Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion

Abstract Genome editing has emerged as a technology with a potential to revolutionize plant breeding. In this study, we report on generating, in less than ten months, Tomelo, a non-transgenic tomato variety resistant to the powdery mildew fungal pathogen using the CRISPR/Cas9 technology. We used who...

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Autores principales:	Vladimir Nekrasov, Congmao Wang, Joe Win, Christa Lanz, Detlef Weigel, Sophien Kamoun
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d30a8e741474e99ad15e91a68411d31
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spelling	oai:doaj.org-article:9d30a8e741474e99ad15e91a68411d312021-12-02T15:06:19ZRapid generation of a transgene-free powdery mildew resistant tomato by genome deletion10.1038/s41598-017-00578-x2045-2322https://doaj.org/article/9d30a8e741474e99ad15e91a68411d312017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00578-xhttps://doaj.org/toc/2045-2322Abstract Genome editing has emerged as a technology with a potential to revolutionize plant breeding. In this study, we report on generating, in less than ten months, Tomelo, a non-transgenic tomato variety resistant to the powdery mildew fungal pathogen using the CRISPR/Cas9 technology. We used whole-genome sequencing to show that Tomelo does not carry any foreign DNA sequences but only carries a deletion that is indistinguishable from naturally occurring mutations. We also present evidence for CRISPR/Cas9 being a highly precise tool, as we did not detect off-target mutations in Tomelo. Using our pipeline, mutations can be readily introduced into elite or locally adapted tomato varieties in less than a year with relatively minimal effort and investment.Vladimir NekrasovCongmao WangJoe WinChrista LanzDetlef WeigelSophien KamounNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-6 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vladimir Nekrasov Congmao Wang Joe Win Christa Lanz Detlef Weigel Sophien Kamoun Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion
description	Abstract Genome editing has emerged as a technology with a potential to revolutionize plant breeding. In this study, we report on generating, in less than ten months, Tomelo, a non-transgenic tomato variety resistant to the powdery mildew fungal pathogen using the CRISPR/Cas9 technology. We used whole-genome sequencing to show that Tomelo does not carry any foreign DNA sequences but only carries a deletion that is indistinguishable from naturally occurring mutations. We also present evidence for CRISPR/Cas9 being a highly precise tool, as we did not detect off-target mutations in Tomelo. Using our pipeline, mutations can be readily introduced into elite or locally adapted tomato varieties in less than a year with relatively minimal effort and investment.
format	article
author	Vladimir Nekrasov Congmao Wang Joe Win Christa Lanz Detlef Weigel Sophien Kamoun
author_facet	Vladimir Nekrasov Congmao Wang Joe Win Christa Lanz Detlef Weigel Sophien Kamoun
author_sort	Vladimir Nekrasov
title	Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion
title_short	Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion
title_full	Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion
title_fullStr	Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion
title_full_unstemmed	Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion
title_sort	rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9d30a8e741474e99ad15e91a68411d31
work_keys_str_mv	AT vladimirnekrasov rapidgenerationofatransgenefreepowderymildewresistanttomatobygenomedeletion AT congmaowang rapidgenerationofatransgenefreepowderymildewresistanttomatobygenomedeletion AT joewin rapidgenerationofatransgenefreepowderymildewresistanttomatobygenomedeletion AT christalanz rapidgenerationofatransgenefreepowderymildewresistanttomatobygenomedeletion AT detlefweigel rapidgenerationofatransgenefreepowderymildewresistanttomatobygenomedeletion AT sophienkamoun rapidgenerationofatransgenefreepowderymildewresistanttomatobygenomedeletion
_version_	1718388531560185856

Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion

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