A Study Based on Metabolomics, Network Pharmacology, and Experimental Verification to Explore the Mechanism of Qinbaiqingfei Concentrated Pills in the treatment of Mycoplasma Pneumonia
Qinbaiqingfei concentrated pills (QB) are a commonly used medicine for the treatment of mycoplasma pneumonia in China, and the mechanism of action of QB needs to be studied further. Therefore, we use a combination of metabolomics and network pharmacology to clarify the mechanism of QB. Nontarget met...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:9d0a9c14ce9d4ea1b5eba443ad3afb922021-11-04T05:47:12ZA Study Based on Metabolomics, Network Pharmacology, and Experimental Verification to Explore the Mechanism of Qinbaiqingfei Concentrated Pills in the treatment of Mycoplasma Pneumonia1663-981210.3389/fphar.2021.761883https://doaj.org/article/9d0a9c14ce9d4ea1b5eba443ad3afb922021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.761883/fullhttps://doaj.org/toc/1663-9812Qinbaiqingfei concentrated pills (QB) are a commonly used medicine for the treatment of mycoplasma pneumonia in China, and the mechanism of action of QB needs to be studied further. Therefore, we use a combination of metabolomics and network pharmacology to clarify the mechanism of QB. Nontarget metabolomics studies were performed on rat serum, urine, and lung tissues, and 56 therapeutic biomarkers were found. Subsequently, the components of QB absorbed into the blood and lung tissues were clarified, and based on this finding, the core target of network pharmacology was predicted. The enrichment analysis of biomarkers–genes finally confirmed their close relationship with the NF-κB signaling pathway. By western blotting expression of the proteins in the lung tissue–related signaling pathways, it is finally confirmed that QB inhibits the NF-κB signaling pathway through SIRT1, IL-10 and MMP9, CTNNB1, EGFR, and other targets. It plays a role in regulating immunity, regulating metabolism, and treating diseases.Zheng LiuJin-hai HuoWen-ting DongGuo-dong SunFeng-jin LiYa-nan ZhangZhi-wei QinJiang pengnaWei-ming WangFrontiers Media S.A.articleQinbaiqingfei concentrated pillsmetabolomicsnetwork pharmacologyNF-κB signaling pathwaymycoplasma pneumoniaTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Qinbaiqingfei concentrated pills metabolomics network pharmacology NF-κB signaling pathway mycoplasma pneumonia Therapeutics. Pharmacology RM1-950 |
| spellingShingle |
Qinbaiqingfei concentrated pills metabolomics network pharmacology NF-κB signaling pathway mycoplasma pneumonia Therapeutics. Pharmacology RM1-950 Zheng Liu Jin-hai Huo Wen-ting Dong Guo-dong Sun Feng-jin Li Ya-nan Zhang Zhi-wei Qin Jiang pengna Wei-ming Wang A Study Based on Metabolomics, Network Pharmacology, and Experimental Verification to Explore the Mechanism of Qinbaiqingfei Concentrated Pills in the treatment of Mycoplasma Pneumonia |
| description |
Qinbaiqingfei concentrated pills (QB) are a commonly used medicine for the treatment of mycoplasma pneumonia in China, and the mechanism of action of QB needs to be studied further. Therefore, we use a combination of metabolomics and network pharmacology to clarify the mechanism of QB. Nontarget metabolomics studies were performed on rat serum, urine, and lung tissues, and 56 therapeutic biomarkers were found. Subsequently, the components of QB absorbed into the blood and lung tissues were clarified, and based on this finding, the core target of network pharmacology was predicted. The enrichment analysis of biomarkers–genes finally confirmed their close relationship with the NF-κB signaling pathway. By western blotting expression of the proteins in the lung tissue–related signaling pathways, it is finally confirmed that QB inhibits the NF-κB signaling pathway through SIRT1, IL-10 and MMP9, CTNNB1, EGFR, and other targets. It plays a role in regulating immunity, regulating metabolism, and treating diseases. |
| format |
article |
| author |
Zheng Liu Jin-hai Huo Wen-ting Dong Guo-dong Sun Feng-jin Li Ya-nan Zhang Zhi-wei Qin Jiang pengna Wei-ming Wang |
| author_facet |
Zheng Liu Jin-hai Huo Wen-ting Dong Guo-dong Sun Feng-jin Li Ya-nan Zhang Zhi-wei Qin Jiang pengna Wei-ming Wang |
| author_sort |
Zheng Liu |
| title |
A Study Based on Metabolomics, Network Pharmacology, and Experimental Verification to Explore the Mechanism of Qinbaiqingfei Concentrated Pills in the treatment of Mycoplasma Pneumonia |
| title_short |
A Study Based on Metabolomics, Network Pharmacology, and Experimental Verification to Explore the Mechanism of Qinbaiqingfei Concentrated Pills in the treatment of Mycoplasma Pneumonia |
| title_full |
A Study Based on Metabolomics, Network Pharmacology, and Experimental Verification to Explore the Mechanism of Qinbaiqingfei Concentrated Pills in the treatment of Mycoplasma Pneumonia |
| title_fullStr |
A Study Based on Metabolomics, Network Pharmacology, and Experimental Verification to Explore the Mechanism of Qinbaiqingfei Concentrated Pills in the treatment of Mycoplasma Pneumonia |
| title_full_unstemmed |
A Study Based on Metabolomics, Network Pharmacology, and Experimental Verification to Explore the Mechanism of Qinbaiqingfei Concentrated Pills in the treatment of Mycoplasma Pneumonia |
| title_sort |
study based on metabolomics, network pharmacology, and experimental verification to explore the mechanism of qinbaiqingfei concentrated pills in the treatment of mycoplasma pneumonia |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/9d0a9c14ce9d4ea1b5eba443ad3afb92 |
| work_keys_str_mv |
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