MK8722, an AMPK activator, inhibiting carcinoma proliferation, invasion and migration in human pancreatic cancer cells

Background: MK8722 is a potent and systemic pan-AMPK activator. It is an effective, direct, allosteric activator of AMPK complex in many mammals. This study tried to explore the underlying anti-cancer molecular mechanism of MK8722 in human pancreatic cancer cells (PCCs). Methods: The anti-proliferat...

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Autores principales:	Cheng Wang, Baojun Huang, Linxiao Sun, Xi Wang, Baofeng Zhou, Hongli Tang, Wujun Geng
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	MK8722 AMPK Pancreatic cancer Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/9d4138ad7a914c57a0a12d3a87ec392e
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spelling	oai:doaj.org-article:9d4138ad7a914c57a0a12d3a87ec392e2021-11-14T04:29:43ZMK8722, an AMPK activator, inhibiting carcinoma proliferation, invasion and migration in human pancreatic cancer cells0753-332210.1016/j.biopha.2021.112325https://doaj.org/article/9d4138ad7a914c57a0a12d3a87ec392e2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221011094https://doaj.org/toc/0753-3322Background: MK8722 is a potent and systemic pan-AMPK activator. It is an effective, direct, allosteric activator of AMPK complex in many mammals. This study tried to explore the underlying anti-cancer molecular mechanism of MK8722 in human pancreatic cancer cells (PCCs). Methods: The anti-proliferation, invasion and migration functions of MK8722 in human pancreatic cancer analyzed by real time cellular analysis, colony formation assay, cell migration assay, transwell assay and flow cytometery analysis. Moreover, the potential targeted signaling pathway was tested via RNA-seq and pathway enrichment analysis. Results: In the present study, we investigated the anti-PCCs effects of MK8722 on two different human pancreatic cancer cell lines (PANC-1 and Patu8988). The results showed that MK8722 significantly inhibited human tumor cells proliferation and migration/invasion in a dose-dependent manner. Additionally, the influence of MK8722 was examined by analyzing the expression of potential key genes and pathways, which may provide novel insights to the mechanism of MK8722. Conclusion: The inhibition of pancreatic cancer by MK8722 through a number of pathways that inhibit carcinoma proliferation, invasion and migration. The potential effect of MK8722 might be determined by regulating the expression of AL162151, IER2, REPIN1, KRT80 to inhibit cycle arrest and migration.Cheng WangBaojun HuangLinxiao SunXi WangBaofeng ZhouHongli TangWujun GengElsevierarticleMK8722AMPKPancreatic cancerTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 144, Iss , Pp 112325- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	MK8722 AMPK Pancreatic cancer Therapeutics. Pharmacology RM1-950
spellingShingle	MK8722 AMPK Pancreatic cancer Therapeutics. Pharmacology RM1-950 Cheng Wang Baojun Huang Linxiao Sun Xi Wang Baofeng Zhou Hongli Tang Wujun Geng MK8722, an AMPK activator, inhibiting carcinoma proliferation, invasion and migration in human pancreatic cancer cells
description	Background: MK8722 is a potent and systemic pan-AMPK activator. It is an effective, direct, allosteric activator of AMPK complex in many mammals. This study tried to explore the underlying anti-cancer molecular mechanism of MK8722 in human pancreatic cancer cells (PCCs). Methods: The anti-proliferation, invasion and migration functions of MK8722 in human pancreatic cancer analyzed by real time cellular analysis, colony formation assay, cell migration assay, transwell assay and flow cytometery analysis. Moreover, the potential targeted signaling pathway was tested via RNA-seq and pathway enrichment analysis. Results: In the present study, we investigated the anti-PCCs effects of MK8722 on two different human pancreatic cancer cell lines (PANC-1 and Patu8988). The results showed that MK8722 significantly inhibited human tumor cells proliferation and migration/invasion in a dose-dependent manner. Additionally, the influence of MK8722 was examined by analyzing the expression of potential key genes and pathways, which may provide novel insights to the mechanism of MK8722. Conclusion: The inhibition of pancreatic cancer by MK8722 through a number of pathways that inhibit carcinoma proliferation, invasion and migration. The potential effect of MK8722 might be determined by regulating the expression of AL162151, IER2, REPIN1, KRT80 to inhibit cycle arrest and migration.
format	article
author	Cheng Wang Baojun Huang Linxiao Sun Xi Wang Baofeng Zhou Hongli Tang Wujun Geng
author_facet	Cheng Wang Baojun Huang Linxiao Sun Xi Wang Baofeng Zhou Hongli Tang Wujun Geng
author_sort	Cheng Wang
title	MK8722, an AMPK activator, inhibiting carcinoma proliferation, invasion and migration in human pancreatic cancer cells
title_short	MK8722, an AMPK activator, inhibiting carcinoma proliferation, invasion and migration in human pancreatic cancer cells
title_full	MK8722, an AMPK activator, inhibiting carcinoma proliferation, invasion and migration in human pancreatic cancer cells
title_fullStr	MK8722, an AMPK activator, inhibiting carcinoma proliferation, invasion and migration in human pancreatic cancer cells
title_full_unstemmed	MK8722, an AMPK activator, inhibiting carcinoma proliferation, invasion and migration in human pancreatic cancer cells
title_sort	mk8722, an ampk activator, inhibiting carcinoma proliferation, invasion and migration in human pancreatic cancer cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/9d4138ad7a914c57a0a12d3a87ec392e
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_version_	1718430031496085504

MK8722, an AMPK activator, inhibiting carcinoma proliferation, invasion and migration in human pancreatic cancer cells

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