A Universal, Genomewide GuideFinder for CRISPR/Cas9 Targeting in Microbial Genomes

ABSTRACT The CRISPR/Cas system has significant potential to facilitate gene editing in a variety of bacterial species. CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) represent modifications of the CRISPR/Cas9 system utilizing a catalytically inactive Cas9 protein for transcription rep...

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Bibliographic Details
Main Authors: Michelle Spoto, Changhui Guan, Elizabeth Fleming, Julia Oh
Format: article
Language:EN
Published: American Society for Microbiology 2020
Subjects:
Online Access:https://doaj.org/article/9d416f30bed54a0f8705132a8fd21858
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