Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes
Abstract. Carsono N, Juwendah E, Liberty, Sari S, Damayanti F, Rachmadi M. 2021. Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes. Biodiversitas 22: 2555-2560. The development of callus in the course of transgenic rice...
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oai:doaj.org-article:9cfba91cd97645ed87c4f2d96da832dc2021-11-22T12:16:56ZOptimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes1412-033X2085-472210.13057/biodiv/d220702https://doaj.org/article/9cfba91cd97645ed87c4f2d96da832dc2021-06-01T00:00:00Zhttps://smujo.id/biodiv/article/view/8136https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Carsono N, Juwendah E, Liberty, Sari S, Damayanti F, Rachmadi M. 2021. Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes. Biodiversitas 22: 2555-2560. The development of callus in the course of transgenic rice avoids the somaclonal variants. To obtain a high number of normal phenotypes and a low number of somaclonal variants requires an appropriate 2,4-D concentration. In this study, we obtained the best callus induction time and a high number of green plant regeneration for three responsive rice genotypes on different 2,4-D concentrations in NB5 medium. The mature seeds of rice embryos were used as explants. A completely randomized factorial design was applied with four levels of 2,4-D concentrations (0, 1, 3, and 5 ppm), two levels of induction time (one and two weeks), and three rice genotypes (cv. Fatmawati, Nipponbare, and Kitaake). The study revealed that there was no interaction effect among genotype, 2,4-D concentration, and callus induction time. Three rice genotypes performed best in callus proliferation and regeneration. One-week callus induction time showed higher callus growth capacity (CGC) as compared to two-week callus induction time. Shoot regeneration capacity (SRC) was independently affected by genotype as well as by callus induction time. The interaction effect between 2,4-D concentration and callus induction time was observed on plant regeneration capacity (PRC). Without the addition of 2,4-D and 1 ppm of 2,4-D, the green plant regeneration capacity (GRC) was comparatively higher. Addition of 2,4-D showed a significant effect, especially at the plant regeneration stage. We found that one-week callus induction was the best treatment for callus proliferation and plant regeneration. We recommend the use of one-week callus induction and 1 ppm of 2,4-D for rice callus proliferation (sub-culture) and subsequent plant regeneration.Nono CarsonoENDAH JUWENDAHLIBERTY LIBERTYSANTIKA SARIFARIDA DAMAYANTIMEDDY RACHMADIMBI & UNS Soloarticlein vitro culture, somaclonal variants, sub culture, rice.Biology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 7 (2021) |
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in vitro culture, somaclonal variants, sub culture, rice. Biology (General) QH301-705.5 |
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in vitro culture, somaclonal variants, sub culture, rice. Biology (General) QH301-705.5 Nono Carsono ENDAH JUWENDAH LIBERTY LIBERTY SANTIKA SARI FARIDA DAMAYANTI MEDDY RACHMADI Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes |
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Abstract. Carsono N, Juwendah E, Liberty, Sari S, Damayanti F, Rachmadi M. 2021. Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes. Biodiversitas 22: 2555-2560. The development of callus in the course of transgenic rice avoids the somaclonal variants. To obtain a high number of normal phenotypes and a low number of somaclonal variants requires an appropriate 2,4-D concentration. In this study, we obtained the best callus induction time and a high number of green plant regeneration for three responsive rice genotypes on different 2,4-D concentrations in NB5 medium. The mature seeds of rice embryos were used as explants. A completely randomized factorial design was applied with four levels of 2,4-D concentrations (0, 1, 3, and 5 ppm), two levels of induction time (one and two weeks), and three rice genotypes (cv. Fatmawati, Nipponbare, and Kitaake). The study revealed that there was no interaction effect among genotype, 2,4-D concentration, and callus induction time. Three rice genotypes performed best in callus proliferation and regeneration. One-week callus induction time showed higher callus growth capacity (CGC) as compared to two-week callus induction time. Shoot regeneration capacity (SRC) was independently affected by genotype as well as by callus induction time. The interaction effect between 2,4-D concentration and callus induction time was observed on plant regeneration capacity (PRC). Without the addition of 2,4-D and 1 ppm of 2,4-D, the green plant regeneration capacity (GRC) was comparatively higher. Addition of 2,4-D showed a significant effect, especially at the plant regeneration stage. We found that one-week callus induction was the best treatment for callus proliferation and plant regeneration. We recommend the use of one-week callus induction and 1 ppm of 2,4-D for rice callus proliferation (sub-culture) and subsequent plant regeneration. |
format |
article |
author |
Nono Carsono ENDAH JUWENDAH LIBERTY LIBERTY SANTIKA SARI FARIDA DAMAYANTI MEDDY RACHMADI |
author_facet |
Nono Carsono ENDAH JUWENDAH LIBERTY LIBERTY SANTIKA SARI FARIDA DAMAYANTI MEDDY RACHMADI |
author_sort |
Nono Carsono |
title |
Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes |
title_short |
Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes |
title_full |
Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes |
title_fullStr |
Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes |
title_full_unstemmed |
Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes |
title_sort |
optimize 2,4-d concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes |
publisher |
MBI & UNS Solo |
publishDate |
2021 |
url |
https://doaj.org/article/9cfba91cd97645ed87c4f2d96da832dc |
work_keys_str_mv |
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