Location and level of Etk expression in neurons are associated with varied severity of traumatic brain injury.

<h4>Background</h4>Much recent research effort in traumatic brain injury (TBI) has been devoted to the discovery of a reliable biomarker correlating with severity of injury. Currently, no consensus has been reached regarding a representative marker for traumatic brain injury. In this stu...

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Autores principales:	John Chung-Che Wu, Kai-Yun Chen, Yu-Wen Yu, Song-Wei Huang, Hsiu-Ming Shih, Wen-Ta Chiu, Yung-Hsiao Chiang, Chia-Yang Shiau
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9cfe0a01ea4a48318b21378619c8350a
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spelling	oai:doaj.org-article:9cfe0a01ea4a48318b21378619c8350a2021-11-18T07:15:07ZLocation and level of Etk expression in neurons are associated with varied severity of traumatic brain injury.1932-620310.1371/journal.pone.0039226https://doaj.org/article/9cfe0a01ea4a48318b21378619c8350a2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22723969/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Much recent research effort in traumatic brain injury (TBI) has been devoted to the discovery of a reliable biomarker correlating with severity of injury. Currently, no consensus has been reached regarding a representative marker for traumatic brain injury. In this study, we explored the potential of epithelial/endothelial tyrosine kinase (Etk) as a novel marker for TBI.<h4>Methodology/principal findings</h4>TBI was induced in Sprague Dawley (SD) rats by controlled cortical impact. Brain tissue samples were analyzed by Western blot, Q-PCR, and immunofluorescence staining using various markers including glial fibrillary acidic protein, and epithelial/endothelial tyrosine kinase (Etk). Results show increased Etk expression with increased number and severity of impacts. Expression increased 2.36 to 7-fold relative to trauma severity. Significant upregulation of Etk appeared at 1 hour after injury. The expression level of Etk was inversely correlated with distance from injury site. Etk and trauma/inflammation related markers increased post-TBI, while other tyrosine kinases did not.<h4>Conclusion/significance</h4>The observed correlation between Etk level and the number of impacts, the severity of impact, and the time course after impact, as well as its inverse correlation with distance away from injury site, support the potential of Etk as a possible indicator of trauma severity.John Chung-Che WuKai-Yun ChenYu-Wen YuSong-Wei HuangHsiu-Ming ShihWen-Ta ChiuYung-Hsiao ChiangChia-Yang ShiauPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e39226 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q John Chung-Che Wu Kai-Yun Chen Yu-Wen Yu Song-Wei Huang Hsiu-Ming Shih Wen-Ta Chiu Yung-Hsiao Chiang Chia-Yang Shiau Location and level of Etk expression in neurons are associated with varied severity of traumatic brain injury.
description	<h4>Background</h4>Much recent research effort in traumatic brain injury (TBI) has been devoted to the discovery of a reliable biomarker correlating with severity of injury. Currently, no consensus has been reached regarding a representative marker for traumatic brain injury. In this study, we explored the potential of epithelial/endothelial tyrosine kinase (Etk) as a novel marker for TBI.<h4>Methodology/principal findings</h4>TBI was induced in Sprague Dawley (SD) rats by controlled cortical impact. Brain tissue samples were analyzed by Western blot, Q-PCR, and immunofluorescence staining using various markers including glial fibrillary acidic protein, and epithelial/endothelial tyrosine kinase (Etk). Results show increased Etk expression with increased number and severity of impacts. Expression increased 2.36 to 7-fold relative to trauma severity. Significant upregulation of Etk appeared at 1 hour after injury. The expression level of Etk was inversely correlated with distance from injury site. Etk and trauma/inflammation related markers increased post-TBI, while other tyrosine kinases did not.<h4>Conclusion/significance</h4>The observed correlation between Etk level and the number of impacts, the severity of impact, and the time course after impact, as well as its inverse correlation with distance away from injury site, support the potential of Etk as a possible indicator of trauma severity.
format	article
author	John Chung-Che Wu Kai-Yun Chen Yu-Wen Yu Song-Wei Huang Hsiu-Ming Shih Wen-Ta Chiu Yung-Hsiao Chiang Chia-Yang Shiau
author_facet	John Chung-Che Wu Kai-Yun Chen Yu-Wen Yu Song-Wei Huang Hsiu-Ming Shih Wen-Ta Chiu Yung-Hsiao Chiang Chia-Yang Shiau
author_sort	John Chung-Che Wu
title	Location and level of Etk expression in neurons are associated with varied severity of traumatic brain injury.
title_short	Location and level of Etk expression in neurons are associated with varied severity of traumatic brain injury.
title_full	Location and level of Etk expression in neurons are associated with varied severity of traumatic brain injury.
title_fullStr	Location and level of Etk expression in neurons are associated with varied severity of traumatic brain injury.
title_full_unstemmed	Location and level of Etk expression in neurons are associated with varied severity of traumatic brain injury.
title_sort	location and level of etk expression in neurons are associated with varied severity of traumatic brain injury.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/9cfe0a01ea4a48318b21378619c8350a
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Location and level of Etk expression in neurons are associated with varied severity of traumatic brain injury.

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