Leucine supplementation improves skeletal muscle regeneration after cryolesion in rats.

This study was undertaken in order to provide further insight into the role of leucine supplementation in the skeletal muscle regeneration process, focusing on myofiber size and strength recovery. Young (2-month-old) rats were subjected or not to leucine supplementation (1.35 g/kg per day) started 3...

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Autores principales:	Marcelo G Pereira, Igor L Baptista, Eduardo O C Carlassara, Anselmo S Moriscot, Marcelo S Aoki, Elen H Miyabara
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9d3e9036147f40b4b71d154cc581e654
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spelling	oai:doaj.org-article:9d3e9036147f40b4b71d154cc581e6542021-11-18T08:38:24ZLeucine supplementation improves skeletal muscle regeneration after cryolesion in rats.1932-620310.1371/journal.pone.0085283https://doaj.org/article/9d3e9036147f40b4b71d154cc581e6542014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24416379/?tool=EBIhttps://doaj.org/toc/1932-6203This study was undertaken in order to provide further insight into the role of leucine supplementation in the skeletal muscle regeneration process, focusing on myofiber size and strength recovery. Young (2-month-old) rats were subjected or not to leucine supplementation (1.35 g/kg per day) started 3 days prior to cryolesion. Then, soleus muscles were cryolesioned and continued receiving leucine supplementation until 1, 3 and 10 days later. Soleus muscles from leucine-supplemented animals displayed an increase in myofiber size and a reduction in collagen type III expression on post-cryolesion day 10. Leucine was also effective in reducing FOXO3a activation and ubiquitinated protein accumulation in muscles at post-cryolesion days 3 and 10. In addition, leucine supplementation minimized the cryolesion-induced decrease in tetanic strength and increase in fatigue in regenerating muscles at post-cryolesion day 10. These beneficial effects of leucine were not accompanied by activation of any elements of the phosphoinositide 3-kinase/Akt/mechanistic target of rapamycin signalling pathway in the regenerating muscles. Our results show that leucine improves myofiber size gain and strength recovery in regenerating soleus muscles through attenuation of protein ubiquitination. In addition, leucine might have therapeutic effects for muscle recovery following injury and in some muscle diseases.Marcelo G PereiraIgor L BaptistaEduardo O C CarlassaraAnselmo S MoriscotMarcelo S AokiElen H MiyabaraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e85283 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marcelo G Pereira Igor L Baptista Eduardo O C Carlassara Anselmo S Moriscot Marcelo S Aoki Elen H Miyabara Leucine supplementation improves skeletal muscle regeneration after cryolesion in rats.
description	This study was undertaken in order to provide further insight into the role of leucine supplementation in the skeletal muscle regeneration process, focusing on myofiber size and strength recovery. Young (2-month-old) rats were subjected or not to leucine supplementation (1.35 g/kg per day) started 3 days prior to cryolesion. Then, soleus muscles were cryolesioned and continued receiving leucine supplementation until 1, 3 and 10 days later. Soleus muscles from leucine-supplemented animals displayed an increase in myofiber size and a reduction in collagen type III expression on post-cryolesion day 10. Leucine was also effective in reducing FOXO3a activation and ubiquitinated protein accumulation in muscles at post-cryolesion days 3 and 10. In addition, leucine supplementation minimized the cryolesion-induced decrease in tetanic strength and increase in fatigue in regenerating muscles at post-cryolesion day 10. These beneficial effects of leucine were not accompanied by activation of any elements of the phosphoinositide 3-kinase/Akt/mechanistic target of rapamycin signalling pathway in the regenerating muscles. Our results show that leucine improves myofiber size gain and strength recovery in regenerating soleus muscles through attenuation of protein ubiquitination. In addition, leucine might have therapeutic effects for muscle recovery following injury and in some muscle diseases.
format	article
author	Marcelo G Pereira Igor L Baptista Eduardo O C Carlassara Anselmo S Moriscot Marcelo S Aoki Elen H Miyabara
author_facet	Marcelo G Pereira Igor L Baptista Eduardo O C Carlassara Anselmo S Moriscot Marcelo S Aoki Elen H Miyabara
author_sort	Marcelo G Pereira
title	Leucine supplementation improves skeletal muscle regeneration after cryolesion in rats.
title_short	Leucine supplementation improves skeletal muscle regeneration after cryolesion in rats.
title_full	Leucine supplementation improves skeletal muscle regeneration after cryolesion in rats.
title_fullStr	Leucine supplementation improves skeletal muscle regeneration after cryolesion in rats.
title_full_unstemmed	Leucine supplementation improves skeletal muscle regeneration after cryolesion in rats.
title_sort	leucine supplementation improves skeletal muscle regeneration after cryolesion in rats.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/9d3e9036147f40b4b71d154cc581e654
work_keys_str_mv	AT marcelogpereira leucinesupplementationimprovesskeletalmuscleregenerationaftercryolesioninrats AT igorlbaptista leucinesupplementationimprovesskeletalmuscleregenerationaftercryolesioninrats AT eduardooccarlassara leucinesupplementationimprovesskeletalmuscleregenerationaftercryolesioninrats AT anselmosmoriscot leucinesupplementationimprovesskeletalmuscleregenerationaftercryolesioninrats AT marcelosaoki leucinesupplementationimprovesskeletalmuscleregenerationaftercryolesioninrats AT elenhmiyabara leucinesupplementationimprovesskeletalmuscleregenerationaftercryolesioninrats
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Leucine supplementation improves skeletal muscle regeneration after cryolesion in rats.

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