Emergence of bursting activity in connected neuronal sub-populations.

Uniform and modular primary hippocampal cultures from embryonic rats were grown on commercially available micro-electrode arrays to investigate network activity with respect to development and integration of different neuronal populations. Modular networks consisting of two confined active and inter...

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Autores principales:	Marta Bisio, Alessandro Bosca, Valentina Pasquale, Luca Berdondini, Michela Chiappalone
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d19af34f19d4fb79fad0c1cb49fa7f5
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spelling	oai:doaj.org-article:9d19af34f19d4fb79fad0c1cb49fa7f52021-11-25T05:59:37ZEmergence of bursting activity in connected neuronal sub-populations.1932-620310.1371/journal.pone.0107400https://doaj.org/article/9d19af34f19d4fb79fad0c1cb49fa7f52014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0107400https://doaj.org/toc/1932-6203Uniform and modular primary hippocampal cultures from embryonic rats were grown on commercially available micro-electrode arrays to investigate network activity with respect to development and integration of different neuronal populations. Modular networks consisting of two confined active and inter-connected sub-populations of neurons were realized by means of bi-compartmental polydimethylsiloxane structures. Spontaneous activity in both uniform and modular cultures was periodically monitored, from three up to eight weeks after plating. Compared to uniform cultures and despite lower cellular density, modular networks interestingly showed higher firing rates at earlier developmental stages, and network-wide firing and bursting statistics were less variable over time. Although globally less correlated than uniform cultures, modular networks exhibited also higher intra-cluster than inter-cluster correlations, thus demonstrating that segregation and integration of activity coexisted in this simple yet powerful in vitro model. Finally, the peculiar synchronized bursting activity shown by confined modular networks preferentially propagated within one of the two compartments ('dominant'), even in cases of perfect balance of firing rate between the two sub-populations. This dominance was generally maintained during the entire monitored developmental frame, thus suggesting that the implementation of this hierarchy arose from early network development.Marta BisioAlessandro BoscaValentina PasqualeLuca BerdondiniMichela ChiappalonePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e107400 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marta Bisio Alessandro Bosca Valentina Pasquale Luca Berdondini Michela Chiappalone Emergence of bursting activity in connected neuronal sub-populations.
description	Uniform and modular primary hippocampal cultures from embryonic rats were grown on commercially available micro-electrode arrays to investigate network activity with respect to development and integration of different neuronal populations. Modular networks consisting of two confined active and inter-connected sub-populations of neurons were realized by means of bi-compartmental polydimethylsiloxane structures. Spontaneous activity in both uniform and modular cultures was periodically monitored, from three up to eight weeks after plating. Compared to uniform cultures and despite lower cellular density, modular networks interestingly showed higher firing rates at earlier developmental stages, and network-wide firing and bursting statistics were less variable over time. Although globally less correlated than uniform cultures, modular networks exhibited also higher intra-cluster than inter-cluster correlations, thus demonstrating that segregation and integration of activity coexisted in this simple yet powerful in vitro model. Finally, the peculiar synchronized bursting activity shown by confined modular networks preferentially propagated within one of the two compartments ('dominant'), even in cases of perfect balance of firing rate between the two sub-populations. This dominance was generally maintained during the entire monitored developmental frame, thus suggesting that the implementation of this hierarchy arose from early network development.
format	article
author	Marta Bisio Alessandro Bosca Valentina Pasquale Luca Berdondini Michela Chiappalone
author_facet	Marta Bisio Alessandro Bosca Valentina Pasquale Luca Berdondini Michela Chiappalone
author_sort	Marta Bisio
title	Emergence of bursting activity in connected neuronal sub-populations.
title_short	Emergence of bursting activity in connected neuronal sub-populations.
title_full	Emergence of bursting activity in connected neuronal sub-populations.
title_fullStr	Emergence of bursting activity in connected neuronal sub-populations.
title_full_unstemmed	Emergence of bursting activity in connected neuronal sub-populations.
title_sort	emergence of bursting activity in connected neuronal sub-populations.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/9d19af34f19d4fb79fad0c1cb49fa7f5
work_keys_str_mv	AT martabisio emergenceofburstingactivityinconnectedneuronalsubpopulations AT alessandrobosca emergenceofburstingactivityinconnectedneuronalsubpopulations AT valentinapasquale emergenceofburstingactivityinconnectedneuronalsubpopulations AT lucaberdondini emergenceofburstingactivityinconnectedneuronalsubpopulations AT michelachiappalone emergenceofburstingactivityinconnectedneuronalsubpopulations
_version_	1718414296643272704

Emergence of bursting activity in connected neuronal sub-populations.

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