Gene expression profiles during tissue remodeling following bladder outlet obstruction

Abstract Bladder outlet obstruction (BOO) often results in lower urinary tract symptoms (LUTSs) and negatively affects quality of life. Here, we evaluated gene expression patterns in the urinary bladder during tissue remodeling due to BOO. We divided BOO model rats into two groups according to the d...

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Autores principales:	Saya Ito, Takeshi Nomura, Takashi Ueda, Shogo Inui, Yukako Morioka, Hisashi Honjo, Ayako Fukui, Atsuko Fujihara, Fumiya Hongo, Osamu Ukimura
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d1aac88f93c4a0494190c13d14503bf
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spelling	oai:doaj.org-article:9d1aac88f93c4a0494190c13d14503bf2021-12-02T16:07:04ZGene expression profiles during tissue remodeling following bladder outlet obstruction10.1038/s41598-021-92756-12045-2322https://doaj.org/article/9d1aac88f93c4a0494190c13d14503bf2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92756-1https://doaj.org/toc/2045-2322Abstract Bladder outlet obstruction (BOO) often results in lower urinary tract symptoms (LUTSs) and negatively affects quality of life. Here, we evaluated gene expression patterns in the urinary bladder during tissue remodeling due to BOO. We divided BOO model rats into two groups according to the degree of hypertrophy of smooth muscle in the bladder. The strong muscular hypertrophy group, which exhibited markedly increased bladder smooth muscle proportion and HIF1α mRNA levels compared with the control group, was considered a model for the termination of hypertrophy, whereas the mild muscular hypertrophy group was considered a model of the initiation of hypertrophy. Some genes related to urinary function showed different expression patterns between the two groups. Furthermore, we found that several genes, including D-box binding PAR bZIP transcription factor (DBP), were upregulated only in the mild muscular hypertrophy group. DBP expression levels were increased in bladder smooth muscle cells in response to hypoxic stress. DBP associated with enhancer and promoter regions of NOS3 gene locus and upregulated NOS3 gene expression under hypoxic conditions. These findings suggested that the regulatory systems of gene expression were altered during tissue remodeling following BOO. Furthermore, circadian clock components might be involved in control of urinary function via transcriptional gene regulation in response to hypoxic stimuli.Saya ItoTakeshi NomuraTakashi UedaShogo InuiYukako MoriokaHisashi HonjoAyako FukuiAtsuko FujiharaFumiya HongoOsamu UkimuraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Saya Ito Takeshi Nomura Takashi Ueda Shogo Inui Yukako Morioka Hisashi Honjo Ayako Fukui Atsuko Fujihara Fumiya Hongo Osamu Ukimura Gene expression profiles during tissue remodeling following bladder outlet obstruction
description	Abstract Bladder outlet obstruction (BOO) often results in lower urinary tract symptoms (LUTSs) and negatively affects quality of life. Here, we evaluated gene expression patterns in the urinary bladder during tissue remodeling due to BOO. We divided BOO model rats into two groups according to the degree of hypertrophy of smooth muscle in the bladder. The strong muscular hypertrophy group, which exhibited markedly increased bladder smooth muscle proportion and HIF1α mRNA levels compared with the control group, was considered a model for the termination of hypertrophy, whereas the mild muscular hypertrophy group was considered a model of the initiation of hypertrophy. Some genes related to urinary function showed different expression patterns between the two groups. Furthermore, we found that several genes, including D-box binding PAR bZIP transcription factor (DBP), were upregulated only in the mild muscular hypertrophy group. DBP expression levels were increased in bladder smooth muscle cells in response to hypoxic stress. DBP associated with enhancer and promoter regions of NOS3 gene locus and upregulated NOS3 gene expression under hypoxic conditions. These findings suggested that the regulatory systems of gene expression were altered during tissue remodeling following BOO. Furthermore, circadian clock components might be involved in control of urinary function via transcriptional gene regulation in response to hypoxic stimuli.
format	article
author	Saya Ito Takeshi Nomura Takashi Ueda Shogo Inui Yukako Morioka Hisashi Honjo Ayako Fukui Atsuko Fujihara Fumiya Hongo Osamu Ukimura
author_facet	Saya Ito Takeshi Nomura Takashi Ueda Shogo Inui Yukako Morioka Hisashi Honjo Ayako Fukui Atsuko Fujihara Fumiya Hongo Osamu Ukimura
author_sort	Saya Ito
title	Gene expression profiles during tissue remodeling following bladder outlet obstruction
title_short	Gene expression profiles during tissue remodeling following bladder outlet obstruction
title_full	Gene expression profiles during tissue remodeling following bladder outlet obstruction
title_fullStr	Gene expression profiles during tissue remodeling following bladder outlet obstruction
title_full_unstemmed	Gene expression profiles during tissue remodeling following bladder outlet obstruction
title_sort	gene expression profiles during tissue remodeling following bladder outlet obstruction
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9d1aac88f93c4a0494190c13d14503bf
work_keys_str_mv	AT sayaito geneexpressionprofilesduringtissueremodelingfollowingbladderoutletobstruction AT takeshinomura geneexpressionprofilesduringtissueremodelingfollowingbladderoutletobstruction AT takashiueda geneexpressionprofilesduringtissueremodelingfollowingbladderoutletobstruction AT shogoinui geneexpressionprofilesduringtissueremodelingfollowingbladderoutletobstruction AT yukakomorioka geneexpressionprofilesduringtissueremodelingfollowingbladderoutletobstruction AT hisashihonjo geneexpressionprofilesduringtissueremodelingfollowingbladderoutletobstruction AT ayakofukui geneexpressionprofilesduringtissueremodelingfollowingbladderoutletobstruction AT atsukofujihara geneexpressionprofilesduringtissueremodelingfollowingbladderoutletobstruction AT fumiyahongo geneexpressionprofilesduringtissueremodelingfollowingbladderoutletobstruction AT osamuukimura geneexpressionprofilesduringtissueremodelingfollowingbladderoutletobstruction
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Gene expression profiles during tissue remodeling following bladder outlet obstruction

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