Interaction of polyethylene glycol with cytochrome c investigated via in vitro and in silico approaches

Abstract One of the significant proteins that have attracted research groups due to virtue of being a potent selective anticancer drug target and property of triggering apoptosis upon release in cytoplasm is cytochrome c (cyt c). The mechanical transformations due to the macromolecular crowding in m...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Zahoor Ahmad Parray, Faizan Ahmad, Mohamed F. Alajmi, Afzal Hussain, Md. Imtaiyaz Hassan, Asimul Islam
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
Materias:
R
Q
Acceso en línea:https://doaj.org/article/9d17ccd8e1d14a8f89cac29f47fd6829
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:9d17ccd8e1d14a8f89cac29f47fd6829
record_format dspace
spelling oai:doaj.org-article:9d17ccd8e1d14a8f89cac29f47fd68292021-12-02T11:39:47ZInteraction of polyethylene glycol with cytochrome c investigated via in vitro and in silico approaches10.1038/s41598-021-85792-42045-2322https://doaj.org/article/9d17ccd8e1d14a8f89cac29f47fd68292021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85792-4https://doaj.org/toc/2045-2322Abstract One of the significant proteins that have attracted research groups due to virtue of being a potent selective anticancer drug target and property of triggering apoptosis upon release in cytoplasm is cytochrome c (cyt c). The mechanical transformations due to the macromolecular crowding in membrane in the mammalian cell are proposed to be useful inductors of changes in volume. It is very interesting to know that mitochondrial function were observed to be improved by polyethylene glycol (PEG) interaction, which in turn inhibits the cyt c (a pro-apoptotic cell death factor). In this work, the effect of polyethylene glycol of molecular weight 4 kilo Dalton (PEG 4 kDa) was investigated to highlight the structural transformations (tertiary and secondary structure) in cyt c using a choice of spectroscopic techniques (including UV–Vis absorption, near-UV, far-UV and Soret circular dichroism and fluorescence spectroscopy), which shows noteworthy shifts in the secondary and tertiary structures at higher concentrations of PEG 4 kDa with small changes in the heme-globular interactions. The size distribution changes of native protein treated with various concentrations of the crowder were observed and analyzed by dynamic light scattering (DLS). The interaction studies of the crowder with the protein was observed and analyzed by FTIR, isothermal titration calorimetry, time resolved fluorescence and molecular docking. The investigations suggested that the structural changes in the protein occurred due to soft interactions of PEG 4 kDa, which usually destabilizes proteins. The experimental evidence in this study proposed that crowding could be another approach to mechanical super-competition and free of certain markers that could aid in the identification and control of various diseases. This study suggests that crowders at specific concentrations, which softly interact with proteins, can be exploited as remedy for various diseases.Zahoor Ahmad ParrayFaizan AhmadMohamed F. AlajmiAfzal HussainMd. Imtaiyaz HassanAsimul IslamNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Zahoor Ahmad Parray
Faizan Ahmad
Mohamed F. Alajmi
Afzal Hussain
Md. Imtaiyaz Hassan
Asimul Islam
Interaction of polyethylene glycol with cytochrome c investigated via in vitro and in silico approaches
description Abstract One of the significant proteins that have attracted research groups due to virtue of being a potent selective anticancer drug target and property of triggering apoptosis upon release in cytoplasm is cytochrome c (cyt c). The mechanical transformations due to the macromolecular crowding in membrane in the mammalian cell are proposed to be useful inductors of changes in volume. It is very interesting to know that mitochondrial function were observed to be improved by polyethylene glycol (PEG) interaction, which in turn inhibits the cyt c (a pro-apoptotic cell death factor). In this work, the effect of polyethylene glycol of molecular weight 4 kilo Dalton (PEG 4 kDa) was investigated to highlight the structural transformations (tertiary and secondary structure) in cyt c using a choice of spectroscopic techniques (including UV–Vis absorption, near-UV, far-UV and Soret circular dichroism and fluorescence spectroscopy), which shows noteworthy shifts in the secondary and tertiary structures at higher concentrations of PEG 4 kDa with small changes in the heme-globular interactions. The size distribution changes of native protein treated with various concentrations of the crowder were observed and analyzed by dynamic light scattering (DLS). The interaction studies of the crowder with the protein was observed and analyzed by FTIR, isothermal titration calorimetry, time resolved fluorescence and molecular docking. The investigations suggested that the structural changes in the protein occurred due to soft interactions of PEG 4 kDa, which usually destabilizes proteins. The experimental evidence in this study proposed that crowding could be another approach to mechanical super-competition and free of certain markers that could aid in the identification and control of various diseases. This study suggests that crowders at specific concentrations, which softly interact with proteins, can be exploited as remedy for various diseases.
format article
author Zahoor Ahmad Parray
Faizan Ahmad
Mohamed F. Alajmi
Afzal Hussain
Md. Imtaiyaz Hassan
Asimul Islam
author_facet Zahoor Ahmad Parray
Faizan Ahmad
Mohamed F. Alajmi
Afzal Hussain
Md. Imtaiyaz Hassan
Asimul Islam
author_sort Zahoor Ahmad Parray
title Interaction of polyethylene glycol with cytochrome c investigated via in vitro and in silico approaches
title_short Interaction of polyethylene glycol with cytochrome c investigated via in vitro and in silico approaches
title_full Interaction of polyethylene glycol with cytochrome c investigated via in vitro and in silico approaches
title_fullStr Interaction of polyethylene glycol with cytochrome c investigated via in vitro and in silico approaches
title_full_unstemmed Interaction of polyethylene glycol with cytochrome c investigated via in vitro and in silico approaches
title_sort interaction of polyethylene glycol with cytochrome c investigated via in vitro and in silico approaches
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/9d17ccd8e1d14a8f89cac29f47fd6829
work_keys_str_mv AT zahoorahmadparray interactionofpolyethyleneglycolwithcytochromecinvestigatedviainvitroandinsilicoapproaches
AT faizanahmad interactionofpolyethyleneglycolwithcytochromecinvestigatedviainvitroandinsilicoapproaches
AT mohamedfalajmi interactionofpolyethyleneglycolwithcytochromecinvestigatedviainvitroandinsilicoapproaches
AT afzalhussain interactionofpolyethyleneglycolwithcytochromecinvestigatedviainvitroandinsilicoapproaches
AT mdimtaiyazhassan interactionofpolyethyleneglycolwithcytochromecinvestigatedviainvitroandinsilicoapproaches
AT asimulislam interactionofpolyethyleneglycolwithcytochromecinvestigatedviainvitroandinsilicoapproaches
_version_ 1718395716074733568