A robust and versatile signal-on fluorescence sensing strategy based on SYBR Green I dye and graphene oxide

A robust and versatile signal-on fluorescence sensing strategy based on SYBR Green I dye and graphene oxide

Huazhang Qiu,1,* Namei Wu,1,* Yanjie Zheng,1 Min Chen,2 Shaohuang Weng,1 Yuanzhong Chen,3 Xinhua Lin1 1Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, People’s Republic of China; 2Department of Orthopedic Surgery, Affiliated Union Hospital o...

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Autores principales: Qiu HZ, Wu NM, Zheng YJ, Chen M, Weng SH, Chen YZ, Lin XH
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2014
Materias:
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/9d4031e08d984285896793eef18926db
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spelling oai:doaj.org-article:9d4031e08d984285896793eef18926db2021-12-02T02:43:42ZA robust and versatile signal-on fluorescence sensing strategy based on SYBR Green I dye and graphene oxide1178-2013https://doaj.org/article/9d4031e08d984285896793eef18926db2014-12-01T00:00:00Zhttp://www.dovepress.com/a-robust-and-versatile-signal-on-fluorescence-sensing-strategy-based-o-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Huazhang Qiu,1,* Namei Wu,1,* Yanjie Zheng,1 Min Chen,2 Shaohuang Weng,1 Yuanzhong Chen,3 Xinhua Lin1 1Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, People’s Republic of China; 2Department of Orthopedic Surgery, Affiliated Union Hospital of Fujian Medical University, Fuzhou, People’s Republic of China; 3Fujian Institute of Hematology, The Affiliated Union Hospital of Fujian Medical University, Fuzhou, People’s Republic of China *These authors contributed equally to this work Abstract: A robust and versatile signal-on fluorescence sensing strategy was developed to provide label-free detection of various target analytes. The strategy used SYBR Green I dye and graphene oxide as signal reporter and signal-to-background ratio enhancer, respectively. Multidrug resistance protein 1 (MDR1) gene and mercury ion (Hg2+) were selected as target analytes to investigate the generality of the method. The linear relationship and specificity of the detections showed that the sensitive and selective analyses of target analytes could be achieved by the proposed strategy with low detection limits of 0.5 and 2.2 nM for MDR1 gene and Hg2+, respectively. Moreover, the strategy was used to detect real samples. Analytical results of MDR1 gene in the serum indicated that the developed method is a promising alternative approach for real applications in complex systems. Furthermore, the recovery of the proposed method for Hg2+ detection was acceptable. Thus, the developed label-free signal-on fluorescence sensing strategy exhibited excellent universality, sensitivity, and handling convenience. Keywords: fluorescence, turn-on, SYBR Green I, graphene oxide, multidrug resistance protein 1 gene, Hg2+Qiu HZWu NMZheng YJChen MWeng SHChen YZLin XHDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 147-156 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Qiu HZ
Wu NM
Zheng YJ
Chen M
Weng SH
Chen YZ
Lin XH
A robust and versatile signal-on fluorescence sensing strategy based on SYBR Green I dye and graphene oxide
description Huazhang Qiu,1,* Namei Wu,1,* Yanjie Zheng,1 Min Chen,2 Shaohuang Weng,1 Yuanzhong Chen,3 Xinhua Lin1 1Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, People’s Republic of China; 2Department of Orthopedic Surgery, Affiliated Union Hospital of Fujian Medical University, Fuzhou, People’s Republic of China; 3Fujian Institute of Hematology, The Affiliated Union Hospital of Fujian Medical University, Fuzhou, People’s Republic of China *These authors contributed equally to this work Abstract: A robust and versatile signal-on fluorescence sensing strategy was developed to provide label-free detection of various target analytes. The strategy used SYBR Green I dye and graphene oxide as signal reporter and signal-to-background ratio enhancer, respectively. Multidrug resistance protein 1 (MDR1) gene and mercury ion (Hg2+) were selected as target analytes to investigate the generality of the method. The linear relationship and specificity of the detections showed that the sensitive and selective analyses of target analytes could be achieved by the proposed strategy with low detection limits of 0.5 and 2.2 nM for MDR1 gene and Hg2+, respectively. Moreover, the strategy was used to detect real samples. Analytical results of MDR1 gene in the serum indicated that the developed method is a promising alternative approach for real applications in complex systems. Furthermore, the recovery of the proposed method for Hg2+ detection was acceptable. Thus, the developed label-free signal-on fluorescence sensing strategy exhibited excellent universality, sensitivity, and handling convenience. Keywords: fluorescence, turn-on, SYBR Green I, graphene oxide, multidrug resistance protein 1 gene, Hg2+
format article
author Qiu HZ
Wu NM
Zheng YJ
Chen M
Weng SH
Chen YZ
Lin XH
author_facet Qiu HZ
Wu NM
Zheng YJ
Chen M
Weng SH
Chen YZ
Lin XH
author_sort Qiu HZ
title A robust and versatile signal-on fluorescence sensing strategy based on SYBR Green I dye and graphene oxide
title_short A robust and versatile signal-on fluorescence sensing strategy based on SYBR Green I dye and graphene oxide
title_full A robust and versatile signal-on fluorescence sensing strategy based on SYBR Green I dye and graphene oxide
title_fullStr A robust and versatile signal-on fluorescence sensing strategy based on SYBR Green I dye and graphene oxide
title_full_unstemmed A robust and versatile signal-on fluorescence sensing strategy based on SYBR Green I dye and graphene oxide
title_sort robust and versatile signal-on fluorescence sensing strategy based on sybr green i dye and graphene oxide
publisher Dove Medical Press
publishDate 2014
url https://doaj.org/article/9d4031e08d984285896793eef18926db
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