Tissue-specific epigenetic modifications in root apical meristem cells of Hordeum vulgare.

Epigenetic modifications of chromatin structure are essential for many biological processes, including growth and reproduction. Patterns of DNA and histone modifications have recently been widely studied in many plant species, although there is virtually no data on the spatial and temporal distribut...

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Autores principales:	Agnieszka J Braszewska-Zalewska, Elzbieta A Wolny, Lukasz Smialek, Robert Hasterok
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d328573e4844e4b8e83c662990f9135
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spelling	oai:doaj.org-article:9d328573e4844e4b8e83c662990f91352021-11-18T09:01:52ZTissue-specific epigenetic modifications in root apical meristem cells of Hordeum vulgare.1932-620310.1371/journal.pone.0069204https://doaj.org/article/9d328573e4844e4b8e83c662990f91352013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23935955/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Epigenetic modifications of chromatin structure are essential for many biological processes, including growth and reproduction. Patterns of DNA and histone modifications have recently been widely studied in many plant species, although there is virtually no data on the spatial and temporal distribution of epigenetic markers during plant development. Accordingly, we have used immunostaining techniques to investigate epigenetic modifications in the root apical meristem of Hordeum vulgare. Histone H4 acetylation (H4K5ac), histone H3 dimethylation (H3K4me2, H3K9me2) and DNA methylation (5mC) patterns were established for various root meristem tissues. Distinct levels of those modifications were visualised in the root cap, epidermis, cortex and vascular tissues. The lateral root cap cells seem to display the highest level of H3K9me2 and 5mC. In the epidermis, the highest level of 5mC and H3K9me2 was detected in the nuclei from the boundary of the proximal meristem and the elongation zone, while the vascular tissues were characterized by the highest level of H4K5ac. Some of the modified histones were also detectable in the cytoplasm in a highly tissue-specific manner. Immunolocalisation of epigenetic modifications of chromatin carried out in this way, on longitudinal or transverse sections, provides a unique topographic context within the organ, and will provide some answers to the significant biological question of tissue differentiation processes during root development in a monocotyledon plant species.Agnieszka J Braszewska-ZalewskaElzbieta A WolnyLukasz SmialekRobert HasterokPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e69204 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Agnieszka J Braszewska-Zalewska Elzbieta A Wolny Lukasz Smialek Robert Hasterok Tissue-specific epigenetic modifications in root apical meristem cells of Hordeum vulgare.
description	Epigenetic modifications of chromatin structure are essential for many biological processes, including growth and reproduction. Patterns of DNA and histone modifications have recently been widely studied in many plant species, although there is virtually no data on the spatial and temporal distribution of epigenetic markers during plant development. Accordingly, we have used immunostaining techniques to investigate epigenetic modifications in the root apical meristem of Hordeum vulgare. Histone H4 acetylation (H4K5ac), histone H3 dimethylation (H3K4me2, H3K9me2) and DNA methylation (5mC) patterns were established for various root meristem tissues. Distinct levels of those modifications were visualised in the root cap, epidermis, cortex and vascular tissues. The lateral root cap cells seem to display the highest level of H3K9me2 and 5mC. In the epidermis, the highest level of 5mC and H3K9me2 was detected in the nuclei from the boundary of the proximal meristem and the elongation zone, while the vascular tissues were characterized by the highest level of H4K5ac. Some of the modified histones were also detectable in the cytoplasm in a highly tissue-specific manner. Immunolocalisation of epigenetic modifications of chromatin carried out in this way, on longitudinal or transverse sections, provides a unique topographic context within the organ, and will provide some answers to the significant biological question of tissue differentiation processes during root development in a monocotyledon plant species.
format	article
author	Agnieszka J Braszewska-Zalewska Elzbieta A Wolny Lukasz Smialek Robert Hasterok
author_facet	Agnieszka J Braszewska-Zalewska Elzbieta A Wolny Lukasz Smialek Robert Hasterok
author_sort	Agnieszka J Braszewska-Zalewska
title	Tissue-specific epigenetic modifications in root apical meristem cells of Hordeum vulgare.
title_short	Tissue-specific epigenetic modifications in root apical meristem cells of Hordeum vulgare.
title_full	Tissue-specific epigenetic modifications in root apical meristem cells of Hordeum vulgare.
title_fullStr	Tissue-specific epigenetic modifications in root apical meristem cells of Hordeum vulgare.
title_full_unstemmed	Tissue-specific epigenetic modifications in root apical meristem cells of Hordeum vulgare.
title_sort	tissue-specific epigenetic modifications in root apical meristem cells of hordeum vulgare.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/9d328573e4844e4b8e83c662990f9135
work_keys_str_mv	AT agnieszkajbraszewskazalewska tissuespecificepigeneticmodificationsinrootapicalmeristemcellsofhordeumvulgare AT elzbietaawolny tissuespecificepigeneticmodificationsinrootapicalmeristemcellsofhordeumvulgare AT lukaszsmialek tissuespecificepigeneticmodificationsinrootapicalmeristemcellsofhordeumvulgare AT roberthasterok tissuespecificepigeneticmodificationsinrootapicalmeristemcellsofhordeumvulgare
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Tissue-specific epigenetic modifications in root apical meristem cells of Hordeum vulgare.

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