1300), which were therefore included in our analysis. We show that their depletion causes phenotypes similar to those described for CAP50 and that they are essential for cellular integrity.Marina SchockSteffen SchmidtKlaus ErsfeldMDPI AGarticle<i>Trypanosoma brucei</i>cytoskeletonmicrotubulesBioIDmass spectrometryBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2234, p 2234 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Trypanosoma brucei</i> cytoskeleton microtubules BioID mass spectrometry Biology (General) QH301-705.5
spellingShingle	<i>Trypanosoma brucei</i> cytoskeleton microtubules BioID mass spectrometry Biology (General) QH301-705.5 Marina Schock Steffen Schmidt Klaus Ersfeld Novel Cytoskeleton-Associated Proteins in <i>Trypanosoma brucei</i> Are Essential for Cell Morphogenesis and Cytokinesis
description	<i>Trypanosome brucei</i>, the causative agent of African sleeping sickness, harbours a highly ordered, subpellicular microtubule cytoskeleton that defines many aspects of morphology, motility and virulence. This array of microtubules is associated with a large number of proteins involved in its regulation. Employing proximity-dependent biotinylation assay (BioID) using the well characterised cytoskeleton-associated protein CAP5.5 as a probe, we identified CAP50 (Tb927.11.2610). This protein colocalises with the subpellicular cytoskeleton microtubules but not with the flagellum. Depletion by RNAi results in defects in cytokinesis, morphology and partial disorganisation of microtubule arrays. Published proteomics data indicate a possible association of CAP50 with two other, yet uncharacterised, cytoskeletal proteins, CAP52 (Tb927.6.5070) and CAP42 (Tb927.4.1300), which were therefore included in our analysis. We show that their depletion causes phenotypes similar to those described for CAP50 and that they are essential for cellular integrity.
format	article
author	Marina Schock Steffen Schmidt Klaus Ersfeld
author_facet	Marina Schock Steffen Schmidt Klaus Ersfeld
author_sort	Marina Schock
title	Novel Cytoskeleton-Associated Proteins in <i>Trypanosoma brucei</i> Are Essential for Cell Morphogenesis and Cytokinesis
title_short	Novel Cytoskeleton-Associated Proteins in <i>Trypanosoma brucei</i> Are Essential for Cell Morphogenesis and Cytokinesis
title_full	Novel Cytoskeleton-Associated Proteins in <i>Trypanosoma brucei</i> Are Essential for Cell Morphogenesis and Cytokinesis
title_fullStr	Novel Cytoskeleton-Associated Proteins in <i>Trypanosoma brucei</i> Are Essential for Cell Morphogenesis and Cytokinesis
title_full_unstemmed	Novel Cytoskeleton-Associated Proteins in <i>Trypanosoma brucei</i> Are Essential for Cell Morphogenesis and Cytokinesis
title_sort	novel cytoskeleton-associated proteins in <i>trypanosoma brucei</i> are essential for cell morphogenesis and cytokinesis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9cf8f642db6742d796672ed76d8725e8
work_keys_str_mv	AT marinaschock novelcytoskeletonassociatedproteinsinitrypanosomabruceiiareessentialforcellmorphogenesisandcytokinesis AT steffenschmidt novelcytoskeletonassociatedproteinsinitrypanosomabruceiiareessentialforcellmorphogenesisandcytokinesis AT klausersfeld novelcytoskeletonassociatedproteinsinitrypanosomabruceiiareessentialforcellmorphogenesisandcytokinesis
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Novel Cytoskeleton-Associated Proteins in <i>Trypanosoma brucei</i> Are Essential for Cell Morphogenesis and Cytokinesis

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