Drosophila oocyte proteome composition covaries with female mating status

Abstract Oocyte composition can directly influence offspring fitness, particularly in oviparous species such as most insects, where it is the primary form of parental investment. Oocyte production is also energetically costly, dependent on female condition and responsive to external cues. Here, we i...

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Autores principales:	Caitlin E. McDonough-Goldstein, Scott Pitnick, Steve Dorus
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d2b801343104da29640fbe42487d16a
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spelling	oai:doaj.org-article:9d2b801343104da29640fbe42487d16a2021-12-02T10:44:16ZDrosophila oocyte proteome composition covaries with female mating status10.1038/s41598-021-82801-42045-2322https://doaj.org/article/9d2b801343104da29640fbe42487d16a2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82801-4https://doaj.org/toc/2045-2322Abstract Oocyte composition can directly influence offspring fitness, particularly in oviparous species such as most insects, where it is the primary form of parental investment. Oocyte production is also energetically costly, dependent on female condition and responsive to external cues. Here, we investigated whether mating influences mature oocyte composition in Drosophila melanogaster using a quantitative proteomic approach. Our analyses robustly identified 4,485 oocyte proteins and revealed that stage-14 oocytes from mated females differed significantly in protein composition relative to oocytes from unmated females. Proteins forming a highly interconnected network enriched for translational machinery and transmembrane proteins were increased in oocytes from mated females, including calcium binding and transport proteins. This mating-induced modulation of oocyte maturation was also significantly associated with proteome changes that are known to be triggered by egg activation. We propose that these compositional changes are likely to have fitness consequences and adaptive implications given the importance of oocyte protein composition, rather than active gene expression, to the maternal-to-zygotic transition and early embryogenesis.Caitlin E. McDonough-GoldsteinScott PitnickSteve DorusNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Caitlin E. McDonough-Goldstein Scott Pitnick Steve Dorus Drosophila oocyte proteome composition covaries with female mating status
description	Abstract Oocyte composition can directly influence offspring fitness, particularly in oviparous species such as most insects, where it is the primary form of parental investment. Oocyte production is also energetically costly, dependent on female condition and responsive to external cues. Here, we investigated whether mating influences mature oocyte composition in Drosophila melanogaster using a quantitative proteomic approach. Our analyses robustly identified 4,485 oocyte proteins and revealed that stage-14 oocytes from mated females differed significantly in protein composition relative to oocytes from unmated females. Proteins forming a highly interconnected network enriched for translational machinery and transmembrane proteins were increased in oocytes from mated females, including calcium binding and transport proteins. This mating-induced modulation of oocyte maturation was also significantly associated with proteome changes that are known to be triggered by egg activation. We propose that these compositional changes are likely to have fitness consequences and adaptive implications given the importance of oocyte protein composition, rather than active gene expression, to the maternal-to-zygotic transition and early embryogenesis.
format	article
author	Caitlin E. McDonough-Goldstein Scott Pitnick Steve Dorus
author_facet	Caitlin E. McDonough-Goldstein Scott Pitnick Steve Dorus
author_sort	Caitlin E. McDonough-Goldstein
title	Drosophila oocyte proteome composition covaries with female mating status
title_short	Drosophila oocyte proteome composition covaries with female mating status
title_full	Drosophila oocyte proteome composition covaries with female mating status
title_fullStr	Drosophila oocyte proteome composition covaries with female mating status
title_full_unstemmed	Drosophila oocyte proteome composition covaries with female mating status
title_sort	drosophila oocyte proteome composition covaries with female mating status
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9d2b801343104da29640fbe42487d16a
work_keys_str_mv	AT caitlinemcdonoughgoldstein drosophilaoocyteproteomecompositioncovarieswithfemalematingstatus AT scottpitnick drosophilaoocyteproteomecompositioncovarieswithfemalematingstatus AT stevedorus drosophilaoocyteproteomecompositioncovarieswithfemalematingstatus
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Drosophila oocyte proteome composition covaries with female mating status

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