Using mutability landscapes of a promiscuous tautomerase to guide the engineering of enantioselective Michaelases

The Michael-type addition reaction is used for carbon-carbon bond formation; however biocatalytic methods for this reaction are rare. Here, the authors generate and exploit mutability landscapes of 4-oxalocrotonate tautomerase to direct the redesign of this promiscuous enzyme into enantio-complement...

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Autores principales:	Jan-Ytzen van der Meer, Harshwardhan Poddar, Bert-Jan Baas, Yufeng Miao, Mehran Rahimi, Andreas Kunzendorf, Ronald van Merkerk, Pieter G. Tepper, Edzard M. Geertsema, Andy-Mark W. H. Thunnissen, Wim J. Quax, Gerrit J. Poelarends
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/9cfd7194e093495a81ae07646995d9a2
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spelling	oai:doaj.org-article:9cfd7194e093495a81ae07646995d9a22021-12-02T17:31:49ZUsing mutability landscapes of a promiscuous tautomerase to guide the engineering of enantioselective Michaelases10.1038/ncomms109112041-1723https://doaj.org/article/9cfd7194e093495a81ae07646995d9a22016-03-01T00:00:00Zhttps://doi.org/10.1038/ncomms10911https://doaj.org/toc/2041-1723The Michael-type addition reaction is used for carbon-carbon bond formation; however biocatalytic methods for this reaction are rare. Here, the authors generate and exploit mutability landscapes of 4-oxalocrotonate tautomerase to direct the redesign of this promiscuous enzyme into enantio-complementary Michaelases.Jan-Ytzen van der MeerHarshwardhan PoddarBert-Jan BaasYufeng MiaoMehran RahimiAndreas KunzendorfRonald van MerkerkPieter G. TepperEdzard M. GeertsemaAndy-Mark W. H. ThunnissenWim J. QuaxGerrit J. PoelarendsNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-16 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jan-Ytzen van der Meer Harshwardhan Poddar Bert-Jan Baas Yufeng Miao Mehran Rahimi Andreas Kunzendorf Ronald van Merkerk Pieter G. Tepper Edzard M. Geertsema Andy-Mark W. H. Thunnissen Wim J. Quax Gerrit J. Poelarends Using mutability landscapes of a promiscuous tautomerase to guide the engineering of enantioselective Michaelases
description	The Michael-type addition reaction is used for carbon-carbon bond formation; however biocatalytic methods for this reaction are rare. Here, the authors generate and exploit mutability landscapes of 4-oxalocrotonate tautomerase to direct the redesign of this promiscuous enzyme into enantio-complementary Michaelases.
format	article
author	Jan-Ytzen van der Meer Harshwardhan Poddar Bert-Jan Baas Yufeng Miao Mehran Rahimi Andreas Kunzendorf Ronald van Merkerk Pieter G. Tepper Edzard M. Geertsema Andy-Mark W. H. Thunnissen Wim J. Quax Gerrit J. Poelarends
author_facet	Jan-Ytzen van der Meer Harshwardhan Poddar Bert-Jan Baas Yufeng Miao Mehran Rahimi Andreas Kunzendorf Ronald van Merkerk Pieter G. Tepper Edzard M. Geertsema Andy-Mark W. H. Thunnissen Wim J. Quax Gerrit J. Poelarends
author_sort	Jan-Ytzen van der Meer
title	Using mutability landscapes of a promiscuous tautomerase to guide the engineering of enantioselective Michaelases
title_short	Using mutability landscapes of a promiscuous tautomerase to guide the engineering of enantioselective Michaelases
title_full	Using mutability landscapes of a promiscuous tautomerase to guide the engineering of enantioselective Michaelases
title_fullStr	Using mutability landscapes of a promiscuous tautomerase to guide the engineering of enantioselective Michaelases
title_full_unstemmed	Using mutability landscapes of a promiscuous tautomerase to guide the engineering of enantioselective Michaelases
title_sort	using mutability landscapes of a promiscuous tautomerase to guide the engineering of enantioselective michaelases
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/9cfd7194e093495a81ae07646995d9a2
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Using mutability landscapes of a promiscuous tautomerase to guide the engineering of enantioselective Michaelases

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