Induction of gliotoxin secretion in Aspergillus fumigatus by bacteria-associated molecules.

Aspergillus fumigatus is the most common causative agent of mold diseases in humans, giving rise to life-threatening infections in immunocompromised individuals. One of its secreted metabolites is gliotoxin, a toxic antimicrobial agent. The aim of this study was to determine whether the presence of...

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Autores principales:	K Stefan Svahn, Ulf Göransson, Erja Chryssanthou, Björn Olsen, Jan Sjölin, Adam A Strömstedt
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d1bbcc5e6634814b65ad283f9690221
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spelling	oai:doaj.org-article:9d1bbcc5e6634814b65ad283f96902212021-11-18T08:24:51ZInduction of gliotoxin secretion in Aspergillus fumigatus by bacteria-associated molecules.1932-620310.1371/journal.pone.0093685https://doaj.org/article/9d1bbcc5e6634814b65ad283f96902212014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24705440/?tool=EBIhttps://doaj.org/toc/1932-6203Aspergillus fumigatus is the most common causative agent of mold diseases in humans, giving rise to life-threatening infections in immunocompromised individuals. One of its secreted metabolites is gliotoxin, a toxic antimicrobial agent. The aim of this study was to determine whether the presence of pathogen-associated molecular patterns in broth cultures of A. fumigatus could induce gliotoxin production. Gliotoxin levels were analyzed by ultra-performance liquid chromatography and mass spectrometry. The presence of a bacteria-derived lipopolysaccharide, peptidoglycan, or lipoteichoic acid in the growth media at a concentration of 5 μg/ml increased the gliotoxin concentration in the media by 37%, 65%, and 35%, respectively. The findings reveal a correlation between the concentrations of pathogen-associated molecular patterns and gliotoxin secretion. This shows that there is a yet uncharacterized detection system for such compounds within fungi. Inducing secondary metabolite production by such means in fungi is potentially relevant for drug discovery research. Our results also give a possible explanation for the increased virulence of A. fumigatus during bacterial co-infection, one that is important for the transition from colonization to invasiveness in this pulmonary disease.K Stefan SvahnUlf GöranssonErja ChryssanthouBjörn OlsenJan SjölinAdam A StrömstedtPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e93685 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q K Stefan Svahn Ulf Göransson Erja Chryssanthou Björn Olsen Jan Sjölin Adam A Strömstedt Induction of gliotoxin secretion in Aspergillus fumigatus by bacteria-associated molecules.
description	Aspergillus fumigatus is the most common causative agent of mold diseases in humans, giving rise to life-threatening infections in immunocompromised individuals. One of its secreted metabolites is gliotoxin, a toxic antimicrobial agent. The aim of this study was to determine whether the presence of pathogen-associated molecular patterns in broth cultures of A. fumigatus could induce gliotoxin production. Gliotoxin levels were analyzed by ultra-performance liquid chromatography and mass spectrometry. The presence of a bacteria-derived lipopolysaccharide, peptidoglycan, or lipoteichoic acid in the growth media at a concentration of 5 μg/ml increased the gliotoxin concentration in the media by 37%, 65%, and 35%, respectively. The findings reveal a correlation between the concentrations of pathogen-associated molecular patterns and gliotoxin secretion. This shows that there is a yet uncharacterized detection system for such compounds within fungi. Inducing secondary metabolite production by such means in fungi is potentially relevant for drug discovery research. Our results also give a possible explanation for the increased virulence of A. fumigatus during bacterial co-infection, one that is important for the transition from colonization to invasiveness in this pulmonary disease.
format	article
author	K Stefan Svahn Ulf Göransson Erja Chryssanthou Björn Olsen Jan Sjölin Adam A Strömstedt
author_facet	K Stefan Svahn Ulf Göransson Erja Chryssanthou Björn Olsen Jan Sjölin Adam A Strömstedt
author_sort	K Stefan Svahn
title	Induction of gliotoxin secretion in Aspergillus fumigatus by bacteria-associated molecules.
title_short	Induction of gliotoxin secretion in Aspergillus fumigatus by bacteria-associated molecules.
title_full	Induction of gliotoxin secretion in Aspergillus fumigatus by bacteria-associated molecules.
title_fullStr	Induction of gliotoxin secretion in Aspergillus fumigatus by bacteria-associated molecules.
title_full_unstemmed	Induction of gliotoxin secretion in Aspergillus fumigatus by bacteria-associated molecules.
title_sort	induction of gliotoxin secretion in aspergillus fumigatus by bacteria-associated molecules.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/9d1bbcc5e6634814b65ad283f9690221
work_keys_str_mv	AT kstefansvahn inductionofgliotoxinsecretioninaspergillusfumigatusbybacteriaassociatedmolecules AT ulfgoransson inductionofgliotoxinsecretioninaspergillusfumigatusbybacteriaassociatedmolecules AT erjachryssanthou inductionofgliotoxinsecretioninaspergillusfumigatusbybacteriaassociatedmolecules AT bjornolsen inductionofgliotoxinsecretioninaspergillusfumigatusbybacteriaassociatedmolecules AT jansjolin inductionofgliotoxinsecretioninaspergillusfumigatusbybacteriaassociatedmolecules AT adamastromstedt inductionofgliotoxinsecretioninaspergillusfumigatusbybacteriaassociatedmolecules
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Induction of gliotoxin secretion in Aspergillus fumigatus by bacteria-associated molecules.

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