Association Between Body Mass Index and Male Sperm Apoptosis and Apoptosis-Related Factors

Guangli Zhu, Yuting Zhang, Jianjun Dong, Yilin Liu, Fang Zhao, Ting Li, Zhanrong Shi, Yanping Zhang, Na Song, Wenyue Song, Yuzhen Lv Reproductive Medicine Center, Jiaozuo Women and Children’s Hospital, Jiaozuo, Henan Province, People’s Republic of ChinaCorrespondence: Yuzhen LvRe...

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Autores principales:	Zhu G, Zhang Y, Dong J, Liu Y, Zhao F, Li T, Shi Z, Song N, Song W, Lv Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	bmi obesity semen quality sperm dna apoptosis-related factors biomarker Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/9cff0e2cbd8d45a6a6999e05e02b8f2d
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spelling	oai:doaj.org-article:9cff0e2cbd8d45a6a6999e05e02b8f2d2021-12-02T14:20:30ZAssociation Between Body Mass Index and Male Sperm Apoptosis and Apoptosis-Related Factors1178-7007https://doaj.org/article/9cff0e2cbd8d45a6a6999e05e02b8f2d2021-03-01T00:00:00Zhttps://www.dovepress.com/association-between-body-mass-index-and-male-sperm-apoptosis-and-apopt-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Guangli Zhu, Yuting Zhang, Jianjun Dong, Yilin Liu, Fang Zhao, Ting Li, Zhanrong Shi, Yanping Zhang, Na Song, Wenyue Song, Yuzhen Lv Reproductive Medicine Center, Jiaozuo Women and Children’s Hospital, Jiaozuo, Henan Province, People’s Republic of ChinaCorrespondence: Yuzhen LvReproductive Medicine Center, Jiaozuo Women and Children’s Hospital, Jiaozuo, 454150, Henan, People’s Republic of ChinaTel +8613839158938Email lyz jiaozuo@126.comBackground: Although obesity may affect reproductive functions, the molecular mechanisms of apoptosis-related biomarkers remain uncertain.Objective: To examine the effects of body mass index on sperm quality and apoptosis-related factors in seminal plasma of men.Methods: Data for 54 subfertile men were collected at our reproductive medical center. The men were divided into normal weight, overweight, and obese groups based on their body mass index (BMI). Sperm DNA fragmentation (sperm chromatin structure analysis), sperm apoptosis (annexin V), and sperm apoptosis-related factors (antibody array assay) were assessed and their relationships with BMI were analyzed.Results: BMI was not significantly related to age, duration of infertility, duration of sexual abstinence, semen volume, sperm concentration, or rate of normal sperm morphology (p > 0.05). However, progressive sperm motility was significantly reduced and the rates of sperm DNA fragmentation index (DFI) and sperm apoptosis were significantly increased in overweight and obese men compared with men with normal BMI. Fas/Fasl, Bcl-2/Bax, caspase-3, caspase-8, p53, and p21 were all upregulated in the overweight and obese groups. Protein function annotation by Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that apoptosis-related factors were enriched in a network associated with activation of apoptotic signaling pathways, such as apoptosis and p53 signaling.Conclusion: These data suggest that increased BMI is associated with increased sperm apoptosis and sperm DNA damage, as well as accelerated expression of apoptosis-related factors via the activation of apoptotic signaling pathways.Keywords: BMI, obesity, semen quality, sperm DNA, apoptosis-related factors, biomarkerZhu GZhang YDong JLiu YZhao FLi TShi ZZhang YSong NSong WLv YDove Medical Pressarticlebmiobesitysemen qualitysperm dnaapoptosis-related factorsbiomarkerSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 14, Pp 1043-1051 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bmi obesity semen quality sperm dna apoptosis-related factors biomarker Specialties of internal medicine RC581-951
spellingShingle	bmi obesity semen quality sperm dna apoptosis-related factors biomarker Specialties of internal medicine RC581-951 Zhu G Zhang Y Dong J Liu Y Zhao F Li T Shi Z Zhang Y Song N Song W Lv Y Association Between Body Mass Index and Male Sperm Apoptosis and Apoptosis-Related Factors
description	Guangli Zhu, Yuting Zhang, Jianjun Dong, Yilin Liu, Fang Zhao, Ting Li, Zhanrong Shi, Yanping Zhang, Na Song, Wenyue Song, Yuzhen Lv Reproductive Medicine Center, Jiaozuo Women and Children’s Hospital, Jiaozuo, Henan Province, People’s Republic of ChinaCorrespondence: Yuzhen LvReproductive Medicine Center, Jiaozuo Women and Children’s Hospital, Jiaozuo, 454150, Henan, People’s Republic of ChinaTel +8613839158938Email lyz jiaozuo@126.comBackground: Although obesity may affect reproductive functions, the molecular mechanisms of apoptosis-related biomarkers remain uncertain.Objective: To examine the effects of body mass index on sperm quality and apoptosis-related factors in seminal plasma of men.Methods: Data for 54 subfertile men were collected at our reproductive medical center. The men were divided into normal weight, overweight, and obese groups based on their body mass index (BMI). Sperm DNA fragmentation (sperm chromatin structure analysis), sperm apoptosis (annexin V), and sperm apoptosis-related factors (antibody array assay) were assessed and their relationships with BMI were analyzed.Results: BMI was not significantly related to age, duration of infertility, duration of sexual abstinence, semen volume, sperm concentration, or rate of normal sperm morphology (p > 0.05). However, progressive sperm motility was significantly reduced and the rates of sperm DNA fragmentation index (DFI) and sperm apoptosis were significantly increased in overweight and obese men compared with men with normal BMI. Fas/Fasl, Bcl-2/Bax, caspase-3, caspase-8, p53, and p21 were all upregulated in the overweight and obese groups. Protein function annotation by Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that apoptosis-related factors were enriched in a network associated with activation of apoptotic signaling pathways, such as apoptosis and p53 signaling.Conclusion: These data suggest that increased BMI is associated with increased sperm apoptosis and sperm DNA damage, as well as accelerated expression of apoptosis-related factors via the activation of apoptotic signaling pathways.Keywords: BMI, obesity, semen quality, sperm DNA, apoptosis-related factors, biomarker
format	article
author	Zhu G Zhang Y Dong J Liu Y Zhao F Li T Shi Z Zhang Y Song N Song W Lv Y
author_facet	Zhu G Zhang Y Dong J Liu Y Zhao F Li T Shi Z Zhang Y Song N Song W Lv Y
author_sort	Zhu G
title	Association Between Body Mass Index and Male Sperm Apoptosis and Apoptosis-Related Factors
title_short	Association Between Body Mass Index and Male Sperm Apoptosis and Apoptosis-Related Factors
title_full	Association Between Body Mass Index and Male Sperm Apoptosis and Apoptosis-Related Factors
title_fullStr	Association Between Body Mass Index and Male Sperm Apoptosis and Apoptosis-Related Factors
title_full_unstemmed	Association Between Body Mass Index and Male Sperm Apoptosis and Apoptosis-Related Factors
title_sort	association between body mass index and male sperm apoptosis and apoptosis-related factors
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/9cff0e2cbd8d45a6a6999e05e02b8f2d
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Association Between Body Mass Index and Male Sperm Apoptosis and Apoptosis-Related Factors

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