Expression Patterns and Regulation of Non-Coding RNAs during Synthesis of Cellulose in <i>Eucalyptus grandis</i> Hill
Cellulose, an essential structural component in the plant cell wall and a renewable biomass resource, plays a significant role in nature. <i>Eucalyptus’s</i> excellent timber tree species (including <i>Eucalyptus grandis</i> Hill) provide many raw materials for the paper and...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/9d15365786ce40ffa9977ab3fc35f9ee |
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| Sumario: | Cellulose, an essential structural component in the plant cell wall and a renewable biomass resource, plays a significant role in nature. <i>Eucalyptus’s</i> excellent timber tree species (including <i>Eucalyptus grandis</i> Hill) provide many raw materials for the paper and wood industries. The synthesis of cellulose is a very complex process involving multiple genes and regulated by various biological networks. However, research on regulating associated genes and non-coding RNAs during cellulose synthesis in <i>E. grandis</i> remains lacking. In this study, the wood anatomical characteristics and chemical indexes of <i>E. grandis</i> were analyzed by taking three different parts (diameter at breast height (DBH), middle and upper part of the trunk) from the main stem of <i>E. grandis</i> as raw materials. The role of non-coding RNAs (Long non-coding RNA, lncRNA; Micro RNA, miRNA; Circle RNA, circRNA) on regulating candidate genes was presented, and the network map of ceRNA (Competing endogenous RNA) regulation during wood cellulose biosynthesis of <i>E. grandis</i> was constructed. The transcriptome sequencing of nine samples obtained from the trunk of the immature xylem in <i>E. grandis</i> at DBH, middle and upper parts had a 95.81 G clean reading, 57,480 transcripts, 7365 lncRNAs, and 5180 circRNAs. Each sample had 172–306 known miRNAs and 1644–3508 new miRNAs. A total of 190 DE-lncRNAs (Differentially expressed long non-coding RNAs), 174 DE-miRNAs (Differentially expressed micro RNAs), and 270 DE-circRNAs (Differentially expressed circle RNAs) were obtained by comparing transcript expression levels. Four lncRNAs and nine miRNAs were screened out, and the ceRNA regulatory network was constructed. <i>LncRNA1</i> and <i>lncRNA4</i> regulated the genes responsible for cellulose synthesis in <i>E. grandis</i>, which were overexpressed in 84K (<i>Populus Alba</i> × <i>Populus glandulosa</i>) poplar. The cellulose and lignin content in <i>lncRNA4-oe</i> were significantly higher than wild type 84K poplar and <i>lncRNA1-oe</i>. The average plant height, middle and basal part of the stem diameter in <i>lncRNA4-oe</i> were significantly higher than the wild type. However, there was no significant difference between the growth of <i>lncRNA1-oe</i> and the wild type. Further studies are warranted to explore the molecular regulatory mechanism of cellulose biosynthesis in <i>Eucalyptus</i> species. |
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