Luteolin, a natural flavonoid, inhibits methylglyoxal induced apoptosis via the mTOR/4E-BP1 signaling pathway

Abstract Methylglyoxal (MG) accumulation has been observed in human cerebrospinal fluid and body tissues under hyperglycaemic conditions. Recent research has demonstrated that MG-induces neuronal cell apoptosis, which promotes the development of diabetic encephalopathy. Our previous animal study has...

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Autores principales:	Yi Liu, Jie Huang, Xian Zheng, Xia Yang, Yan Ding, Tongyong Fang, Yuyun Zhang, Shuaishuai Wang, Xiaofei Zhang, Xuan Luo, Anlei Guo, Kelly A. Newell, Yinghua Yu, Xu-Feng Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9cfd609f8aa54a7884e456f962e96767
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spelling	oai:doaj.org-article:9cfd609f8aa54a7884e456f962e967672021-12-02T11:51:13ZLuteolin, a natural flavonoid, inhibits methylglyoxal induced apoptosis via the mTOR/4E-BP1 signaling pathway10.1038/s41598-017-08204-62045-2322https://doaj.org/article/9cfd609f8aa54a7884e456f962e967672017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08204-6https://doaj.org/toc/2045-2322Abstract Methylglyoxal (MG) accumulation has been observed in human cerebrospinal fluid and body tissues under hyperglycaemic conditions. Recent research has demonstrated that MG-induces neuronal cell apoptosis, which promotes the development of diabetic encephalopathy. Our previous animal study has shown that luteolin, a natural flavonoid, attenuates diabetes-associated cognitive dysfunction. To further explore the neuroprotective properties of luteolin, we investigated the inhibitive effect of luteolin on MG-induced apoptosis in PC12 neuronal cells. We found that MG inhibited cell viability in a dose-dependent manner and induced apoptosis in PC12 cells. Pretreatment with Luteolin significantly elevated cell viability, reduced MG-induced apoptosis, inhibited the activation of the mTOR/4E-BP1 signaling pathway, and decreased pro-apoptotic proteins, Bax, Cytochrome C as well as caspase-3. Furthermore, we found that pretreatment with the mTOR inhibitor, rapamycin, significantly reduced the expression of the pro-apoptotic protein Bax. Therefore, these observations unambiguously suggest that the inhibitive effect of Luteolin against MG-induced apoptosis in PC12 cells is associated with inhibition of the mTOR/4E-BP1 signaling pathway.Yi LiuJie HuangXian ZhengXia YangYan DingTongyong FangYuyun ZhangShuaishuai WangXiaofei ZhangXuan LuoAnlei GuoKelly A. NewellYinghua YuXu-Feng HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yi Liu Jie Huang Xian Zheng Xia Yang Yan Ding Tongyong Fang Yuyun Zhang Shuaishuai Wang Xiaofei Zhang Xuan Luo Anlei Guo Kelly A. Newell Yinghua Yu Xu-Feng Huang Luteolin, a natural flavonoid, inhibits methylglyoxal induced apoptosis via the mTOR/4E-BP1 signaling pathway
description	Abstract Methylglyoxal (MG) accumulation has been observed in human cerebrospinal fluid and body tissues under hyperglycaemic conditions. Recent research has demonstrated that MG-induces neuronal cell apoptosis, which promotes the development of diabetic encephalopathy. Our previous animal study has shown that luteolin, a natural flavonoid, attenuates diabetes-associated cognitive dysfunction. To further explore the neuroprotective properties of luteolin, we investigated the inhibitive effect of luteolin on MG-induced apoptosis in PC12 neuronal cells. We found that MG inhibited cell viability in a dose-dependent manner and induced apoptosis in PC12 cells. Pretreatment with Luteolin significantly elevated cell viability, reduced MG-induced apoptosis, inhibited the activation of the mTOR/4E-BP1 signaling pathway, and decreased pro-apoptotic proteins, Bax, Cytochrome C as well as caspase-3. Furthermore, we found that pretreatment with the mTOR inhibitor, rapamycin, significantly reduced the expression of the pro-apoptotic protein Bax. Therefore, these observations unambiguously suggest that the inhibitive effect of Luteolin against MG-induced apoptosis in PC12 cells is associated with inhibition of the mTOR/4E-BP1 signaling pathway.
format	article
author	Yi Liu Jie Huang Xian Zheng Xia Yang Yan Ding Tongyong Fang Yuyun Zhang Shuaishuai Wang Xiaofei Zhang Xuan Luo Anlei Guo Kelly A. Newell Yinghua Yu Xu-Feng Huang
author_facet	Yi Liu Jie Huang Xian Zheng Xia Yang Yan Ding Tongyong Fang Yuyun Zhang Shuaishuai Wang Xiaofei Zhang Xuan Luo Anlei Guo Kelly A. Newell Yinghua Yu Xu-Feng Huang
author_sort	Yi Liu
title	Luteolin, a natural flavonoid, inhibits methylglyoxal induced apoptosis via the mTOR/4E-BP1 signaling pathway
title_short	Luteolin, a natural flavonoid, inhibits methylglyoxal induced apoptosis via the mTOR/4E-BP1 signaling pathway
title_full	Luteolin, a natural flavonoid, inhibits methylglyoxal induced apoptosis via the mTOR/4E-BP1 signaling pathway
title_fullStr	Luteolin, a natural flavonoid, inhibits methylglyoxal induced apoptosis via the mTOR/4E-BP1 signaling pathway
title_full_unstemmed	Luteolin, a natural flavonoid, inhibits methylglyoxal induced apoptosis via the mTOR/4E-BP1 signaling pathway
title_sort	luteolin, a natural flavonoid, inhibits methylglyoxal induced apoptosis via the mtor/4e-bp1 signaling pathway
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9cfd609f8aa54a7884e456f962e96767
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Luteolin, a natural flavonoid, inhibits methylglyoxal induced apoptosis via the mTOR/4E-BP1 signaling pathway

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