The long coiled-coil protein NECC2 is associated to caveolae and modulates NGF/TrkA signaling in PC12 cells [corrected].

TrkA-mediated NGF signaling in PC12 cells has been shown to be compartimentalized in specialized microdomains of the plasma membrane, the caveolae, which are organized by scaffold proteins including the member of the caveolin family of proteins, caveolin-1. Here, we characterize the intracellular di...

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Autores principales:	Alberto Díaz-Ruiz, Yoana Rabanal-Ruiz, Andrés Trávez, Francisco Gracia-Navarro, David Cruz-García, Maité Montero-Hadjadje, Youssef Anouar, Stéphane Gasman, Nicolas Vitale, Rafael Vázquez-Martínez, María M Malagón
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d29af437963483fa7b62a62ff4c1496
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spelling	oai:doaj.org-article:9d29af437963483fa7b62a62ff4c14962021-11-18T08:56:30ZThe long coiled-coil protein NECC2 is associated to caveolae and modulates NGF/TrkA signaling in PC12 cells [corrected].1932-620310.1371/journal.pone.0073668https://doaj.org/article/9d29af437963483fa7b62a62ff4c14962013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24040018/?tool=EBIhttps://doaj.org/toc/1932-6203TrkA-mediated NGF signaling in PC12 cells has been shown to be compartimentalized in specialized microdomains of the plasma membrane, the caveolae, which are organized by scaffold proteins including the member of the caveolin family of proteins, caveolin-1. Here, we characterize the intracellular distribution as well as the biochemical and functional properties of the neuroendocrine long coiled-coil protein 2 (NECC2), a novel long coiled-coil protein selectively expressed in neuroendocrine tissues that contains a predicted caveolin-binding domain and displays structural characteristics of a scaffolding factor. NECC2 distributes in caveolae, wherein it colocalizes with the TrkA receptor, and behaves as a caveolae-associated protein in neuroendocrine PC12 cells. In addition, stimulation of PC12 cells with nerve growth factor (NGF) increased the expression and regulated the distribution of NECC2. Interestingly, knockdown as well as overexpression of NECC2 resulted in a reduction of NGF-induced phosphorylation of the TrkA downstream effector extracellular signal-regulated kinases 1 and 2 (ERK1/ERK2) but not of Akt. Altogether, our results identify NECC2 as a novel component of caveolae in PC12 cells and support the contribution of this protein in the maintenance of TrkA-mediated NGF signaling.Alberto Díaz-RuizYoana Rabanal-RuizAndrés TrávezFrancisco Gracia-NavarroDavid Cruz-GarcíaMaité Montero-HadjadjeYoussef AnouarStéphane GasmanNicolas VitaleRafael Vázquez-MartínezMaría M MalagónPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e73668 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alberto Díaz-Ruiz Yoana Rabanal-Ruiz Andrés Trávez Francisco Gracia-Navarro David Cruz-García Maité Montero-Hadjadje Youssef Anouar Stéphane Gasman Nicolas Vitale Rafael Vázquez-Martínez María M Malagón The long coiled-coil protein NECC2 is associated to caveolae and modulates NGF/TrkA signaling in PC12 cells [corrected].
description	TrkA-mediated NGF signaling in PC12 cells has been shown to be compartimentalized in specialized microdomains of the plasma membrane, the caveolae, which are organized by scaffold proteins including the member of the caveolin family of proteins, caveolin-1. Here, we characterize the intracellular distribution as well as the biochemical and functional properties of the neuroendocrine long coiled-coil protein 2 (NECC2), a novel long coiled-coil protein selectively expressed in neuroendocrine tissues that contains a predicted caveolin-binding domain and displays structural characteristics of a scaffolding factor. NECC2 distributes in caveolae, wherein it colocalizes with the TrkA receptor, and behaves as a caveolae-associated protein in neuroendocrine PC12 cells. In addition, stimulation of PC12 cells with nerve growth factor (NGF) increased the expression and regulated the distribution of NECC2. Interestingly, knockdown as well as overexpression of NECC2 resulted in a reduction of NGF-induced phosphorylation of the TrkA downstream effector extracellular signal-regulated kinases 1 and 2 (ERK1/ERK2) but not of Akt. Altogether, our results identify NECC2 as a novel component of caveolae in PC12 cells and support the contribution of this protein in the maintenance of TrkA-mediated NGF signaling.
format	article
author	Alberto Díaz-Ruiz Yoana Rabanal-Ruiz Andrés Trávez Francisco Gracia-Navarro David Cruz-García Maité Montero-Hadjadje Youssef Anouar Stéphane Gasman Nicolas Vitale Rafael Vázquez-Martínez María M Malagón
author_facet	Alberto Díaz-Ruiz Yoana Rabanal-Ruiz Andrés Trávez Francisco Gracia-Navarro David Cruz-García Maité Montero-Hadjadje Youssef Anouar Stéphane Gasman Nicolas Vitale Rafael Vázquez-Martínez María M Malagón
author_sort	Alberto Díaz-Ruiz
title	The long coiled-coil protein NECC2 is associated to caveolae and modulates NGF/TrkA signaling in PC12 cells [corrected].
title_short	The long coiled-coil protein NECC2 is associated to caveolae and modulates NGF/TrkA signaling in PC12 cells [corrected].
title_full	The long coiled-coil protein NECC2 is associated to caveolae and modulates NGF/TrkA signaling in PC12 cells [corrected].
title_fullStr	The long coiled-coil protein NECC2 is associated to caveolae and modulates NGF/TrkA signaling in PC12 cells [corrected].
title_full_unstemmed	The long coiled-coil protein NECC2 is associated to caveolae and modulates NGF/TrkA signaling in PC12 cells [corrected].
title_sort	long coiled-coil protein necc2 is associated to caveolae and modulates ngf/trka signaling in pc12 cells [corrected].
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/9d29af437963483fa7b62a62ff4c1496
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The long coiled-coil protein NECC2 is associated to caveolae and modulates NGF/TrkA signaling in PC12 cells [corrected].

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