DNA Isolation of Chicken Feathers from the Base of the Young Feathers, the Base of the Old Feathers, and the Tip of the Feathers

Background: The goal was to provide information on the use of DNA templates in chicken samples so that the molecular research sampling process may employ feathers instead of hurting the test animals. Method: The sample used consisted of 10 Bangkok chickens which were sampled for young feathers and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autor principal:	Alfi Sophian
Formato:	article
Lenguaje:	EN ID
Publicado:	Department of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah 2021
Materias:	base of feathers chicken end of feathers feather Education L Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9d3e3946494648deb3af3e46a2746fd8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9d3e3946494648deb3af3e46a2746fd8
record_format	dspace
spelling	oai:doaj.org-article:9d3e3946494648deb3af3e46a2746fd82021-11-22T13:46:03ZDNA Isolation of Chicken Feathers from the Base of the Young Feathers, the Base of the Old Feathers, and the Tip of the Feathers2614-154X2614-155810.22236/j.bes/526211https://doaj.org/article/9d3e3946494648deb3af3e46a2746fd82021-08-01T00:00:00Zhttps://journal.uhamka.ac.id/index.php/bioeduscience/article/view/6211https://doaj.org/toc/2614-154Xhttps://doaj.org/toc/2614-1558Background: The goal was to provide information on the use of DNA templates in chicken samples so that the molecular research sampling process may employ feathers instead of hurting the test animals. Method: The sample used consisted of 10 Bangkok chickens which were sampled for young feathers and old feathers and the tips of the feathers. Quantitative techniques by comparing the results of DNA isolation which were analyzed using a nano photometer and then confirmed using real-time PCR with the SYBR green method. Result: The analysis of purity and concentration showed that at the base of young chicken feathers, the average value of purity was at 1,790, with an average value of the concentration of 4,210. At the base of the old feather, the average value of purity was 0.638, with an average concentration value that was not detected. Likewise, at the tip of the feather, the average purity value is 0.894 and the concentration value is not detected. Confirmation tests performed on all samples using the real-time PCR melt curve method showed that all samples were detected with a Tm value of 78.5 for young feathers, 78.5 for old feathers, 79.0 for positive controls and 78.7 for positive controls, while negative controls were not detected. Conclusion: DNA isolation can be carried out at the base of the young feathers, the base of the old feathers and the tips of the feathers.Alfi SophianDepartment of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyaharticlebase of featherschickenend of feathersfeatherEducationLBiology (General)QH301-705.5ENIDBioeduscience, Vol 5, Iss 2, Pp 104-108 (2021)
institution	DOAJ
collection	DOAJ
language	EN ID
topic	base of feathers chicken end of feathers feather Education L Biology (General) QH301-705.5
spellingShingle	base of feathers chicken end of feathers feather Education L Biology (General) QH301-705.5 Alfi Sophian DNA Isolation of Chicken Feathers from the Base of the Young Feathers, the Base of the Old Feathers, and the Tip of the Feathers
description	Background: The goal was to provide information on the use of DNA templates in chicken samples so that the molecular research sampling process may employ feathers instead of hurting the test animals. Method: The sample used consisted of 10 Bangkok chickens which were sampled for young feathers and old feathers and the tips of the feathers. Quantitative techniques by comparing the results of DNA isolation which were analyzed using a nano photometer and then confirmed using real-time PCR with the SYBR green method. Result: The analysis of purity and concentration showed that at the base of young chicken feathers, the average value of purity was at 1,790, with an average value of the concentration of 4,210. At the base of the old feather, the average value of purity was 0.638, with an average concentration value that was not detected. Likewise, at the tip of the feather, the average purity value is 0.894 and the concentration value is not detected. Confirmation tests performed on all samples using the real-time PCR melt curve method showed that all samples were detected with a Tm value of 78.5 for young feathers, 78.5 for old feathers, 79.0 for positive controls and 78.7 for positive controls, while negative controls were not detected. Conclusion: DNA isolation can be carried out at the base of the young feathers, the base of the old feathers and the tips of the feathers.
format	article
author	Alfi Sophian
author_facet	Alfi Sophian
author_sort	Alfi Sophian
title	DNA Isolation of Chicken Feathers from the Base of the Young Feathers, the Base of the Old Feathers, and the Tip of the Feathers
title_short	DNA Isolation of Chicken Feathers from the Base of the Young Feathers, the Base of the Old Feathers, and the Tip of the Feathers
title_full	DNA Isolation of Chicken Feathers from the Base of the Young Feathers, the Base of the Old Feathers, and the Tip of the Feathers
title_fullStr	DNA Isolation of Chicken Feathers from the Base of the Young Feathers, the Base of the Old Feathers, and the Tip of the Feathers
title_full_unstemmed	DNA Isolation of Chicken Feathers from the Base of the Young Feathers, the Base of the Old Feathers, and the Tip of the Feathers
title_sort	dna isolation of chicken feathers from the base of the young feathers, the base of the old feathers, and the tip of the feathers
publisher	Department of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah
publishDate	2021
url	https://doaj.org/article/9d3e3946494648deb3af3e46a2746fd8
work_keys_str_mv	AT alfisophian dnaisolationofchickenfeathersfromthebaseoftheyoungfeathersthebaseoftheoldfeathersandthetipofthefeathers
_version_	1718417569664204800

DNA Isolation of Chicken Feathers from the Base of the Young Feathers, the Base of the Old Feathers, and the Tip of the Feathers

Ejemplares similares