High Osmolarity Modulates Bacterial Cell Size through Reducing Initiation Volume in <named-content content-type="genus-species">Escherichia coli</named-content>

ABSTRACT Bacterial cell size is closely associated with biomass growth and cell cycle progression, including chromosome replication and cell division. It is generally proposed that Escherichia coli cells tightly control the timing of chromosome replication through maintaining a constant cell volume...

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Autores principales:	Xiongfeng Dai, Manlu Zhu
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	DnaA cell cycle cell size hyperosmotic stress initiation volume Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/9d0f6544dcdd4182a06c5bcade0ee821
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spelling	oai:doaj.org-article:9d0f6544dcdd4182a06c5bcade0ee8212021-11-15T15:22:26ZHigh Osmolarity Modulates Bacterial Cell Size through Reducing Initiation Volume in <named-content content-type="genus-species">Escherichia coli</named-content>10.1128/mSphere.00430-182379-5042https://doaj.org/article/9d0f6544dcdd4182a06c5bcade0ee8212018-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00430-18https://doaj.org/toc/2379-5042ABSTRACT Bacterial cell size is closely associated with biomass growth and cell cycle progression, including chromosome replication and cell division. It is generally proposed that Escherichia coli cells tightly control the timing of chromosome replication through maintaining a constant cell volume per origin upon initiating chromosome replication (constant initiation volume) under various growth conditions. Here, we quantitatively characterize the cell size and cell cycle of Escherichia coli cells growing exponentially under hyperosmotic stress, which is a common environmental stressor that profoundly affects the bacterial water content. The bacterial cell size is reduced by hyperosmotic stress, even though the C and D periods are remarkably prolonged, indicating a significantly reduced initiation volume. The reduced initiation volume originates from the higher concentration of DnaA initiator protein caused by water loss at high osmolarity. Our study shows suggests a fundamental role of water content in regulating bacterial cell size and has also revealed a new role of the DnaA protein in regulating the chromosome replication elongation beyond regulating the replication initiation process. IMPORTANCE Bacterial cell size depends on growth rate, cell cycle progression, and the cell volume per origin upon initiating chromosome replication (initiation volume). Here, we perform the first systematic and quantitative study of the effect of hyperosmotic stress on the E. coli cell size and cell cycle. We find that hyperosmotic stress significantly reduces the initiation volume. The reduced initiation volume is attributed to the increased DnaA concentration caused by water loss at high osmolarity, indicating a fundamental role of water content in cell size and cell cycle regulation.Xiongfeng DaiManlu ZhuAmerican Society for MicrobiologyarticleDnaAcell cyclecell sizehyperosmotic stressinitiation volumeMicrobiologyQR1-502ENmSphere, Vol 3, Iss 5 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	DnaA cell cycle cell size hyperosmotic stress initiation volume Microbiology QR1-502
spellingShingle	DnaA cell cycle cell size hyperosmotic stress initiation volume Microbiology QR1-502 Xiongfeng Dai Manlu Zhu High Osmolarity Modulates Bacterial Cell Size through Reducing Initiation Volume in <named-content content-type="genus-species">Escherichia coli</named-content>
description	ABSTRACT Bacterial cell size is closely associated with biomass growth and cell cycle progression, including chromosome replication and cell division. It is generally proposed that Escherichia coli cells tightly control the timing of chromosome replication through maintaining a constant cell volume per origin upon initiating chromosome replication (constant initiation volume) under various growth conditions. Here, we quantitatively characterize the cell size and cell cycle of Escherichia coli cells growing exponentially under hyperosmotic stress, which is a common environmental stressor that profoundly affects the bacterial water content. The bacterial cell size is reduced by hyperosmotic stress, even though the C and D periods are remarkably prolonged, indicating a significantly reduced initiation volume. The reduced initiation volume originates from the higher concentration of DnaA initiator protein caused by water loss at high osmolarity. Our study shows suggests a fundamental role of water content in regulating bacterial cell size and has also revealed a new role of the DnaA protein in regulating the chromosome replication elongation beyond regulating the replication initiation process. IMPORTANCE Bacterial cell size depends on growth rate, cell cycle progression, and the cell volume per origin upon initiating chromosome replication (initiation volume). Here, we perform the first systematic and quantitative study of the effect of hyperosmotic stress on the E. coli cell size and cell cycle. We find that hyperosmotic stress significantly reduces the initiation volume. The reduced initiation volume is attributed to the increased DnaA concentration caused by water loss at high osmolarity, indicating a fundamental role of water content in cell size and cell cycle regulation.
format	article
author	Xiongfeng Dai Manlu Zhu
author_facet	Xiongfeng Dai Manlu Zhu
author_sort	Xiongfeng Dai
title	High Osmolarity Modulates Bacterial Cell Size through Reducing Initiation Volume in <named-content content-type="genus-species">Escherichia coli</named-content>
title_short	High Osmolarity Modulates Bacterial Cell Size through Reducing Initiation Volume in <named-content content-type="genus-species">Escherichia coli</named-content>
title_full	High Osmolarity Modulates Bacterial Cell Size through Reducing Initiation Volume in <named-content content-type="genus-species">Escherichia coli</named-content>
title_fullStr	High Osmolarity Modulates Bacterial Cell Size through Reducing Initiation Volume in <named-content content-type="genus-species">Escherichia coli</named-content>
title_full_unstemmed	High Osmolarity Modulates Bacterial Cell Size through Reducing Initiation Volume in <named-content content-type="genus-species">Escherichia coli</named-content>
title_sort	high osmolarity modulates bacterial cell size through reducing initiation volume in <named-content content-type="genus-species">escherichia coli</named-content>
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/9d0f6544dcdd4182a06c5bcade0ee821
work_keys_str_mv	AT xiongfengdai highosmolaritymodulatesbacterialcellsizethroughreducinginitiationvolumeinnamedcontentcontenttypegenusspeciesescherichiacolinamedcontent AT manluzhu highosmolaritymodulatesbacterialcellsizethroughreducinginitiationvolumeinnamedcontentcontenttypegenusspeciesescherichiacolinamedcontent
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High Osmolarity Modulates Bacterial Cell Size through Reducing Initiation Volume in <named-content content-type="genus-species">Escherichia coli</named-content>

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