Anti-Virulence Properties of Coridothymus capitatus Essential Oil against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients

Pseudomonas aeruginosa is an opportunistic pathogen responsible for nosocomial infections, and is often involved in airway infections of cystic fibrosis (CF) patients. P. aeruginosa virulence is related to its ability to form biofilm, trigger different types of...

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Autores principales:	Gianluca Vrenna, Marco Artini, Rino Ragno, Michela Relucenti, Ersilia Vita Fiscarelli, Vanessa Tuccio Guarna Assanti, Rosanna Papa, Laura Selan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Pseudomonas aeruginosa</i> cystic fibrosis essential oil antivirulence SEM motility Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9d40f0c341c747b7ad88daa8b0aa1b3f
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spelling	oai:doaj.org-article:9d40f0c341c747b7ad88daa8b0aa1b3f2021-11-25T18:24:39ZAnti-Virulence Properties of <i>Coridothymus capitatus</i> Essential Oil against <i>Pseudomonas aeruginosa</i> Clinical Isolates from Cystic Fibrosis Patients10.3390/microorganisms91122572076-2607https://doaj.org/article/9d40f0c341c747b7ad88daa8b0aa1b3f2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2257https://doaj.org/toc/2076-2607<i>Pseudomonas aeruginosa</i> is an opportunistic pathogen responsible for nosocomial infections, and is often involved in airway infections of cystic fibrosis (CF) patients. <i>P. aeruginosa</i> virulence is related to its ability to form biofilm, trigger different types of motilities, and produce toxins (for example, bacterial pigments). In this scenario, essential oils (EOs) have gained notoriety for their role in phenotype modulation, including virulence modulation. Among different EOs previously analyzed, herein we investigated the activity of <i>Coridothymus capitatus</i> EO (CCEO) against specific virulence factors produced by <i>P. aeruginosa</i> isolated from CF patients. CCEO showed inhibition of new biofilm formation and reduction in mature biofilm in about half of the tested strains. On selected strains, SEM analysis provided interesting information regarding CCEO action in a pre-adhesion assay. CCEO treatment showed a dramatic modification of the extracellular matrix (ECM) structure. Our results clearly showed a drastic reduction in pyocyanin production (between 84% and 100%) for all tested strains in the presence of CCEO. Finally, CCEO was also able to strongly affect <i>P. aeruginosa</i> swarming and swimming motility for almost all tested strains. In consideration of the novel results obtained on clinical strains isolated from CF patients, CCEO may be a potential candidate to limit <i>P. aeruginosa</i> virulence.Gianluca VrennaMarco ArtiniRino RagnoMichela RelucentiErsilia Vita FiscarelliVanessa Tuccio Guarna AssantiRosanna PapaLaura SelanMDPI AGarticle<i>Pseudomonas aeruginosa</i>cystic fibrosisessential oilantivirulenceSEMmotilityBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2257, p 2257 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Pseudomonas aeruginosa</i> cystic fibrosis essential oil antivirulence SEM motility Biology (General) QH301-705.5
spellingShingle	<i>Pseudomonas aeruginosa</i> cystic fibrosis essential oil antivirulence SEM motility Biology (General) QH301-705.5 Gianluca Vrenna Marco Artini Rino Ragno Michela Relucenti Ersilia Vita Fiscarelli Vanessa Tuccio Guarna Assanti Rosanna Papa Laura Selan Anti-Virulence Properties of <i>Coridothymus capitatus</i> Essential Oil against <i>Pseudomonas aeruginosa</i> Clinical Isolates from Cystic Fibrosis Patients
description	<i>Pseudomonas aeruginosa</i> is an opportunistic pathogen responsible for nosocomial infections, and is often involved in airway infections of cystic fibrosis (CF) patients. <i>P. aeruginosa</i> virulence is related to its ability to form biofilm, trigger different types of motilities, and produce toxins (for example, bacterial pigments). In this scenario, essential oils (EOs) have gained notoriety for their role in phenotype modulation, including virulence modulation. Among different EOs previously analyzed, herein we investigated the activity of <i>Coridothymus capitatus</i> EO (CCEO) against specific virulence factors produced by <i>P. aeruginosa</i> isolated from CF patients. CCEO showed inhibition of new biofilm formation and reduction in mature biofilm in about half of the tested strains. On selected strains, SEM analysis provided interesting information regarding CCEO action in a pre-adhesion assay. CCEO treatment showed a dramatic modification of the extracellular matrix (ECM) structure. Our results clearly showed a drastic reduction in pyocyanin production (between 84% and 100%) for all tested strains in the presence of CCEO. Finally, CCEO was also able to strongly affect <i>P. aeruginosa</i> swarming and swimming motility for almost all tested strains. In consideration of the novel results obtained on clinical strains isolated from CF patients, CCEO may be a potential candidate to limit <i>P. aeruginosa</i> virulence.
format	article
author	Gianluca Vrenna Marco Artini Rino Ragno Michela Relucenti Ersilia Vita Fiscarelli Vanessa Tuccio Guarna Assanti Rosanna Papa Laura Selan
author_facet	Gianluca Vrenna Marco Artini Rino Ragno Michela Relucenti Ersilia Vita Fiscarelli Vanessa Tuccio Guarna Assanti Rosanna Papa Laura Selan
author_sort	Gianluca Vrenna
title	Anti-Virulence Properties of <i>Coridothymus capitatus</i> Essential Oil against <i>Pseudomonas aeruginosa</i> Clinical Isolates from Cystic Fibrosis Patients
title_short	Anti-Virulence Properties of <i>Coridothymus capitatus</i> Essential Oil against <i>Pseudomonas aeruginosa</i> Clinical Isolates from Cystic Fibrosis Patients
title_full	Anti-Virulence Properties of <i>Coridothymus capitatus</i> Essential Oil against <i>Pseudomonas aeruginosa</i> Clinical Isolates from Cystic Fibrosis Patients
title_fullStr	Anti-Virulence Properties of <i>Coridothymus capitatus</i> Essential Oil against <i>Pseudomonas aeruginosa</i> Clinical Isolates from Cystic Fibrosis Patients
title_full_unstemmed	Anti-Virulence Properties of <i>Coridothymus capitatus</i> Essential Oil against <i>Pseudomonas aeruginosa</i> Clinical Isolates from Cystic Fibrosis Patients
title_sort	anti-virulence properties of <i>coridothymus capitatus</i> essential oil against <i>pseudomonas aeruginosa</i> clinical isolates from cystic fibrosis patients
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9d40f0c341c747b7ad88daa8b0aa1b3f
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Anti-Virulence Properties of <i>Coridothymus capitatus</i> Essential Oil against <i>Pseudomonas aeruginosa</i> Clinical Isolates from Cystic Fibrosis Patients

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