Effects of urethane and isoflurane on the sensory evoked response and local blood flow in the early postnatal rat somatosensory cortex

Abstract Functional studies in the central nervous system are often conducted using anesthesia. While the dose-dependent effects of anesthesia on neuronal activity have been extensively characterized in adults, little is known about the effects of anesthesia on cortical activity and cerebral blood f...

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Autores principales:	Viktoria Shumkova, Violetta Sitdikova, Ildar Rechapov, Alexey Leukhin, Marat Minlebaev
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d250de676e1495e9a4e3074c01950ac
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spelling	oai:doaj.org-article:9d250de676e1495e9a4e3074c01950ac2021-12-02T14:49:25ZEffects of urethane and isoflurane on the sensory evoked response and local blood flow in the early postnatal rat somatosensory cortex10.1038/s41598-021-88461-82045-2322https://doaj.org/article/9d250de676e1495e9a4e3074c01950ac2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88461-8https://doaj.org/toc/2045-2322Abstract Functional studies in the central nervous system are often conducted using anesthesia. While the dose-dependent effects of anesthesia on neuronal activity have been extensively characterized in adults, little is known about the effects of anesthesia on cortical activity and cerebral blood flow in the immature central nervous system. Substitution of electrophysiological recordings with the less-invasive technique of optical intrinsic signal imaging (OIS) in vivo allowed simultaneous recordings of sensory-evoked functional response and local blood flow changes in the neonatal rat barrel cortex. Using OIS we characterize the effects of two widely used anesthetics—urethane and isoflurane. We found that both anesthetics suppressed the sensory-evoked optical intrinsic signal in a dose-dependent manner. Dependence of the cortical response suppression matched the exponential decay model. At experimental levels of anesthesia, urethane affected the evoked cortical response less than isoflurane, which is in agreement with the results of electrophysiological recordings demonstrated by other authors. Changes in oxygenation and local blood flow also showed negative correlation with both anesthetics. The high similarity in immature patterns of activity recorded in different regions of the developing cortex suggested similar principles of development regardless of the cortical region. Therefore the indicated results should be taken into account during functional explorations in the entire developing cortex. Our results also point to urethane as the anesthetic of choice in non-survival experimental recordings in the developing brain as it produces less prominent impairment of cortical neuronal activity in neonatal animals.Viktoria ShumkovaVioletta SitdikovaIldar RechapovAlexey LeukhinMarat MinlebaevNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Viktoria Shumkova Violetta Sitdikova Ildar Rechapov Alexey Leukhin Marat Minlebaev Effects of urethane and isoflurane on the sensory evoked response and local blood flow in the early postnatal rat somatosensory cortex
description	Abstract Functional studies in the central nervous system are often conducted using anesthesia. While the dose-dependent effects of anesthesia on neuronal activity have been extensively characterized in adults, little is known about the effects of anesthesia on cortical activity and cerebral blood flow in the immature central nervous system. Substitution of electrophysiological recordings with the less-invasive technique of optical intrinsic signal imaging (OIS) in vivo allowed simultaneous recordings of sensory-evoked functional response and local blood flow changes in the neonatal rat barrel cortex. Using OIS we characterize the effects of two widely used anesthetics—urethane and isoflurane. We found that both anesthetics suppressed the sensory-evoked optical intrinsic signal in a dose-dependent manner. Dependence of the cortical response suppression matched the exponential decay model. At experimental levels of anesthesia, urethane affected the evoked cortical response less than isoflurane, which is in agreement with the results of electrophysiological recordings demonstrated by other authors. Changes in oxygenation and local blood flow also showed negative correlation with both anesthetics. The high similarity in immature patterns of activity recorded in different regions of the developing cortex suggested similar principles of development regardless of the cortical region. Therefore the indicated results should be taken into account during functional explorations in the entire developing cortex. Our results also point to urethane as the anesthetic of choice in non-survival experimental recordings in the developing brain as it produces less prominent impairment of cortical neuronal activity in neonatal animals.
format	article
author	Viktoria Shumkova Violetta Sitdikova Ildar Rechapov Alexey Leukhin Marat Minlebaev
author_facet	Viktoria Shumkova Violetta Sitdikova Ildar Rechapov Alexey Leukhin Marat Minlebaev
author_sort	Viktoria Shumkova
title	Effects of urethane and isoflurane on the sensory evoked response and local blood flow in the early postnatal rat somatosensory cortex
title_short	Effects of urethane and isoflurane on the sensory evoked response and local blood flow in the early postnatal rat somatosensory cortex
title_full	Effects of urethane and isoflurane on the sensory evoked response and local blood flow in the early postnatal rat somatosensory cortex
title_fullStr	Effects of urethane and isoflurane on the sensory evoked response and local blood flow in the early postnatal rat somatosensory cortex
title_full_unstemmed	Effects of urethane and isoflurane on the sensory evoked response and local blood flow in the early postnatal rat somatosensory cortex
title_sort	effects of urethane and isoflurane on the sensory evoked response and local blood flow in the early postnatal rat somatosensory cortex
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9d250de676e1495e9a4e3074c01950ac
work_keys_str_mv	AT viktoriashumkova effectsofurethaneandisofluraneonthesensoryevokedresponseandlocalbloodflowintheearlypostnatalratsomatosensorycortex AT violettasitdikova effectsofurethaneandisofluraneonthesensoryevokedresponseandlocalbloodflowintheearlypostnatalratsomatosensorycortex AT ildarrechapov effectsofurethaneandisofluraneonthesensoryevokedresponseandlocalbloodflowintheearlypostnatalratsomatosensorycortex AT alexeyleukhin effectsofurethaneandisofluraneonthesensoryevokedresponseandlocalbloodflowintheearlypostnatalratsomatosensorycortex AT maratminlebaev effectsofurethaneandisofluraneonthesensoryevokedresponseandlocalbloodflowintheearlypostnatalratsomatosensorycortex
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Effects of urethane and isoflurane on the sensory evoked response and local blood flow in the early postnatal rat somatosensory cortex

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