Genome Organizations and Functional Analyses of a Novel Gammapartitivirus from Rhizoctonia solani AG-1 IA Strain D122

Here, we describe a novel double-stranded (ds) RNA mycovirus designated Rhizoctonia solani dsRNA virus 5 (RsRV5) from strain D122 of Rhizoctonia solani AG-1 IA, the causal agent of rice sheath blight. The RsRV5 genome consists of two segments of dsRNA (dsRNA-1,...

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Autores principales:	Meiling Zhang, Zhenrui He, Xiaotong Huang, Canwei Shu, Erxun Zhou
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	mycovirus <i>Gammapartitivirus</i> hypovirulence <i>Rhizoctonia solani</i> transcriptome biocontrol Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/9cf91af3e7a8444594475dcf8a3a11e0
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spelling	oai:doaj.org-article:9cf91af3e7a8444594475dcf8a3a11e02021-11-25T19:13:48ZGenome Organizations and Functional Analyses of a Novel Gammapartitivirus from <i>Rhizoctonia solani</i> AG-1 IA Strain D12210.3390/v131122541999-4915https://doaj.org/article/9cf91af3e7a8444594475dcf8a3a11e02021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2254https://doaj.org/toc/1999-4915Here, we describe a novel double-stranded (ds) RNA mycovirus designated Rhizoctonia solani dsRNA virus 5 (RsRV5) from strain D122 of <i>R</i><i>hizoctonia solani</i> AG-1 IA, the causal agent of rice sheath blight. The RsRV5 genome consists of two segments of dsRNA (dsRNA-1, 1894 bp and dsRNA-2, 1755 bp), each possessing a single open reading frame (ORF). Sequence alignments and phylogenetic analyses showed that RsRV5 is a new member of the genus <i>Gammapartitivirus</i> in the family <i>Partitiviridae</i>. Transmission electron microscope (TEM) images revealed that RsRV5 has isometric viral particles with a diameter of approximately 20 nm. The mycovirus RsRV5 was successfully removed from strain D122 by using the protoplast regeneration technique, thus resulting in derivative isogenic RsRV5-cured strain D122-P being obtained. RsRV5-cured strain D122-P possessed the traits of accelerated mycelial growth rate, increased sclerotia production and enhanced pathogenicity to rice leaves compared with wild type RsRV5-infection strain D122. Transcriptome analysis showed that three genes were differentially expressed between two isogenic strains, D122 and D122-P. These findings provided new insights into the molecular mechanism of the interaction between RsRV5 and its host, D122 of <i>R. solani</i> AG-1 IA.Meiling ZhangZhenrui HeXiaotong HuangCanwei ShuErxun ZhouMDPI AGarticlemycovirus<i>Gammapartitivirus</i>hypovirulence<i>Rhizoctonia solani</i>transcriptomebiocontrolMicrobiologyQR1-502ENViruses, Vol 13, Iss 2254, p 2254 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mycovirus <i>Gammapartitivirus</i> hypovirulence <i>Rhizoctonia solani</i> transcriptome biocontrol Microbiology QR1-502
spellingShingle	mycovirus <i>Gammapartitivirus</i> hypovirulence <i>Rhizoctonia solani</i> transcriptome biocontrol Microbiology QR1-502 Meiling Zhang Zhenrui He Xiaotong Huang Canwei Shu Erxun Zhou Genome Organizations and Functional Analyses of a Novel Gammapartitivirus from <i>Rhizoctonia solani</i> AG-1 IA Strain D122
description	Here, we describe a novel double-stranded (ds) RNA mycovirus designated Rhizoctonia solani dsRNA virus 5 (RsRV5) from strain D122 of <i>R</i><i>hizoctonia solani</i> AG-1 IA, the causal agent of rice sheath blight. The RsRV5 genome consists of two segments of dsRNA (dsRNA-1, 1894 bp and dsRNA-2, 1755 bp), each possessing a single open reading frame (ORF). Sequence alignments and phylogenetic analyses showed that RsRV5 is a new member of the genus <i>Gammapartitivirus</i> in the family <i>Partitiviridae</i>. Transmission electron microscope (TEM) images revealed that RsRV5 has isometric viral particles with a diameter of approximately 20 nm. The mycovirus RsRV5 was successfully removed from strain D122 by using the protoplast regeneration technique, thus resulting in derivative isogenic RsRV5-cured strain D122-P being obtained. RsRV5-cured strain D122-P possessed the traits of accelerated mycelial growth rate, increased sclerotia production and enhanced pathogenicity to rice leaves compared with wild type RsRV5-infection strain D122. Transcriptome analysis showed that three genes were differentially expressed between two isogenic strains, D122 and D122-P. These findings provided new insights into the molecular mechanism of the interaction between RsRV5 and its host, D122 of <i>R. solani</i> AG-1 IA.
format	article
author	Meiling Zhang Zhenrui He Xiaotong Huang Canwei Shu Erxun Zhou
author_facet	Meiling Zhang Zhenrui He Xiaotong Huang Canwei Shu Erxun Zhou
author_sort	Meiling Zhang
title	Genome Organizations and Functional Analyses of a Novel Gammapartitivirus from <i>Rhizoctonia solani</i> AG-1 IA Strain D122
title_short	Genome Organizations and Functional Analyses of a Novel Gammapartitivirus from <i>Rhizoctonia solani</i> AG-1 IA Strain D122
title_full	Genome Organizations and Functional Analyses of a Novel Gammapartitivirus from <i>Rhizoctonia solani</i> AG-1 IA Strain D122
title_fullStr	Genome Organizations and Functional Analyses of a Novel Gammapartitivirus from <i>Rhizoctonia solani</i> AG-1 IA Strain D122
title_full_unstemmed	Genome Organizations and Functional Analyses of a Novel Gammapartitivirus from <i>Rhizoctonia solani</i> AG-1 IA Strain D122
title_sort	genome organizations and functional analyses of a novel gammapartitivirus from <i>rhizoctonia solani</i> ag-1 ia strain d122
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9cf91af3e7a8444594475dcf8a3a11e0
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Genome Organizations and Functional Analyses of a Novel Gammapartitivirus from <i>Rhizoctonia solani</i> AG-1 IA Strain D122

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